OBJECTIVETo optimize the combination modes of medical materials in extract of Huanglian Jiedu decoction.

METHODDivided the medical materials of Huanglian Jiedu decotion into 15 groups and extracted by semi-bionic estraction (SBE), the contents of berberine, baicalin, geniposide, total alkaloid, total flavonoid, total integral calculus area of HPLC and extract of 1 000 were determined, standardized, weighted and evaluated, respectively.

RESULTThe maximum evaluated value Y for the extracting technique was AD + BC.

CONCLUSIONThe optimal combination mode of the perscription is that Rhizoma Coptidis and Fructus Gardeniae, Radix Scutellariae and Cortex Phellodendri Amurensis are decocted together respectively then the two decoctions are merged.

Coptis ; chemistry ; Dosage Forms ; Drugs, Chinese Herbal ; analysis ; isolation & purification ; Gardenia ; chemistry ; Phellodendron ; chemistry ; Scutellaria baicalensis ; chemistry

OBJECTIVETo investigate the effects of the solid-state fermentation technology in extracting the ingredients of traditional Chinese medicine (TCM).

METHODUsing Danggui Kushen pills as an example, four index ingredients of ferulic acid, matrine, matrine alkaloids and dry extract were compared comprehensively, and the difference between the two water extractions of fermented pills and unfermented pills was evalued.

RESULTThe comprehensive evaluated result was that extraction of fermented pills was better than that unfermented.

CONCLUSIONThe solid-state fermentation is conducive to the extraction of prescription of Danggui Kushen pills.

Chemical Fractionation ; methods ; Chromatography, High Pressure Liquid ; Drugs, Chinese Herbal ; chemistry ; isolation & purification ; metabolism ; Fermentation ; Reproducibility of Results ; Water ; chemistry

OBJECTIVETo optimize the extraction conditions for semi-bionic extraction of Guizhi Fuling pill decoction.

METHODThe best extraction condition of the semi-bionic extraction for Guizhi Fuling pill decoction was optimized by uniform design was optimized with paeoniflorin, cinnamic acid, laetrile, pachymic acid, total polysaccharose and dry extract as the indexes.

RESULTThe optimized extraction condition is that extracting for 3 times with solvent pH values of 4.98, 7.35 and 8.00, respectively, and the total extracting time was 4.99 h.

CONCLUSIONIn consideration of manufacturing fact, the pH value of thrice extractions is set at 5.0, 7.5, 8.0 respectively, and the extracting time is 2.0, 1.5, 1.5 h in turn.

Drugs, Chinese Herbal ; chemistry ; Hydrogen-Ion Concentration

OBJECTIVETo optimize the extraction condtions in the modification of Rhizoma Cyperi, which had been extracted by the technique of supercritical CO2 extraction.

METHODThe semi-bionic extraction (SBE) conditions were optimized through homogeneous design while the extracts (< or = 1 000), the total area of HPLC and dry extracts were adopted as markers.

RESULTThe optimized SBE extraction conditions are the following: pH in first extraction was adjusted to 2.005 3. And then pH in second and third extraction adjusted to 6.508 2 and 8.945 6, time of the whole extracted process was 3.912 7 h.

CONCLUSIONCombine the faction of production,we make sure the conclusion is pH in first extraction was 2.00, pH in second and third extraction adjusted to 6.50 and 9.00, duration of run was 2.0 h, 1.0 h and 1.0 h, respectively.

Carbon Dioxide ; chemistry ; Chromatography, Supercritical Fluid ; methods ; Cyperus ; chemistry ; Drugs, Chinese Herbal ; chemistry ; Hydrogen-Ion Concentration ; Rhizome ; chemistry

Traditional Chinese medicine(TCM) injection,which plays a unique role in the clinical treatment,is Chinese original dosage form.But in recent years due to its quality problems or unscientifically clinical use,some adverse reactions have reported,even several injections had serious adverse reactions.So how to develop TCM injection becomes one of the topics the public and scholars most concerned about.Therefore,the three "bottleneck" problems of TCM injection on the current development,which are the material basis and TCM theory,clinical efficacy and safety,and also quality control,were analyzed and discussed in the article.Then the countermeasures were proposed and provided ideas for future research and development of TCM injection.

Objective Comparing the serum fingerprint of four kinds of extract solution from Banxia Baizhu Tianma Decoction.Methods Taking rabbits as experiment animals,collecting blood,and separating serum after drenching semi-bionic enzyme extracting(SBEE) solution.HPLC was used to detect the fingerprints of medicated serum at different blood-collecting time in order to optimize the best blood collecting time,then the serum fingerprints of four kinds of solution collected were compared.Results HPLC showed that when blood collecting time was 2 h,the number and total area of chromatographic peak had the maximum value;SBEE Solution had the the maximum number and total area of chromatographic peak in the four kinds of solution at the best blood collecting time.Conclusion The component content in SBEE medicated serum is higher than that by other method.

WAE. Conclusion SBE method is better than the other three methods in the extraction of SFD components.