Objective To explore the characteristics of sleep structure in chronic insomnia disorder(CID)with disharmony between heart and kidney pattern and liver qi stagnation pattern.Methods A total of 74 patients with CID who were treated in Tongde Hospital of Zhejiang Province from June 2021 to December 2022 were selected and divided into disharmony between heart and kidney group and liver qi stagnation group according to TCM syndrome differentiation types,with 37 cases in each group.Relevant parameters of both groups were collected and analyzed using polysomnography(PSG).Results The age of patients in disharmony between heart and kidney group was significantly higher than that in liver qi stagnation group(P<0.05).There were statistically significant differences in total sleep time,sleep efficiency,rapid eye movement sleep period,N1 stage and N3 stage between two groups(P<0.05).The results indicated that patients in disharmony between heart and kidney group had light sleep,low sleep efficiency and short total sleep time.Conclusion With the increase of age,most of the syndromes of CID patients are disharmony between heart and kidney pattern,and there are obvious differences between the two types of sleep structure.Patients with disharmony between heart and kidney pattern have light sleep and low efficiency,while patients with liver qi stagnation pattern have many dreams.

Objective To explore the characteristics of polysomnography(PSG)and the types of Chinese medicine symptoms in elderly patients with chronic insomnia disorder(CID),and to provide reference for the prevention and diagnosis of CID in the elderly.Methods A total of 205 patients diagnosed as CID with parallel PSG in the outpatient clinic of the Department of Neurology of Zhejiang Provincial Tongde Hospital from May 2022 to May 2023 were selected for the study,and were divided into the geriatric group(≥ 60 years old)and the non-geriatric group(＜60 years old)based on the difference in age,and statistics were collected on the distribution of Chinese medicine syndromes in the patients of the two groups,and the PSG was used to record and compare the structure and process of sleep in the patients of the two groups.parameters,and do correlation analysis.Results Total sleep time(TST),sleep efficiency TST time in bed(TIB),total duration of rapid eyes movement(REM)period,total duration of N2 period,total duration of N3 period,and REM% were lower in elderly patients than in the non-elderly group(P＜0.05).And the time to wakefulness after sleep,N1 latency,N2 latency,and N1% were statistically higher than those of the non-elderly group(P＜0.05).The TCM diagnosis of elderly patients with CID is more commonly characterized by cardiac and renal disorders.Conclusion There were differences in polysomnographic sleep testing between the elderly and non-elderly groups of patients with CID.Compared with the non-elderly group,the elderly group had shorter sleep duration,lower sleep efficiency,longer sleep latency,reduced total REM duration,and the most common clinical Chinese medicine diagnostic typology of cardiac and renal disorders.

The typical hallmark of tumor evolution is metabolic dysregulation. In addition to secreting immunoregulatory metabolites, tumor cells and various immune cells display different metabolic pathways and plasticity. Harnessing the metabolic differences to reduce the tumor and immunosuppressive cells while enhancing the activity of positive immunoregulatory cells is a promising strategy. We develop a nanoplatform (CLCeMOF) based on cerium metal-organic framework (CeMOF) by lactate oxidase (LOX) modification and glutaminase inhibitor (CB839) loading. The cascade catalytic reactions induced by CLCeMOF generate reactive oxygen species "storm" to elicit immune responses. Meanwhile, LOX-mediated metabolite lactate exhaustion relieves the immunosuppressive tumor microenvironment, preparing the ground for intracellular regulation. Most noticeably, the immunometabolic checkpoint blockade therapy, as a result of glutamine antagonism, is exploited for overall cell mobilization. It is found that CLCeMOF inhibited glutamine metabolism-dependent cells (tumor cells, immunosuppressive cells, etc.), increased infiltration of dendritic cells, and especially reprogrammed CD8⁺ T lymphocytes with considerable metabolic flexibility toward a highly activated, long-lived, and memory-like phenotype. Such an idea intervenes both metabolite (lactate) and cellular metabolic pathway, which essentially alters overall cell fates toward the desired situation. Collectively, the metabolic intervention strategy is bound to break the evolutionary adaptability of tumors for reinforced immunotherapy.

Objective To prepare Compound Nanxing Pain Relief Gel(CNPRG)and evaluate its quality and sensitization.Methods CNPRG uses Carbopol 980 NF as the matrix;Appearance,viscosity,coating,centrifugal stability,cold stability,thermal stability as comprehensive indicators,single test and Box-Behnken effector method to optimize the prescription;The quality evaluation methods of appearance,pH,viscosity,stability,vapor phase identification of volatile components,and determination of diaconitine and eugenol content of CNPRG were preliminarily established;CNPRG sensitization was assessed by Active Cutaneous Anaphylaxis.Results The best prescription for CNPRG was Carbopol 980 NF 0.35 g,drug 1.02 g,glycerol 5.00 g,and pH 6.20.CNPRG ′s appearance likes jelly,is smooth,uniform and delicate;pH=6.20±0.03;viscosity 68.43±1.14 Pa·s;Centrifugation,high,low temperature stability,no stratification and precipitation;Identify camphor,(±)-Borneol and(±)-Isoborneol,Cinnamaldehyde,eugenol,phenol;Hypaconitine content 0.2983±0.0073 μg·g-1;Eugenol content 155.66±0.97 μg·g-1;CNPRG confirmed no sensitization.Conclusion CNPRG has good appearance,stable quality and no sensitization,and it can provide a choice for the development of new dosage forms of compound Nanxing pain relief cream to alleviate sensitization.

Objective To investigate the clinical efficacy of arthroscopic debridement combined with platelet rich plasma(PRP)in treatment of supraspinatus calcified tendinitis.Methods 23 patients in the experimental group were treated with shoulder arthroscopic calcification clearing with PRP injection,while 23 patients in the control group were treated with simple arthroscopic clearing,and the curative effects were observed and compared.Results All the patients were followed up effectively.Of the 23 cases in the experimental group,5 cases were treated with rotator cuff repair(3 cases were diagnosed rotator cuff tear before operation,2 cases were diagnosed with large calcification,and rotator cuff repair was performed after cleaning),and 3 cases were treated with rotator cuff repair(2 cases were diagnosed with rotator cuff tear before operation,1 case was diagnosed with large calcification,and rotator cuff repair was performed after cleaning).The visual analogue scale(VAS)of the two groups one month after surgery was significantly lower than that before surgery,and the American Association of Shoulder and Elbow Surgeons(ASES)score of the two groups one month after surgery was significantly higher than that before surgery,with statistical significance(P＜0.05).The VAS of the experimental group was significantly lower than that of the control group one month after surgery,and the ASES score was significantly higher than that of the control group one month after surgery,with statistically significant differences(t =-5.52,t = 8.73,P = 0.001).Conclusion Arthroscopic debridement combined with PRP is an effective and minimally invasive method for the treatment of supraspinatus calcified tendinitis.The calcium salt can be removed as much as possible under arthroscopy,and the scope of rotator cuff injury can be accurately evaluated.For the obvious rotator cuff injury with large damage,the rotator cuff repair with thread anchor nail can be performed.At the same time,combined with PRP,it can accelerate the repair of rotator cuff injury,relieve pain,and accelerate the functional recovery of shoulder joint.

In our country, the technology of extraction and separation of traditional Chinese medicine is backward relatively. The production process is extensive and the equipment level is low relatively. The manufacturing process is mainly based on unit operation and manual operation. The automatic control of the whole process has not been realized, which has seriously restricted the modernization of traditional Chinese medicine industry. If automatic control technology applied to traditional Chinese medicine in the extraction process, the process parameters of operation will be in the effective and strict monitoring and control, so as to improve the product quality and production efficiency, reduce costs, to achieve the green production. In this paper, the current situation and the application of new automation technology in the process of extraction traditional Chinese medicine in recent years were summarized, in order to provide a reference for Chinese medicine green and intelligent production.

BACKGROUND:Previous studies have confirmed that ethanol can promote adipogenic differentiation of bone marrow mesenchymal stem cells (BMSCs), and up-regulate the expression of PPARγ and aP2 in the tumor necrosis factor α (TNF-α) signaling pathway. As a member of the ZIP protein family, Zrt/Irt-like protein 1 (ZIP1) is closely related to bone metabolism and osteogenic differentiation.OBJECTIVE:To study the effect of BMSCs transfected by ZIP1 on TNF-α signaling pathway in the process of adipogenic differentiation.METHODS:The BMSCs from rabbits were isolated and cultured under different concentrations of alcohol (0.03, 0.09,0.15, 0.21 mol/L), followed by transfection by ZIP1 siRNA and ZIP1 expression vector.RESULTS AND CONCLUSION:After culture in alcohol, the expression levels of aP2 and PPARγ proteins were both significantly increased (P < 0.05), and the level of triglyceride was increased in all alcohol groups except for 0.03 mol/L alcohol group (P < 0.05). After siRNA transfection, the expression levels of aP2 and PPARγ as well as the level of triglyceride were increased significantly in all the alcohol groups (P < 0.05); however, ZIP1 transfection decreased the expression levels of aP2 and PPARγ proteins (P < 0.05). To conclude, ZIP1 siRNA could promote the adipogenic differentiation of BMSCs through the activation of TNF-α signaling pathway.

BACKGROUND: A silk fibroin/chitosan scaffold has good biocompatibility, osteoinductivity and degradability.OBJECTIVE: To review the structure, performance and application of the silk fibroin/chitosan scaffold in bone,cartilage and soft tissue engineering regeneration.METHODS: PubMed, Wanfang, and CNKI databases were retrieved by computer for articles related to the structure, performance and application of the silk fibroin/chitosan scaffold in orthopedics published from 1998 to 2016. The keywords were chitosan, silk protein, bone tissue engineering in English and Chinese, respectively.RESULTS AND CONCLUSION: The silk fibroin/chitosan scaffold is characterized by good biocompatibility, bone inductivity and biodegradability that make cells grow well on the scaffold. The silk fibroin/chitosan scaffold has been widely used in bone tissue engineering, and has a prominent performance in bone defect repair and cartilage injury treatment. Meanwhile, the silk fibroin/chitosan scaffold exerts a crucial role in wound healing as well as in the treatment of spinal nerve injury and other soft tissue injuries. However, the silk fibroin/chitosan scaffold currently is less reported in the clinical use due to various reasons, and it will be the main research direction of future efforts. As is known to us, silk protein can be used to prepare the cruciate ligament and construct tissue-engineered nuclei; therefore, the silk fibroin/chitosan scaffold can be developed in the treatment of tendon ligament injury and intervertebral disc tissue engineering.