Objective To investigate the effects of pulmonary artery denervation (PADN)on pulmonary artery function outside of the nervous system.Methods Thirty-six beagles of 8-10 months old weighting 8-15 kg were randomized into six groups:a blank control group,an immediate post-surgery group,a 1 month post-surgery group,a 2 months post-surgery group,a 3 month post-surgery group,and a pulmonary artery hypertension(PAH)group,with six dogs in each group.Dogs in the blank control group received no treatment,and dogs in the surgery groups were treated with PADN and were then killed immediately after surgery,at 1,2 or 3 months after surgery.PAH dogs were induced by dehydromonocrotaline,and they were killed at 8 weeks after treatment.Protein levels of angiotensin Ⅱ (Ang Ⅱ),angiotensin converting enzyme (ACE),angiotensin converting enzyme 2 (ACE2),mineralocorticoid receptors (MR)and antibodies against aldosterone in dog pulmonary and right ventricular tissues were detected by Western blot.The mRNA expression of the Ang Ⅱ type 1 receptor(AT1R)was determined by real-time reverse transcription quantitative polymerase chain reaction(RT-qPCR).The protein expression ratio of ACE2 to ACE in lung and right ventricular tissues were calculated.Changes of the renin-angiotensin-aldosterone system (RAAS)in lung and right ventricular tissues were compared between the groups.Results In pulmonary tissues,protein levels of Ang Ⅱ,ACE2,MR,and AT-1R increased immediately after PADN (P =0.03,0.03,0.01 and ＜ 0.01),and the protein expression of ACE decreased(P ＜0.001),indicating that RAAS was activated immediately after PADN.Three months after PADN,Ang Ⅱ protein levels declined significantly in lung tissues(P =0.030),and ACE2,ACE,MR,and AT-1 receptor protein expression had no obvious difference(P =0.670,0.570,0.180 and 0.349),compared with the blank control group.In right ventricular tissues,the protein expression of ACE2 was higher in the surgery groups than in the blank control group(P =0.0161),and there was no significant difference in the protein expression of Ang Ⅱ,ACE,MR,and AT-1 receptors(P=0.641,0.134,0.227 and 0.330).Conclusions RAAS activates immediately in dogs with normal pulmonary pressure after PADN,the damaging factors in pulmonary tissues are reduced and the vascular protective factors in right ventricular tissues are elevated at 3 months after PADN.

Objective To discuss and analyze the clinical relationships between the gene polymorphism of vitamin D receptor and adiponectin with the susceptibility of non-alcohol fatty liver disease(NAFLD).Methods One hundred and two cases of NAFLD were selected as the observation group,and other 100 healthy volunteers were selected as the control group.The gene polymorphism of vitamin D receptor and adiponectin in the two groups was detected,then the genotype distribution and allele frequencies of vitamin D receptor and adiponectin were compared between the two groups,then their relationship with the susceptibility of NAFLD was analyzed.Results The genotype distribution situation of vitamin D receptor BsmI site,adiponectin 45 and 276 sites had statistically significant difference between the observation group and control group(P＜0.05).The B allele frequency of BsmI site of vitamin D receptor in the observation group was far lower than that in the control group,and the allele frequencies of 45-T and 276-G of vitamin D receptor in the former were far higher than those in the latter,and the differences between the two groups were statistically significant(P＜0.05).The multivariate unconditional Logistic regression analysis showed that vitamin D receptor:bb genotype,adiponectin 45 locus:TT genotype and ALT,TG,complicating hypertension history and HOMA-IR all were the independent risk factors in NAFLD patients.Conclusion The genotype distribution of different sites of vitamin D receptor and adiponectin has obvious abnormality in the patients with NAFLD,and both are closely related with the NAFLD susceptibility.