Objective: To analyze the feasibility of axillary lymph node staging through sentinel lymph node biopsy (SLNB) after neoad-juvant chemotherapy (NAC) in patients with node-positive breast cancer and to explore the follow-up treatment of these patients. Methods: Clinical data of 82 patients with node-positive breast cancer before NAC in Tianjin Medical University Cancer Institute and Hospital from January 2016 to January 2018 were analyzed retrospectively. All these patients accepted SLNB after NAC. The detection rate, accuracy, false negative rate (FNR), and influencing factors were analyzed. Results: A nodal pathological complete response (PCR) was achieved in 43 of 82 patients. The PCR rate was 52.4%. The detection rate, accuracy, and FNR were 97.56% (80/82), 88.75% (71/80), and 23.08% (9/39), respectively. The accuracy of 1, 2, and≥3 SLNs detected were 90.9% (20/22), 66.7% (10/15), and 95.3% (41/43), respectively. The FNRs were 20.0% (2/10), 71.4% (5/7), and 9.1% (2/22), respectively (both P<0.05). Conclusions: Due to its overall high FNR, without clinically acceptable limits, post-NAC SLNB cannot completely replace axillary lymph node dissection (ALND) in node-positive patients. However, with no less than 3 SLNs detected, SLNB can accurately evaluate the status of axillary lymph nodes.

Objective To investigate the effects of a new c-Met inhibitor SU11274 on apoptosis and motility of c-Met-positive basal-like breast cancer cells MDA-MB-231. Methods The concentrations of SUl1274 were set to 0,0.1,1,10 and 20 μmol/L.Morphological change of apoptotic cells was analyzed by Hoechst33342,MitroTrackerRed and Yo-pro-1 staining.The apoptotic rate of MDA-MB-231 cells were determined by Annexin V/PI double-staining. The expression of apoptosis related proteins (Bcl-XL,Caspase-3 and PARP) and phosphorylation levels of c-Met and Akt were analyzed by Western blot.The capability of motility were measured by wound-healing assay and chemotaxis assay. Results After treatment by SU11274( 10 μmol/L) for 48 h,shrinking apoptotic cells of MDA-MB-231 was observed by flurescent microscope and nuclear fragmentation was seen.Annexin V/PI double-staining showed SU11274induced apoptosis of MDA-MB-231 cells (P ＜ 0.05 ),and the apoptotic rates were (7.3 ± 0.9) ％,( 14.1 ±0.6) ％,(35.5 ± 4.4) ％ and (48.2 ± 5.3 ) ％,respectively.SU11274 downregulated the expression of Bcl-XL and promoted the dissection of Caspase-3 and PARP in a dose dependent relationship.SU11274 prolongs the wound-healing time,decreases the migration cell count (P ＜ 0.05 ) and effectively inhibits the phosphorylation of c-Met and its downstream key proteins Akt in a dose-dependent manner.Conclusions C-Met inhibitor SU11274 induces apoptosis and inhibits the motility of c-Met-positive basallike breast cancer cell line MDA-MB-231,probably through inhibiting phosphorylation of c-Met/PI3K/Akt.

Objective To investigate the correlation between myocardial microcirculation and inflammation in acute myocardial infarction(AMI)by myocardial contrast echocardiography(MCE).Methods Eighty-one AMI patients(AMI group)and 30 healthy adults(normal control group)were enrolled.White blood cell(WBC),hypersensitive C reactive protein(hsCRP)and neutrophilic gtanulocyte percentage(G%)were measured in patients and healthy adults.According to different count of WBC,AMI group was devided into A group(n=40,WBC≥10×109/L)and B group(n=41,WBC＜10×109/L).Myocardial contrast intensity(A),blood flow rate(β)and myocardial blood volume(MBF=A×β)of each segment were measured by MCE during 5-7 days of hospitalization.Results Compared with control group.WBC,hsCRP in A group and hsCRP in B group were significantly elevated(P＜0.05).A,β and MBF in A group were significantly lower than those in B group(P＜0.05).WBC and hsCRP in A group had significant negative correlations with β respectively(BWBC=-0.67,P＜0.05;BhsCRP=-0.5 1,P＜0.05).Conclusions The level of plasma inflammatory factors were remarkably increased after AMI,which had a positive correlation with low microcirculation perfusion.

Based on abundant experiences on circuit servicing of medical equipment in frontier defending military units,this paper discusses the significance of military medical equipment third-class servicing station again and provides solutions to some problems in work.

Objective To discuss the principle and clinical application of microcomputer optometer.Methods The optometer is composed of singlechip,handle and LCD.The eyesight is examined by selecting exposure of random sample eye-chart mark 'E' within direction keys on the handle.Results The optometer has the merits of simple manipulation,exact result,small cubage,AC/DC power supply and so on.Besides,it prevents the testee from remembering eye-chart.Conclusion The optometer can be extensively used in hospital,school,every work unit or even family.

Objective To study the effect of medical equipment on the first-aid and treatment for field wound in modern war.Methods Problems were collected and analyzed in inspecting medical equipment in frontier defense army.Results The development and quality of medical equipment affect the modern war and the health of commanders in different degrees.Conclusion Aiming at problems of military medical equipment,problems and countermeasures are proposed in the first-aid and treatment of field wound for the future.

Objective To discuss how to improve the management and using efficiency of first-aid equipment.Methods The scientific management of hospital first-aid equipment was discussed in such aspects as administrator,duty,equipment,metrology and personnel.Results The management level and using efficiency of hospital first-aid equipment were greatly improved.Conclusion The method improves the first-aid and synthesis capability.

To make biomedical engineer comprehend deeply the cleaning and disinfection method of hemodialysis equipment,this paper discusses two factors determining cleaning and disinfection method,analyzes the impurities infecting dialysis' quality and treatment effect in hemodialysis system,and systematically introduces the daily cleaning and disinfection of hemodialysis equipment as well as its notice by citing Gambro AK95 series hemodialysis equipment.

Objective To introduce the function characteristics and clinical application of PET/CT.Methods The technical performance of PET/CT was analyzed and its development trend was expected.Results PET/CT reflected physiology,pathology,biochemistry and metabolism of physical tissue at molecule level,and multi-slice CT images displayed subtle anatomic structure.Conclusion PET/CT achieves the combination of PET and CT images,which have significant diagnosis capability and clinical application value.

Objective To introduce the display principle and clinical application of PET-CT. Methods The display principle of PET-CT was analyzed. Results The status of tissue or organ were showed by using PET in the form of dissection images and the relative physiological parameters. Advanced CT technology exactly locates and indicates anatomic and functional information of target tissue or organ. Conclusion In combination of PET and CT, PET-CT show high value in clinical application.