Objective:To investigate the efficacy and safety of non-penetrating trabecular surgery combined with nearly 360-degree suture trabeculotomy for the treatment of primary congenital glaucoma (PCG).Methods:An observational case series study was conducted.A total of 29 cases (50 eyes) with PCG, including 21 males (35 eyes) and 8 females (15 eyes), were enrolled in Jiangsu Province Hospital and Nanjing Children's Hospital from January to November, 2019.The age of subjects ranged from 1 month to 4 years, and the median age was 6 months.Non-penetrating trabecular surgery was first performed in order to open the Schlemm canal.The cannulation and nearly 360-degree suture trabeculotomy were then performed with the twisted 6-0 polypropylene suture.Intraocular pressure (IOP), corneal diameter, cup-to-disc ratio (c/d) and complications were recorded preoperatively and 1 week, 1 month, 3 months, 6 months, 9 months, 12 months, and 24 months postoperatively, and the proportion of sutures successfully passed through the Schlemm canal and the success rate of operation were recorded.This study followed the Declaration of Helsinki.The study protocol was approved by an Ethics Committee of The First Affiliated Hospital of Nanjing Medical University (No.2019-SR-198). Written informed consent was obtained from the guardian of each subject prior to entering the study cohort.Results:Circumferential cannulation by suture was successfully performed in 90% of the subjects.The Harms trabeculotomy probe was applied in failed cases.Mean IOP was significantly lowered from preoperative (35.0±9.5) mmHg (1 mmHg=0.133 kPa) to (9.9±4.4), (10.0±4.2), (9.7±4.4), (9.0±2.9), (9.4±4.2), (9.3±3.3) and (9.5±3.8) mmHg at postoperative 1 week, 1 month, 3 months, 6 months, 9 months, 12 months and 24 months, respectively ( F=141.56, P<0.01). Mean corneal diameter was significantly reduced from preoperative (13.7±1.4) mm to (13.3±1.4), (12.9±1.4), (12.8±1.3), (12.7±1.2), (12.6±1.1), (12.6±1.1) and (12.8±0.4) mm at postoperative 1 week, 1 month, 3 months, 6 months, 9 months, 12 months and 24 months, respectively ( F=4.55, P<0.01). Mean c/d was significantly reduced from preoperative 0.81±0.15 to 0.55±0.22, 0.48±0.23, 0.45±0.22, 0.43±0.21, 0.41±0.20, 0.40±0.21 and 0.31±0.19 at postoperative 1 week, 1 month, 3 months, 6 months, 9 months, 12 months and 24 months, respectively ( F=21.07, P<0.01). Forty-two eyes (93.3%) achieved complete success and 45 eyes (100%) achieved qualified success at postoperative 12 months.No severe complications were observed during or after surgery. Conclusions:Non-penetrating trabecular surgery combined with nearly 360-degree suture trabeculotomy can effectively treat patients with PCG without any severe complications.

Objective To investigate the effects of matrine on proliferation and apoptosis of human Tenon capsule fibroblasts (HTFs) in.vitro.Methods After treated with 0,0.3,0.6 and 0.9 g/L matrine in vitro,cell counting kit-8 (CCK-8) method was used to assay the proliferation of HTFs at 24,48 and 72 hours,Western blot and PCR were performed to evaluate the expression of apoptosis-associated factor caspase-3 on both protein and RNA levels.Results The activity of human Tenon capsule fibroblast at 48 hours and 72 hours after treated with 0.3,0.6,0.9 g/L matrine was significantly inhibited when compared with the 0 g/L matrine group,and the inhibitory effect was dose-dependent and time-dependent (F ion =1 019.51,P =0.00;Ftime =5 848.66,P =0.00;Fi ion =147.45,P=0.00).After treated with 0,0.3,0.6 and 0.9 g/L matrine,the early apoptosis rate of HTFs was (2.68±0.30)％,(5.08±0.47)％,(6.97±0.69)％ and (10.30±1.20)％,the grey value ofcaspase-3 protein was 1.00±0.13,1.90±0.19,2.50±0.30 and 2.67±0.30,the relative expression of caspase-3 mRNA was 0.98 ±0.12,2.01 ±0.34,6.15 ± 0.60 and 11.40 ± 1.12,respectively,with significant differences among them (F =55.74,66.01,154.50;all at P＜0.01),the early apoptosis rate of HTFs,the grey value of caspase-3 protein and the relative expression of caspase-3 mRNA were all increased significantly as the concentration of matrine increased,with significant differences between any two groups (all at P＜0.05).Conclusions Matrine can inhibit the proliferation of HTFs and induce the apoptosis of HTFs in a time-and dose-depended manner.

OBJECTIVETo compare the effects between acupuncture with regulating mind and spleen and wes-tern medication for diarrhea irritable bowel syndrome (IBS-D).

METHODSEighty-one patients were randomly at the ratio of 2 to 1 assigned into an acupuncture group (54 cases) and a western medication group (27 cases). Acupuncture with regulating mind and spleen was applied in the acupuncture group for 6 weeks at Baihui (GV 20), Yintang (GV 29), Tianshu (ST 25), Zusanli (ST 36), Shangjuxu (ST 37), Sanyinjiao (SP 6), and Taichong (LR 3), once every other day, 3 times a week. Pinaverium bromide tablet was used orally in the western medication group for 6 weeks, 50 mg a time, 3 times a day. IBS symptom severity score (IBS-SSS) were observed before and after 1-week, 6-week treatment. Pittsburgh sleep quality index (PSQI) was applied before and after 6-week treatment. Also, clinical efficacy was evaluated in the two groups.

RESULTSThree patients dropped out in the acupuncture group, and 1 in the western medication group. Except abdominal distension score after 1-week treatment in the western medication group, single scores and total scores of IBS-SSS apparently reduced in the two groups after 1-week and 6-week treatment (<0.01,<0.05). After 1-week treatment, the abdominal pain score of the acupuncture group was obviously lower than that of the western medication group (<0.05). After 6-week treatment, with abdominal pain relief advantage, other results including the number of pain days, defecation satisfaction,life disturbance degree and total score of the acupuncture group were obviously lower than those of the wes-tern medication group (<0.01,<0.05). After 6-week treatment, the PSQI score and its change before and after treatment in the acupuncture group were superior to those in the western medication group (both<0.05). The relief rate and relief plus obvious effective rate in the acupuncture group were higher than those in the western medication group[51.0% (26/51) vs 19.2% (5/26),<0.01; 64.7% (33/51) vs 34.6% (9/26),<0.05].

CONCLUSIONSAcupuncture with regulating mind and spleen for diarrhea irritable bowel syndrome can more effectively relieve abdominal pain than pinaverium bromide tablet at the early stage. Its total effect and single effects are better at the later stage on abdominal pain, seizure frequency, defecation satisfaction, life disturbance, and sleep quality.

Myelodysplastic syndromes are a group of heterogeneous myeloid tumors and the prognosis is affected by a variety of factors.The pathophysiology of myelodysplastic syndromes (MDS) is unclear.Recent studies have shown that MDS patients have extensive genetic abnormalities.With the development of new genome sequencing technology,abnormal genes can be found in about 80％ of MDS patients.These molecular biological abnormalities have the prospect of guiding targeted therapy and disease surveillance,and can give a hope for the individualized management of heterogeneous hematological malignancies.Here,the prognostic factors of MDS,especially for MDS molecular biology in recent years have been reviewed.

Objective The aim of this study was to evaluate the glioma using the variety of functional magnetic resonance imaging( fMRI) ,and to perform a more accurate preoperative diagnosis of gliomas. Methods Thirty - five patients with gliomas confirmed by pathology were examined by magnetic resonance imaging (MRI)and functional MRI. Rapid diffusion coefficient(D?),Slow diffusion coefficient(D),perfusion fraction ( f) and distribution diffusion coefficient ( DDC ) in the intravoxel incoherent motion diffusion weighted imaging (IVIM-DWI) were analyzed statistically. Results The mean values of D?,D,f and DDC in the IVIM se-quence of the patients with high-grade of gliomas were statistically significant when compared to the IVIM values of the contralateral normal brain tissues(P<0. 05). Conclusion A variety of magnetic resonance functional im-aging sequences are used to analyze gliomas,which can avoid tumor heterogeneity and improve the recognition a-bility and accuracy of magnetic resonance imaging in high grade gliomas.

Objective To analyze the current status and the issues of alexithymia's nursing in China to provide references for further research in this area.Methods CNKI, Wanfang, VIP, CBM, PubMed and CINAHL databases were screened for published papers on alexithymia's nursing in China from their inauguration to 30th September 2016. Bibliometric data were extracted and analyzed using metrological analysis.Results A total of 84 papers were included. The first paper was published in 2001. The amount of the literature increased annually after 2009. Papers were mainly from the central and eastern regions and lack of foundation. The major papers were descriptive studies and accounted for 59.52%. The main topic was investigation of correlations between alexithymia and psychosomatic symptoms and symptoms of disease, which accounted for 48.84%. Subjects in the study were mainly patients with chronic diseases with a rate of 23.81%. In 23 included clinical trials, alexithymia's nursing was mainly performed in forms of group psychological counseling with a rate of 26.09%. The less common form was realization social support which accounted for 17.39%. The evaluation parameters contained 78.26% of functional indexes of patients and 21.74% of clinical outcome indicators.Conclusions The published papers are in small amount and with low quality. It is necessary to expand the studies on the specialized diseases and establish multi-dimension nursing model based on the combination of nursing theory and clinical practice.

Objective To investigate the status quo of humanistic caring ability of undergraduate nursing students, and to analyze the influencing factors.Methods By convenient sampling method, 171 students majoring in Nursing, enrolled to School of Nursing, Shanxi University of Traditional Chinese Medicine in 2015, were selected and surveyed by Caring Ability Inventory (CAI) about their humanistic caring ability and influencing factors.Results In the scale CAI, total scores, scores of dimension courage, and scores of dimension patience of the nursing students were (185.29±23.32), (58.30±9.91), and (56.94±9.35), lower than the norm (208.58±23.32), (65.25±11.57) and (63.11±4.19) (t/Z=-12.907, -9.068, 2.229;P<0.001). Scores of students, who were the only child in their family, on the dimension courage and patience were (55.17±10.51) and [57(46,61)], lower than those who came from non-only-child family (59.16±9.59) and [59(55,63)], (t/Z=-2.166, -2.651;P<0.05).Conclusions Overall humanistic caring ability of nursing students in our country is relatively low. Educators should pay attention to related factors, formulate targeted training strategies, so as to strengthen education on humanistic caring of nursing students.

To quantify the transcriptional activity of NF-κB and to screen drugs related to the regulation of NF-κB activation, we constructed a recombinant plasmid through deleting the original CMV promoter of retrovirus vector pQCXIP and inserting the NF-κB enhancer and NanoLuc luciferase sequence into the vector. Then, using the recombinant plasmid we constructed a cell line in which the expression of NanoLuc luciferase (NLuc) was regulated by NF-κB. The inserted sequences were verified by restriction endonuclease digestion and sequencing. Tumor necrosis factor-α (TNF-α), an NF-κB activator, acted on the constructed NLuc cell line and leaded to the specific luciferase reaction. The luciferase reaction showed a fine time and dose dependence to the TNF-α stimulation, indicating the successful construction of the NF-κB regulated NLuc-expressing cell line. Besides, the NF-κB inhibitor, triptolide, reduced the expression of NLuc in a dose-dependent way. The constructed reporter system in this study could be applied in the quantification of the NF-κB transcriptional activity and in the NF-κB regulation-related drug screening.

Objective To explore the effect of nursing of Traditional Chinese Medicine on cognitive function among aged people with mild cognitive impairment in elderly care institution. Methods A total of 66 cases with mild cognitive impairment elderly that previously were screened out from two pension institutions in Taiyuan city as the research objects. According to the endowment institutions where the objects of study dwelled was divided into control group (34 cases) and intervention group (32 cases). The intervention group accepted nursing of Traditional Chinese Medicine plan for four months. The control group received the disease related knowledge health education. The cognitive function of the two groups were assessed using the Montreal Cognitive Scale (MoCA) before and after the intervention. Results Before the intervention, two groups had no statistical significance in each dimension score and total score of MoCA. After the intervention, four dimensions scores including ability of visual spatial and execute , attention , delayed recall , directional force and the total score of the intervention group respectively were (2.67±1.09) points, (4.07±1.08) points, (1.97±0.81) points, (4.83±0.99) points, (18.80±1.74) points while the control group were (2.15 ± 0.71) points, (3.30 ± 0.63) points, (1.39 ± 0.97) points, (4.24 ± 1.41) points, (15.06 ± 2.98) points, and the difference was statistically significant (t=1.91-6.06, P < 0.05 or 0.01) ;Before intervention the language score, delayed recall score and the total score of the intervention group were (1.43±0.77) points, (1.57±1.10) points, (17.27±1.99) points, respectively while after the intervention were (1.80 ± 0.66) points, (1.97 ± 0.81) points, (18.80 ± 1.74) points, there was statistically significant difference (t =-2.16,-2.11,- 4.34, P < 0.05 or 0.01). Conclusions Nursing of Traditional Chinese Medicine can delay the process of cognitive decline of mild cognitive impairment in the elderly, and have a certain effect on prevention and treatment of patients with mild cognitive impairment.

Objective To investigate the self-transcendence of the elderly patients with type 2 diabetes,and analyze the influencing factors. Methods A convenient sample design was used and a random questionnaire survey was conducted among 211 community elderly patients with type 2 diabetes for the current situation and influencing factors of self-transcendence in Taiyuan, from August to October 2014. Results The total score of self-transcendence was (41. 03 ± 4. 64). The surveys showed that the influencing factors of elderly patients′self-transcendence level were marital status, education level, occupation before retirement, monthly income, living conditions, personality characteristics, participation in collective activities, a significant event in half year or not, diabetes process, combined chronic diseases conditions, situation of diabetes complications and health. Further Multiple linear stepwise regression analysis showed that education level, living conditions, participation in collective activities, monthly income, diabetes duration were the main influencing factors ( R=0. 529,R2 =0. 280,F=13. 191;P<0. 01). Conclusions Through the concern of the self-transcendence of the elderly patients with type 2 diabetes, it provides the theoretical basis for future intervention measures, and finally makes the elderly patients with types 2 diabetes who dare to face the negative events in their lives, adjust the state of life, set up the positive outlook on life, continuously surpass ourselves to mature, so as to finally achieves groups of healthy aging and active aging.