Aims: This study was aimed to characterize laccase from a selected fungal strain and examine the enzyme’s ability to remove lignin from paper pulp. Methodology and results: Twelve fungal strains were screened for laccase production, resulting in the selection of Trichoderma sp. UBDFT12. The highest laccase activity (103 U/L) was observed from the culture filtrate on the fourth day of incubation. The optimum temperature and pH for the enzyme were 40 °C and pH 4, respectively. However, the enzyme stability was found to be reduced with time after 1 h incubation. At 1 mM, it was found that AgNO3, CaCO3, CuSO4, KCl, MgSO4, MnSO4 and ZnSO4 increased the laccase activity to 107, 107, 111, 112, 106, 105 and 107%, respectively, whereas FeSO4 and NH4Cl reduced the activity to 84 and 99%, respectively. The addition of 1% H2O2, 1% NaCl, 1% sodium dodecyl sulphate (SDS), 1 mM ethylenediaminetetraacetic acid (EDTA), 10 mM EDTA, 1 mM phenanthroline and 10 mM phenanthroline reduced the activity to 95, 73, 0, 79, 79, 73 and 37%, respectively. The culture filtrate was partially purified via ammonium sulphate precipitation and the recovered enzyme had a specific activity of 0.176 U/mg. Using sodium dodecyl-sulfate polyacrylamide gel electrophoresis (SDS-PAGE), the molecular weight of the enzyme was approximately 65 kDa and its activity was confirmed by zymography. The culture filtrate was also found to be able to remove lignin from different types of paper pulp. Conclusion, significance and impact of study Laccase produced by Trichoderma sp. UBDFT12 was found to have the ability to remove lignin from paper pulp.

In Malaysia, dengue fever is a common vector-borne disease. Most cases of dengue fever are reported in residential areas. Children are at high risk to be infected because they spend a lot of time around their housing area for outdoor activities. Such areas may have a high number of Aedes mosquitoes. As schools are equipped with infrastructure and medium for effective educational purposes, school represent the most suitable facilities for learning and teaching process. A cross-sectional study was conducted to determine the level of knowledge among students in Hulu Langat on Aedes mosquitoes, dengue fever, and Zika virus. The study also reported on the demographic factors which affected the knowledge level. Based on sample size calculation using G*Power v3.1 software, a total of 171 respondents were needed from the two primary schools involved in the study. Data collection was conducted using a pre-tested questionnaire that consisted of two main sections on demography and knowledge about Aedes mosquitoes, dengue fever and Zika virus. From the total score of 55, the mean knowledge score is 34.2 ± 8.38. Based on the median value of 36.0, about 50.9% of respondents had satisfactory knowledge. From the study, it was found that students from School A, students who had been watching video related to Aedes mosquito, and those with history of dengue infection among family members had a significantly better knowledge score (p < 0.01). In conclusion, knowledge about Aedes mosquitoes and related illnesses need to be enhanced through a comprehensive module and effective educational materials that can serve as a reliable source of information for primary school students.