1.APOE-mediated suppression of the lncRNA MEG3 protects human cardiovascular cells from chronic inflammation.
Hongkai ZHAO ; Kuan YANG ; Yiyuan ZHANG ; Hongyu LI ; Qianzhao JI ; Zeming WU ; Shuai MA ; Si WANG ; Moshi SONG ; Guang-Hui LIU ; Qiang LIU ; Weiqi ZHANG ; Jing QU
Protein & Cell 2023;14(12):908-913
2.4E-BP1 counteracts human mesenchymal stem cell senescence via maintaining mitochondrial homeostasis.
Yifang HE ; Qianzhao JI ; Zeming WU ; Yusheng CAI ; Jian YIN ; Yiyuan ZHANG ; Sheng ZHANG ; Xiaoqian LIU ; Weiqi ZHANG ; Guang-Hui LIU ; Si WANG ; Moshi SONG ; Jing QU
Protein & Cell 2023;14(3):202-216
Although the mTOR-4E-BP1 signaling pathway is implicated in aging and aging-related disorders, the role of 4E-BP1 in regulating human stem cell homeostasis remains largely unknown. Here, we report that the expression of 4E-BP1 decreases along with the senescence of human mesenchymal stem cells (hMSCs). Genetic inactivation of 4E-BP1 in hMSCs compromises mitochondrial respiration, increases mitochondrial reactive oxygen species (ROS) production, and accelerates cellular senescence. Mechanistically, the absence of 4E-BP1 destabilizes proteins in mitochondrial respiration complexes, especially several key subunits of complex III including UQCRC2. Ectopic expression of 4E-BP1 attenuates mitochondrial abnormalities and alleviates cellular senescence in 4E-BP1-deficient hMSCs as well as in physiologically aged hMSCs. These f indings together demonstrate that 4E-BP1 functions as a geroprotector to mitigate human stem cell senescence and maintain mitochondrial homeostasis, particularly for the mitochondrial respiration complex III, thus providing a new potential target to counteract human stem cell senescence.
Mesenchymal Stem Cells/physiology*
;
Cellular Senescence
;
Homeostasis
;
Cell Cycle Proteins/metabolism*
;
Adaptor Proteins, Signal Transducing/metabolism*
;
Mitochondria/metabolism*
;
Electron Transport Complex III/metabolism*
;
Humans
;
Cells, Cultured
3.Exosomes from antler stem cells alleviate mesenchymal stem cell senescence and osteoarthritis.
Jinghui LEI ; Xiaoyu JIANG ; Wei LI ; Jie REN ; Datao WANG ; Zhejun JI ; Zeming WU ; Fang CHENG ; Yusheng CAI ; Zheng-Rong YU ; Juan Carlos Izpisua BELMONTE ; Chunyi LI ; Guang-Hui LIU ; Weiqi ZHANG ; Jing QU ; Si WANG
Protein & Cell 2022;13(3):220-226
4.Large-scale chemical screen identifies Gallic acid as a geroprotector for human stem cells.
Hezhen SHAN ; Lingling GENG ; Xiaoyu JIANG ; Moshi SONG ; Jianxun WANG ; Zunpeng LIU ; Xiao ZHUO ; Zeming WU ; Jianli HU ; Zhejun JI ; Si WANG ; Piu CHAN ; Jing QU ; Weiqi ZHANG ; Guang-Hui LIU
Protein & Cell 2022;13(7):532-539
5.Hyperthermia differentially affects specific human stem cells and their differentiated derivatives.
Si WANG ; Fang CHENG ; Qianzhao JI ; Moshi SONG ; Zeming WU ; Yiyuan ZHANG ; Zhejun JI ; Huyi FENG ; Juan Carlos Izpisua BELMONTE ; Qi ZHOU ; Jing QU ; Wei LI ; Guang-Hui LIU ; Weiqi ZHANG
Protein & Cell 2022;13(8):615-622
6.mTORC2/RICTOR exerts differential levels of metabolic control in human embryonic, mesenchymal and neural stem cells.
Qun CHU ; Feifei LIU ; Yifang HE ; Xiaoyu JIANG ; Yusheng CAI ; Zeming WU ; Kaowen YAN ; Lingling GENG ; Yichen ZHANG ; Huyi FENG ; Kaixin ZHOU ; Si WANG ; Weiqi ZHANG ; Guang-Hui LIU ; Shuai MA ; Jing QU ; Moshi SONG
Protein & Cell 2022;13(9):676-682
7.FTO stabilizes MIS12 and counteracts senescence.
Sheng ZHANG ; Zeming WU ; Yue SHI ; Si WANG ; Jie REN ; Zihui YU ; Daoyuan HUANG ; Kaowen YAN ; Yifang HE ; Xiaoqian LIU ; Qianzhao JI ; Beibei LIU ; Zunpeng LIU ; Jing QU ; Guang-Hui LIU ; Weimin CI ; Xiaoqun WANG ; Weiqi ZHANG
Protein & Cell 2022;13(12):954-960
8.Correction to: mTORC2/RICTOR exerts differential levels of metabolic control in human embryonic, mesenchymal and neural stem cells.
Qun CHU ; Feifei LIU ; Yifang HE ; Xiaoyu JIANG ; Yusheng CAI ; Zeming WU ; Kaowen YAN ; Lingling GENG ; Yichen ZHANG ; Huyi FENG ; Kaixin ZHOU ; Si WANG ; Weiqi ZHANG ; Guang-Hui LIU ; Shuai MA ; Jing QU ; Moshi SONG
Protein & Cell 2022;13(12):961-961
9.ALKBH1 deficiency leads to loss of homeostasis in human diploid somatic cells.
Hongyu LI ; Zeming WU ; Xiaoqian LIU ; Sheng ZHANG ; Qianzhao JI ; Xiaoyu JIANG ; Zunpeng LIU ; Si WANG ; Jing QU ; Weiqi ZHANG ; Moshi SONG ; Eli SONG ; Guang-Hui LIU
Protein & Cell 2020;11(9):688-695
10.SIRT7 antagonizes human stem cell aging as a heterochromatin stabilizer.
Shijia BI ; Zunpeng LIU ; Zeming WU ; Zehua WANG ; Xiaoqian LIU ; Si WANG ; Jie REN ; Yan YAO ; Weiqi ZHANG ; Moshi SONG ; Guang-Hui LIU ; Jing QU
Protein & Cell 2020;11(7):483-504
SIRT7, a sirtuin family member implicated in aging and disease, is a regulator of metabolism and stress responses. It remains elusive how human somatic stem cell populations might be impacted by SIRT7. Here, we found that SIRT7 expression declines during human mesenchymal stem cell (hMSC) aging and that SIRT7 deficiency accelerates senescence. Mechanistically, SIRT7 forms a complex with nuclear lamina proteins and heterochromatin proteins, thus maintaining the repressive state of heterochromatin at nuclear periphery. Accordingly, deficiency of SIRT7 results in loss of heterochromatin, de-repression of the LINE1 retrotransposon (LINE1), and activation of innate immune signaling via the cGAS-STING pathway. These aging-associated cellular defects were reversed by overexpression of heterochromatin proteins or treatment with a LINE1 targeted reverse-transcriptase inhibitor. Together, these findings highlight how SIRT7 safeguards chromatin architecture to control innate immune regulation and ensure geroprotection during stem cell aging.
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