Objective:To investigate the impact of peak pressure upon different bone densities during autonomous puncture by orthopedic robot on puncture path deviation and bone cement leakage.Methods:A spinal surgery robot system was designed for autonomous vertebral puncture and bone cement injection, and six porcine spine specimens were used for single-segment or double-segment simulated percutaneous vertebral augmentation surgery. The accuracy of puncture path (Gertzbein-Robbins grading), bone cement leakage classification, and peak bone drill pressure were measured to assess the accuracy of autonomous vertebral puncture and bone cement leakage in vertebral cortical and cancellous bone of different densities.Results:A total of 64 porcine vertebrae were simulated for puncture, among which 53 vertebrae were classified as Grade A, 8 as Grade B, and 3 as Grade C according to the Gertzbein-Robbins grading. The cortical bone pressure of Grade A vertebrae was 6.663±0.319 N which was lower than that of Grade B (8.348±0.418 N) and Grade C (11.500±0.600 N), with significant differences ( F=341.000, P<0.001). The cancellous bone pressure of Grade A, B, and C vertebrae were 3.660±0.317, 3.594±0.608, and 4.117±0.257 N, respectively, with no significant difference ( F=2.496, P=0.091). There were 40 cases of no leakage, 20 cases of Type I leakage (leakage into the surrounding vertebrae), and 3 cases of Type II leakage (leakage into the vertebral canal), with an overall leakage rate of 36% (23/64). The peak cortical bone pressure for no leakage, Type I, and Type II leakage was 6.638±0.301, 6.792±0.404, and 6.753±0.473 N, respectively, and the peak cancellous bone pressure was 3.634±0.279, 3.783±0.423, and 3.920±0.255 N, respectively, with no significant difference ( F=1.521, P=0.227; F=2.106, P=0.131). Conclusion:During the autonomous puncture process of the novel orthopedic robot, the accuracy of autonomous puncture path decreased when the puncture pressure through the cortical bone was high, and the probability of invading the pedicle increased. The puncture pressure of cortical and cancellous bone had no significant effect on the occurrence rate of bone cement leakage.

[Objective]To investigate the biological basis of the theory of"heart corresponding to summer"by observing the differences in the secretion of atrial natriuretic peptide(ANP),brain natriuretic peptide(BNP)and amino-terminal pro-B-type natriuretic peptide(NT-proBNP)in the myocardial tissues of the rats at the summer solstice and the winter solstice.[Methods]Specific pathogen free grade male SD rats were randomly divided into 4 groups,which were treated as normal control group,chronic heart failure(CHF)group,pineal gland removal group and composite model(CHF+pineal gland removal)group,and the contents of ANP,BNP and NT-proBNP were detected in myocardial tissues of the rats in each group at the winter solstice and summer solstice time points.[Results]Under normal physiological conditions,the secretion of ANP,BNP and NT-proBNP in rat myocardial tissues showed seasonal rhythmic changes of low in summer and high in winter;after the removal of the pineal gland,the seasonal rhythmic differences of ANP,BNP and NT-proBNP disappeared,and even appeared to be inverted;under the condition of CHF,the secretion of ANP,BNP and NT-proBNP in rat myocardial tissues showed seasonal rhythmic changes of high in summer and low in winter,and the original rhythm disappeared after the removal of the pineal gland.[Conclusion]Myocardial tissue ANP,BNP,NT-proBNP is the important biological material basis of the"heart corresponding to summer",their secretion of summer and winter seasonal rhythm changes,and the pathological state of CHF show the opposite of the normal state of the seasonal rhythm.At the same time,the pineal gland is involved in the seasonal regulation of the heart corresponding to summer.

ObjectiveTo study the brain network during balance control tasks in older adults. MethodsFrom January to April, 2022, 22 healthy young adults and 20 healthy older adults were recruited from the Fifth Affiliated Hospital of Guangzhou Medical University and communities. They were asked to finish standing tasks on the plantar pressure plate with eyes opening and closing, while the functional connectivities (FC) of prefrontal cortex (PFC) and primary motor cortex (PMC) were measured with functional near-infrared spectroscopy. ResultsCompared with the young adults, the area of the ellipse (Z = -2.884, P < 0.01) and the maximum swing (Z = -2.481, P < 0.05) increased in the older adults as eyes closing. During the standing task, the intra-FC of left (t = 2.978, P < 0.01) and right (Z = -3.123, P < 0.01) PFC decreased in the older adults, and the inter-FC of right PMC to left PFC (t = 2.087, P < 0.05) and right PFC to left PFC (t = 3.471, P < 0.001) decreased, too. ConclusionThe FC of PFC decreased in healthy older adults during balance control tasks, which may be a indicator for aging brain.

Humans ; Pyriform Sinus/surgery* ; Thyroid Neoplasms/diagnosis* ; Fistula/surgery* ; Diagnostic Errors ; Pharyngeal Diseases/surgery*

Objective:To investigate the effect of smart air cell mattresses on sleep quality.Methods:Twenty healthy young people were enrolled as subjects, and each subject underwent a four-night polysomnographic monitoring experiment, including two nights each on a smart air cell mattress and a general mattress. The differences in sleep quality were compared by self-assessment of sleep quality, objective sleep indicators, and electroencephalogram (EEG) spectral analysis.Results:In the comparison between the smart air cell mattress and the general mattress, the differences in self-assessment of sleep quality and objective sleep indicators were not statistically significant (all P > 0.05), but the smart air cell mattress had a slight overall advantage. The relative power of EEG in the low-frequency band and the relative power of EEG in the high-frequency band were higher in the subjects with the smart air cell mattress. Conclusions:For the healthy young population, the smart air cell mattress can positively influence sleep quality to some extent, and the change in EEG relative power indicates an increase in sleep depth.

The clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9 system has been widely used for genome engineering and transcriptional regulation in many different organisms. Current CRISPR-activation (CRISPRa) platforms often require multiple components because of inefficient transcriptional activation. Here, we fused different phase-separation proteins to dCas9-VPR (dCas9-VP64-P65-RTA) and observed robust increases in transcriptional activation efficiency. Notably, human NUP98 (nucleoporin 98) and FUS (fused in sarcoma) IDR domains were best at enhancing dCas9-VPR activity, with dCas9-VPR-FUS IDR (VPRF) outperforming the other CRISPRa systems tested in this study in both activation efficiency and system simplicity. dCas9-VPRF overcomes the target strand bias and widens gRNA designing windows without affecting the off-target effect of dCas9-VPR. These findings demonstrate the feasibility of using phase-separation proteins to assist in the regulation of gene expression and support the broad appeal of the dCas9-VPRF system in basic and clinical applications.
Humans ; Transcriptional Activation ; RNA, Guide, CRISPR-Cas Systems ; Gene Expression Regulation ; CRISPR-Cas Systems/genetics*

To evaluate the safety of the domestic 13-valent pneumococcal polysaccharide conjugate vaccine-tetanus toxoid protein (PCV13-TT) after its licensure. The adverse event following immunization (AEFI) and the vaccination data of PCV13-TT in Zhejiang province from July 2020 to October 2021 were collected from national adverse event following immunization surveillance system and Zhejiang provincial immunization information system. Descriptive epidemiological method was used for this analysis. From July 2020 to October 2021, 302 317 doses of PCV13-TT were administered in children under 6 years old in Zhejiang Province and 636 AEFI case reports were received, with a reporting rate of 21.04 per 10 000 doses. Of these AEFI cases, 97.17% were mild vaccine product-related reaction (20.54 per 10 000 doses) and 95.44% occurred in the 0-1 d after vaccination (20.08 per 10 000 doses). The most common clinical diagnoses of AEFI included fever (224 cases), redness (204 cases), and induration (190 cases), while allergic rash (11 cases) was the most common diagnosis among the abnormal reactions. In conclusion,the present results bolstered that the domestic PCV13-TT was generally well tolerated in children under 6 years old in Zhejiang Province.
Child ; Humans ; Child, Preschool ; Vaccines, Conjugate/adverse effects* ; Pneumococcal Vaccines/adverse effects* ; Vaccination ; Immunization ; Polysaccharides

Objective To compare and analyze the efficacy of PVA particles and Embosphere microspheres for partial splenic embolization (PSE)in the treatment of hypersplenism secondary to liver cirrhosis.Methods Sixty patients underwent PSE for treatment of hypersplenism secondary to liver cirrhosis were analyzed retrospectively.According to different PSE embolization materials,they were divided into two groups:30 patients with PVA particles embolization (group A),and other 30 patients with Embosphere microspheres embolization (group B).The changes of white blood cells (WBC),platelets (PLT),spleen thickness,portal vein diameter and postoperative pain and fever were compared on the 3rd day,the 1st week,the 3rd week,the 1st month,the 3rd month,and the 6th month after PSE and compared between the two groups.Results The WBC and PLT of the 2 groups after PSE were significantly higher than those before PSE (all P＜0.05 ).The spleen shrank after 2 months,and there was no significant difference in spleen thickness and portal vein diameter between the two groups at the same time (all P＞0.05).The postoperative pain in group A was significantly less than that in group B (P＜0.05).Conclusion Both PVA particles and Embosphere microspheres have good curative effect in the treatment of hypersplenism secondary to liver cirrhosis. The price of PVA particles is lower than that of Embosphere microspheres,and the postoperative pain degree is lighter than that of Embosphere microsphere,which suggests PVA particles with more advantages in clinical application.

OBJECTIVETo explore the acupoint electrosensitivity and regularity for assisting the clinical diagnosis and treatment so as to improve the clinical therapeutic effects.

METHODSBy means of the computer retrieval (CBMdisc, CNKI, VIP, WanFang, PubMed and EMBASE) and manual retrieval, the relevant literature on acupoint electrosensitivity was reviewed and the modern literature database of acupoint electrosensitivity was established. Using bibliometric, the relations were analyzed among the detected indexes, acupoints, meridians and disorders in the conditions of acupoint electrosensitivity, and the potential regularity was explored.

RESULTSThe general detection indexes of acupoint electrosensitivity were resistance, inertia area, volt-ampere area, electric conduction quantity, electric potential, electric current and capacitance. The electrosensitive points included meridian points, auricular points and extraordinary points. Among those points, the electrosensitivity easily occurred at the special points.

CONCLUSIONThe acupoint electrosensitivity is mainly reflected on the electrical impedance and volt-ampere characteristics. The relative specificity exists between acupoint electrosensitivity and disorders. In clinical practice, detecting acupoint electrosensitivity may assist the diagnosis and instruct acupoint selection to improve the clinical therapeutic effects.

Five main flavonoids of Hebei Xiangju were studied using the Density Functional Theory (DFT) B3LYP method with 6-311 G (d) basis set．Their activities were analyzed based on molecular structure,bond dissociation energy (BDE),natural orbital charge distribution (NBO),bond order and the energy gap between HOMO and LUMO. The results showed that the existing of intra molecular hydrogen bond in B ring can improve the antioxidant activity of the flavonoids, at the same time, the hydroxyl groups on the glycosides do not have the activity of eliminating free radicals, but decrease the total molecular antioxidant activity. As a result, the antioxidant ability order of the five flavonoids compounds is luteolin< luteolin-7-O-glucoside< apigenin < acacetin < acacetin-7-O-glucose, which is agreement with the experimental conclusion reported in literature. The results showed that the DFT method can provide theoretical guidance for the selection of natural flavonoid antioxidants.