Objective:To explore therapeutic efficacy and mechanism of puerarin(PUE)in treating of ulcerative colitis(UC).Methods:Network pharmacology and molecular docking technique were used to screen and analyze targets of PUE in regulating UC.C57BL/6 mice were given free access to 2.5%DSS aqueous solution for 7 days,and influence of PUE on changes in body weight and disease activity index(DAI)score were subsequently observed.Histopathological alterations of colon tissue were observed by HE staining,changes of goblet cell population in colon tissue were evaluated through Alcian blue staining;expressions of inflammatory factors in colon tissue were detected by qRT-PCR and ELISA.Effect of PUE on MODE-K cell viability and apoptosis were assessed by CCK-8 and flow cytometry.Results:A total of 38 common targets of PUE in modulating UC,such as AKT1,TNF,STAT3,CASP3,HIF1A and etc,mainly involving TNF,IL-17 and PI3K-Akt signaling pathway.In vivo experiments confirmed that PUE ameliorated degree of colon shortening,body weight and DAI scores and reduced inflammatory cell infiltration in mice.Besides,expressions of inflammatory factors in colon,such as TNF-α and IL-1β,were inhibited by PUE.Furthermore,in vitro experiments validated that PUE relieved DSS-induced apoptosis of epithelial cells.Conclusion:PUE alleviates occurrence and development of DSS-induced UC in mice.

The rapid development of scientific and technological innovation has gradually brought science and technology ethics into the public view.The innovation subjects are the main force of scientific and technological innovation,and building a sound science and technology ethics governance system must enhance their science and technology ethics governance abilities.The current situation of science and technology ethics governance of 182 innovation subjects in Anhui province was investigated from six aspects.The results showed that Anhui Province had achieved preliminary results in promoting the construction of a science and technology ethics governance system,but there were still many problems and shortcomings.Based on the results of the investigation and analysis,this paper proposed six countermeasures and suggestions,including establishing a sound technology ethics(review)committee for innovation subjects,valuing the registration of science and technology ethics information for innovation subjects,accelerating the science and technology ethics governance in the field of artificial intelligence,regularly conducting the science and technology ethics learning and training,cultivating compound the science and technology ethics management talents,and strengthening research on the science and technology ethics governance issues,with a view to enhancing the ability of science and technology ethics governance for innovation subjects,improving the construction of science and technology ethics governance system,and assisting the healthy development of science and technology innovation in the whole province.