Objective To investigate the occupational health status of the radiation workers in Xiamen, China, and provide direction and basis for improvement of occupational health surveillance. Methods After excluding duplicate records, a total of 7000 individuals who underwent occupational health examinations due to radiation exposure in Xiamen City from 2022 to 2023 were selected. Among them, 2499 individuals underwent examinations before employment, 4090 during employment, and 411 at the time of job separation. The results were analyzed. Results A total of 6979 (99.7%) individuals were deemed fit to engage (or continue engaging) in radiation work or to leave the position, while 21 (0.3%) individuals were found unfit. In the routine medical examinations of different genders, the items with relatively high abnormal rates included overweight and obesity (35.22%, χ² = 43.29, P < 0.01), abnormal liver function (33.74%, χ² = 153.53, P < 0.01), abnormal renal function (25.83%, χ² = 195.22, P < 0.01), and hyperuricemia (24.53%, χ² = 267.78, P < 0.01). For all of these items, the abnormality rates were significantly higher in males than in females. The abnormal rates of examination items related to radiation hazard factors were generally higher for females than for males. Statistically significant gender differences were observed in lens abnormality rate (χ² = 11.38, P < 0.001), the decrease rate of white blood cells (χ² = 12.04, P < 0.001), the abnormal rate of red blood cells (χ² = 25.47, P < 0.01), the abnormal rate of platelets (χ² = 88.07, P < 0.01), and the micronucleus rate of peripheral blood lymphocytes (χ² = 6.98，P = 0.008). The difference in micronucleus rate of peripheral blood lymphocytes among groups with different years of radiation exposure was statistically significant, and the rate increased with increasing exposure years. Conclusion Long-term exposure to radiation has a certain impact on the health of radiation workers. Radiation workers should enhance radiation protection measures, increase the awareness of protection, and reduce radiation-related damage.

Laboratory medicine is an important subject of auxiliary clinical diagnosis, and the postgraduate education of clinical laboratory diagnostics is the key to cultivating high-end laboratory talents. This study analyses the differences in the postgraduate education model of clinical laboratory diagnostics between China and Australia from the four aspects of academic degree system, curriculum setting, teaching methods, and autonomous learning ability training and discusses the postgraduate education reform of laboratory medicine in China. With reference to the advanced experience of countries with a well-developed education system, the education reform in China can improve the comprehensive quality of postgraduate student in laboratory medicine and enhance the medical level of China.

Objective To develop an oxybutynin nanotransdermal formulation with high skin permeability based on cubsomes carrier.Methods The oxybutynin loaded cubsomes(OXY-CS)was prepared by ultrasonic method.Using particle size,polydispersity index and encapsulation efficiency as evaluation indexes,stirring temperature,stirring time,stirring speed,ultrasound time,ultrasound power,glycerol monooleate/poloxamer 407 ratio(GMO/F127),carrier(GMO+F127)/drug ratio and drug concentration were optimized.The effects of drug concentration,permeation promoter and gel matrix type on skin permeability were investigated using cumulative permeation and skin retention as evaluation indicators.Results The prepared OXY-CS had small particle size and high encapsulation efficiency at stirring temperature of 50℃,stirring time of 30 min,stirring speed of 1 000 r/min,ultrasound time of 10 min,ultrasound power of 100 W,GMO/F127 ratio of 2∶1,carrier/drug ratio of 3∶1 and drug concentration of 1％,respectively.OXY-CS had high cumulative penetration and skin retention at concentration ≥ 3％.The addition of a permeation promoter to OXY-CS did not increase the cumulative penetration of the drug.The skin permeability of oxybutynin cubsomes gel made with carbomer was significantly better than those of other gel matrices.Conclusion In this study,oxybutynin is made into a topical cubsomes gel,which is expected to reduce the incidence of adverse effects associated with oral administration.

Objective:To construct a eukaryotic expression vector for bacteriophage D29 LysinB/Holin fusion protein and study its bactericidal efficacy against Mycobacterium tuberculosis ( Mtb) in a cell infection model. Methods:A recombinant plasmid pET32a-LysinB was constructed and induced to express LysinB. The polyclonal antibody against LysinB was prepared after the purification of LysinB. A recombinant plasmid pcDNA3.1(+ )-LysinB/Holin was constructed and transfected into mononuclear macrophages RAW264.7. After the expression of the prepared polyclonal antibody was identified, a cell infection model was established and the bactericidal efficacy of LysinB/Holin fusion protein was measured by acid-fast staining and colony counting.Results:The polyclonal antibody against LysinB was successfully prepared. The recombinant plasmid pcDNA3.1(+ )-LysinB/Holin could effectively express LysinB/Holin fusion protein in eukaryotic cells without inducing significant cytotoxicity. LysinB/Holin fusion protein was effective in killing Mtb in cells. Conclusions:The recombinant plasmid pcDNA3.1(+ )-LysinB/Holin has a better killing effect on intracellular Mtb without inducing obvious cytotoxicity against eukaryotic cells, showing a potential in the treatment of tuberculosis.

This paper takes the teaching of pathogeny biology and clinical laboratory diagnostics for postgraduates in Shandong Second Medical University as examples. By constructing innovative thinking in scientific research, training research innovation skills, and establishing modular, multi-evaluation systems, a comprehensive training mode for scientific research and innovation ability of medical academic postgraduate students had been proposed, and the implementation and effects of this training mode were thoroughly explored. This mode is expected to bring a positive impact on the training of medical postgraduate students and improve their scientific research and innovation ability, thereby promoting the cultivation of more medical talents with a high degree of scientific research and innovation ability.

Currently, the teaching work for postgraduates mainly centers on specific disciplines, and there is a lack of effective connection between disciplines, which leads to the insufficient ability of postgraduates in effectively integrating and applying the knowledge of various disciplines and restricts the development of innovation ability. This paper proposes a program for the cultivation of innovation ability of postgraduate students in clinical laboratory diagnostics based on multi-disciplinary integration from the three aspects of curriculum setting, multi-disciplinary interactive platform construction, and tutor team construction, aiming at helping postgraduate students to enhance the ability of knowledge integration and application and form a comprehensive disciplinary perspective. Additionally, the paper explores the practical application and effect of the cultivation program, offering novel insights and approaches for talent cultivation in the realm of clinical laboratory diagnostics.

Weifang Medical University has taken the lead in setting up laboratory skills courses to train talents in Shandong Province, and held test skills competitions every year to test the effectiveness of learning. Based on the course of testing skills, medical humanistic literacy has been cultivated, and the basic knowledge and basic operation have been learned; taken the skill contest as the medium, the learning results have been tested and the professional skills have been strengthened. Through the combination of test skill course and test skill competition to cultivate applied test talents adapted to the times, it can meet the needs of all kinds of related posts.

The undergraduate four-year medical laboratory technology major aims to cultivate advanced medical application-oriented talents. The realization of this purpose depends on the construction of an innovative practical teaching curriculum system. This article combines the four modules of basic practice, professional practice, vocational skill practice, and innovative practice to improve the problems existing in the traditional practice teaching system, and proposes a "3+1" three-dimensional practice teaching model in order to adapt to the rapid development of medical laboratory technology.

Apoptosis is particularly important for the body to inhibit or eliminate Mycobacterium tuberculosis ( Mtb) infection. A series of mechanisms to regulate macrophage apoptosis are triggered after macrophages are infected by Mtb. In Mtb-infected macrophages, there are differentially expressed microRNAs (miRNAs), which can directly bind to the binding sites in the 3′-untranslated region (3′ UTR) of apoptotic genes to regulate the expression of apoptotic genes and the apoptosis of macrophages through the mitochondrial or death receptor pathway. This paper reviewed the miRNAs associated with the apoptosis of Mtb-infected macrophages and the major mechanisms.

OBJECTIVE: To analyze the characteristics of cervical microecology in late reproductive-age women with cervical lesions and explore new methods for preventing cervical lesions. METHODS: Cervical smears were obtained from a total of 147 women of late reproductive age, including 24 with high-risk HPV infection (HR-HPV), 27 with low-grade squamous intra-epithelial lesions (LSIL), 36 with high-grade squamous intra-epithelial lesions (HSIL), 35 with cervical cancer (CC) and 25 healthy women. llumina MiSeq sequencing of V3-V4 region of the 16S rRNA gene amplicons was used to characterize the vaginal microbiota of the women. OTUs analysis of the valid data was performed, and the α-diversity (Chao1, Simpson's Index and Shannon Index) and β-diversity (T-test, weighted UniFrac β diversity, and MetaStat analysis) were evaluated. RESULTS: Dilution curve and species accumulation boxplot validated the quality of the samples. OTUs analysis of the 5 groups demonstrated that cervical bacterial genus consisted primarily of CONCLUSIONS The abundance of
Female ; Humans ; Microbiota ; Papillomaviridae ; Papillomavirus Infections ; RNA, Ribosomal, 16S/genetics* ; Uterine Cervical Neoplasms ; Vaginal Smears