OBJECTIVE To comprehensively evaluate the quality of Sanzi powder from different batches based on 12 components quantitative analysis combined with chemical pattern recognition and entropy weight-TOPSIS method. METHODS The contents of 12 components in 15 batches of Sanzi powder （No. S1-S15） were determined by HPLC-MS/MS， such as ethyl gallate， gallic acid， ferulic acid， corilagin， genipin-1-O-β-D-gentiobioside， toosendanin， geniposide， caffeic acid， methyl deacetylated coumarinate， tannic acid， rutin， quercetin. Cluster analysis （CA）， principal component analysis （PCA）， and orthogonal partial least squares-discriminant analysis （OPLS-DA） were conducted on the assay results. Using variable importance projection （VIP） value＞1 and P＜0.05 as the evaluation criteria， the quality differential markers in Sanzi powder were screened. The entropy weight method was used to calculate the weight value， and TOPSIS method was used to rank the quality of 15 batches of Sanzi powder from superior to inferior. RESULTS The contents of the 12 components were 13.494-24.292， 2 069.608-3 188.100， 1.410-3.616， 1 065.030-2 630.584， 1 404.704-1 838.078， 101.640-354.268， 9 193.720-14 777.854， 1.240-5.060， 148.028-5 541.990， 4 261.422-5 607.438， 107.560- 195.512， 2.226-4.192 μg/g， respectively. The results of CA， PCA and OPLS-DA indicated that 15 batches of Sanzi powder could be clustered into two groups. Specifically， batches S3， S7， S10 and S15 were grouped into one category， and remaining batches were grouped into one category. VIP values of geniposide， quercetin， caffeic acid， and methyl deacetylated coumarinate were all greater than 1， with corresponding P-values less than 0.05. The results of the entropy weight-TOPSIS analysis revealed that methyl deacetylate exhibited the smallest information entropy and the highest weight. The relative closeness degrees of samples S3， S7， S10 and S15 ranged from 0.789 to 0.973， while the remaining samples ranged from 0.054 to 0.172. CONCLUSIONS The contents of 12 components in Sanzi powder could be determined accurately by using HPLC-MS/MS technology. Methyl deacetylated coumarinate， geniposide， quercetin and caffeic acid were identified as the quality differential markers. It was found that the overall quality of samples S3， S7， S10 and S15 were superior to that of other batches. Notably， the quality of Gardeniae Fructus decoction pieces emerges as a critical factor in ensuring the consistency of the preparation’s quality.

A composite nystagmus classification framework enhanced by dual attention mechanism is proposed to address the problem that the existing researches only identify whether nystagmus occurs in a horizontal,vertical,or axial direction,but fail to consider the issue of composite nystagmus composed of multiple directions with various intensities in clinical practice.A spatiotemporal concentration algorithm for nystagmus videos is presented,and it combines convolutional neural networks and Hough transform to remove interference from invalid frames and regions and to improve the quality of nystagmus videos.Then,a dense optical flow algorithm is used to extract the optical flow field of eye movement.Finally,a composite nystagmus classification network based on dual attention mechanism enhancement is constructed.An improved efficient channel attention module is used to effectively obtain the direction and intensity of nystagmus in different channels of the optical flow map;and a temporal attention module is added at the end of the bidirectional long short-term memory network to achieve significant expression of classification results based on different temporal features.On the nystagmus dataset provided by the cooperating hospital,the proposed method has an accuracy rate of 83.17%for composite nystagmus classification,and achieved accuracy rates of 91.03%,89.74%,and 86.05%for individual horizontal,vertical,and axial nystagmus classifications.The proposed method realizes the intelligent classification of composite nystagmus and is valuable in clinic.

Objective:To investigate and analyze the allocation equality of occupational health technology service resource of Gansu disease control and prevention institutions, providing reference basis for optimizing the allocation of occupational health technology service resources.Methods:Combined with the survey data in September 2021, Gansu Province was divided into five economic regions by geographical location and economic characteristics. Taking the service number of enterprises and workers as the analysis dimensions, the allocation level of occupational health technology service resources in different regions was compared. The allocation equality was analyzed through Lorentz curve, Gini coefficient and Theil index.Results:There were 301 occupational health technicians and 1914 sampling and testing equipments of Gansu Provincial disease control and prevention institutions in 2021. All of the technicians and equipments were used for serving 1952 enterprises and 465800 workers. The curvature of Lorentz curves and Gini coefficient which measured by the service number of enterprises and workers were: occupational health technicians>radioactive factor sampling and testing equipment >physical factor sampling and testing equipment >chemical factor sampling and testing equipment, and chemical factor sampling and testing equipment>physical factor sampling and testing equipment >radioactive factor sampling and testing equipment >occupational health technicians, respectively. Theil index of occupational health technology service resources suggested that differences in regions were the main unfair reason effected the allocation of occupational health technicians and radioactive factor sampling and testing equipment, while the differences between regions were the main unfair reason effected the allocation of chemical and physical factor sampling and testing equipment.Conclusion:The allocation equality in occupational health technology service resources of Gansu Provincial disease control and prevention institutions was not enough, and the differences in regions and between regions should be considered. This study suggests that it is necessary to introduce more occupational health technicians. The allocation of occupational health technology service resources should match with the number of local enterprises and the types of potential hazard factors of enterprises as far as possible.

Objective:To explore the predictive effect of social support degree on the development trajectory of sleep disorder in patients with peritoneal dialysis after catheterization.Methods:A total of 281 patients who received peritoneal catheterization in Xinxiang Central Hospital from May 2021 to October 2022 were selected by the convenient sampling method. Sleep quality (PSQI) was assessed by using Pittsburgh Sleep Quality Index (PSQI) at the time of enrollment (T 0) and on day 3 (D 3) , day 7 (D 7) , day 14 (D 14) , day 21 (D 21) and day 28 (D 28) after peritoneal catheterization, and then the development trajectory of sleep disorders was analyzed by the latent class growth model. After assessed by Perceived Social Support Scale (PSSS) , patients were divided into low social support group and high social support group, and the differences in the development trajectory distribution of sleep disorders of patients were compared between two groups. Results:There were 134 patients (47.69%) in the low social support group and 147 patients (52.31%) in the high social support group. Three potential categories of sleep disorder development trajectories were identified, including 78 cases without sleep disorder (27.76%) , 79 cases with improved sleep disorder (28.11%) and 124 cases with persistent sleep disorder (44.13%) . The trajectories showed the lowest PSQI score at D 3, followed by D 7 and T 0. In the low social support group, there were 30 cases without sleep disorders (22.39%) , 34 cases with improved sleep disorders (25.37%) and 70 cases with persistent sleep disorders (52.24%) . In the high social support group, there were 48 cases without sleep disorders (32.65%) , 45 cases with improved sleep disorders (30.61%) and 54 cases with persistent sleep disorders (36.73%) . There was a statistically significant difference in the distribution of sleep disorder trajectories between the two groups of patients ( P<0.05) . Conclusions:Sleep disorders are common in patients undergoing peritoneal dialysis after catheterization, with an incidence rate of 72.24%, which is more obvious within one week after surgery. Patients with low social support have lower overall sleep quality and were more likely to have persistent sleep disorders.

Objective:To investigate and analyze the allocation equality of occupational health technology service resource of Gansu disease control and prevention institutions, providing reference basis for optimizing the allocation of occupational health technology service resources.Methods:Combined with the survey data in September 2021, Gansu Province was divided into five economic regions by geographical location and economic characteristics. Taking the service number of enterprises and workers as the analysis dimensions, the allocation level of occupational health technology service resources in different regions was compared. The allocation equality was analyzed through Lorentz curve, Gini coefficient and Theil index.Results:There were 301 occupational health technicians and 1914 sampling and testing equipments of Gansu Provincial disease control and prevention institutions in 2021. All of the technicians and equipments were used for serving 1952 enterprises and 465800 workers. The curvature of Lorentz curves and Gini coefficient which measured by the service number of enterprises and workers were: occupational health technicians>radioactive factor sampling and testing equipment >physical factor sampling and testing equipment >chemical factor sampling and testing equipment, and chemical factor sampling and testing equipment>physical factor sampling and testing equipment >radioactive factor sampling and testing equipment >occupational health technicians, respectively. Theil index of occupational health technology service resources suggested that differences in regions were the main unfair reason effected the allocation of occupational health technicians and radioactive factor sampling and testing equipment, while the differences between regions were the main unfair reason effected the allocation of chemical and physical factor sampling and testing equipment.Conclusion:The allocation equality in occupational health technology service resources of Gansu Provincial disease control and prevention institutions was not enough, and the differences in regions and between regions should be considered. This study suggests that it is necessary to introduce more occupational health technicians. The allocation of occupational health technology service resources should match with the number of local enterprises and the types of potential hazard factors of enterprises as far as possible.

In recent years, the management of respiratory diseases related to preterm birth has received extensive attention.In 2021, the American Thoracic Society brought together multidisciplinary experts in respiratory, neonato-logy, otolaryngology, sleep medicine, radiology and nursing specialties to develop Guidelines for outpatient respiratory management in infants, children, and adolescents with post-preterm respiratory disease (hereinafter referred to as the " Guideline" ), aiming to provide evidence-based medical evidence for standardized outpatient management of respiratory diseases associated with preterm birth at different ages.The Guideline was interpreted and summarized so that pediatric clinicians could correctly diagnose and treat these diseases, and understand and implement standardized outpatient management on the basis of evidence.

Objective:To understand the degree of professional autonomy of nurses in Pediatric Nursing Alliance and the status of pediatric nursing practice environment, which providing guidance for the development of a series of specialized training in the alliance.Methods:Stratified random sampling method was used to conduct a questionnaire survey on nursing staff of different professional levels in Pediatric Nursing Alliance, which through the questionnaire star by using the questionnaire general information and training demand questionnaire, nurses practice professional autonomy scale, pediatric nursing staff structural empowerment questionnaire and nursing practice influencing factors questionnaire through the questionnaire star.Results:The total score for professional autonomy of nurses in the pediatric alliance was 192.66±18.63, the structural empowerment ( OR=1.137, 95% CI=1.084-1.194), lack of caring team ( OR=2.763, 95% CI=1.443-5.292) and performance evaluation ( OR=0.498, 95% CI= 0.274-0.908), specialized education and professional experience ( OR=0.548, 95% CI= 0.334-0.871) were affecting the clinical nursing practice. Conclusion:The degree of professional autonomy of nurses in the Pediatric Nursing Alliance is in the middle and high level. Key factors affecting nursing practice including insufficient structural empowerment, lack of opportunities to continue learning, lack of nursing teams, lack of effectiveness evaluation and the lack of specialized education and work experience, which guiding the pediatric nursing alliance to continuously deepen the connotation of pediatric nursing professional and innovative team collaboration new model, utilize the advantages of resources to actively cultivate specialized nursing research personnel, carry out multi-disciplinary and cross-disciplinary cooperation, improve the nursing quality evaluation index system, so as to enhance the professional nursing service capacity and value.

Aromatic L-amino acid decarboxylase deficiency(AADCD) is a rare early-onset autosomal recessive genetic neurometabolic disease which can be treated with target therapy.To find, diagnose and treat at its early stage is important to improving the prognosis.The international neurotransmitter-related diseases working group (iNTD) developed the consensus guidelines for the diagnosis and treatment of AADCD in 2017, which provided reco-mmendations based on the consensus to facilitate the diagnosis, treatment and long-term management of patients.This article interpreted and summarized the clinical symptoms and treatment of AADCD according to the consensus, in order to raise pediatric clinicians′ awareness and treatment level of the disease.

Objective:To study the different region (DFR) genotyping of 15 strains of Yersinia pestis from Inner Mongolia. Methods:Fifteen strains of Yersinia pestis isolated from Otog Banner and Darhan Muminggan Joint Banner in Meriones unguiculatus plague foci in Inner Mongolia Plateau, Zhengxiangbai Banner in Spermophilus dauricus plague foci in the Songliao Plain in 2019 were amplified by PCR, and identified DFR genotyping. Results:Two strains of Yersinia pestis in Otog Banner jointly deleted DFR loci 1, 6, 7, 13, 15, 16, 17, 18, 23, and 13 strains of Yersinia pestis in Darhan Muminggan Joint Banner and Zhengxiangbai Banner jointly deleted DFR loci 1, 6, 7, 12, 13, 15, 16, 17, 18 and 23. G17 and G20 were classified as genotyping by comparison with DFR locus distribution and genotyping results. Conclusion:The genotyping of Yersinia pestis in the natural foci of Meriones unguiculatus plague foci in Inner Mongolia Plateau and Spermophilus dauricus plague foci in the Songliao Plain is the same as that of the historical strain.

Objective:A strain of Yersinia pestis phage was isolated from the cecum of Meriones unguiculatus, and its biological characteristics were studied. Methods:The phage was isolated from the cecum of a Meriones unguiculatus which died in the Meriones unguiculatus natural foci of plague in Inner Mongolia. Phage morphology was observed by transmission electron microscopy (TEM). The optimal multiplicity of infection (MOI), one-step growth curve, stability of the phage to temperature and acidity and alkalinity, sensitivity to chloroform, ethanol, isopropanol and ultraviolet rays, host range of phage lysis and lysis of the phage to wild strains of Yersinia pestis were analyzed. Results:The diameter of the phage plaque was about 10.00 mm. The head of the phage showed icosahedral symmetry under TEM, and the head was composed of a head with a diameter of 51.72 nm and a tail with a length of 10.00 nm. The MOI was 0.01, the incubation period was about 20 min, the outbreak period was 20-100 min and the outbreak amount was 200 PFU/cell. Under the condition of pH 5-12, it could maintain high activity, not sensitive to chloroform, 10% ethanol and isopropanol. Ultraviolet irradiation for 2 min could kill most of the phages. The host spectrum of the phage was narrow, and with strong specificity. It could lyse wild strains of Yersinia pestis exclusively. Conclusions:The phage is classified as family Podoviridae, which has a potential to assist the diagnosis of Yersinia pestis, enriches the database of Yersinia pestis phages and lays a foundation for the research and application of Yersinia pestis phages.