Objective: To study the effects of long non-coding RNA linc-01135 on the osteogenic differentiation of inflammatory PDLSCs(P-PDLSCs) under 12％ static mechanical strain loading. Methods: Cells were isolated and cultured from the healthy and periodontitis samples respectively to obtain healthy PDLSCs(H-PDLSCs) and P-PDLSCs. RT-PCR were used to identify the expression level of linc-01135. Lentiviruses were used to upregulate and downregulate the expression of linc-01135, and the osteogenesis gene expression were analyzed by RT-PCR and the osteogenesis differentiation capability were evaluated by alizarin red staining. Results: linc-01135 expression in P-PDLSCs was lower than that in H-PDLSCs. When the expression of linc-01135 was upregulated in P-PDLSCs before 12％ SMS loading, the expression level of RUNX2, ALP, OPG was significantly increased. In contrast, the expression of RUNX2, ALP, OPG was significantly decreased when the expression of linc-01135 was suppressed. Alizarin red staining proved the same trend. Conclusion: linc-01135 can promote the osteogenic differentiation capability of P-PDLSCs under 12％SMS loading.

Objective To investigate the impact of transtheoretical model-based depression management on senile people with type 2 diabetes. Methods A total of 78 senile patients with type 2 diabetes were recruited from the Affiliated Jiangyin Hospital of Southeast University Medical College and the Nantong Tumor Hospital during the period from September 2015 to December 2016.They were randomly divided into the experimental(n=39)and the control groups(n=39).The patients in the experimental group received transtheoretical model-based depression management while those in the control group received traditional health education. The intervention results were evaluated using Demographic Data Recording Form, Self-Rating Depression Scale, and Depression Prevention & Management Survey Items. The main statistical methods included Chi square test,independent sample t test,Mann-Whitney U test,and repeated measures analysis of variance. Results After the intervention of three months, the mean scores of Self-Rating Depression Scale was 50.97 (F=17.814), perceived benefit score was (9.59 ± 2.10) (t=-6.732), perceived barriers score was (5.59 ± 1.30) (t=2.980), cognitive level score was (32.98 ± 4.87) (t=-10.189), the behavior level score was (34.32 ± 5.02) (t=-13.745), and the self-efficacy score was (12.11 ± 2.13)(t=-7.845),all of which significantly improved in the experimental group,more than the control group with its perceived benefit score of(8.31 ± 1.12)(F=32.789),perceived barriers score of(6.99 ± 2.12)(F=18.788), cognitive level score of (29.01 ± 3.34) (F=67.876), behavior level score of (28.03 ± 4.77) (F=43.476), and self-efficacy score of(9.78 ± 2.03)(F=15.543),and the difference was statistically significant(P<0.01).The level of FPG in experimental group was(7.24 ± 1.36)mmol/L,which was significantly lower than the FPG of (7.41 ± 1.34)mmol/L in the control group(t=0.624,P=0.028).Conclusions Transtheoretical model-based depression management can effectively improve senile type 2 diabetes'management of depression,which is valuable in the clinical context.

Objective To understand the status and influencing factors of benefit finding in patients with cancer.Methods 350 patients with cancer were collected with convenience sampling method from two Jiangsu hospitals.All patients received a questionnaire survey.Multiple linear regression analysis was used to analyze the influencing factors of benefit finding in patients with cancer.Results (1) The score of benefit finding in patients with cancer was statistically significant in different frequency of physical exercise (F=8.37,P=0.00),different character (F=3.04,P=0.04) and different educational level (F=2.91,P=0.03).(2) There was a positive correlation between benefit finding(78.53± 10.50) and communication with physicians(7.27±2.19),medical coping(43.48±4.50),optimism(17.33±2.24) and social support(44.81±3.91)(r=0.31,0.35,0.29,0.23,all P＜0.05) in patients with cancer.The benefit finding in patients with cancer was negatively correlated with depression(8.59± 1.83) (r=-0.37,P＜0.05).(3) Multiple linear regression analysis results showed that medical coping modes-confrontation,objectively social support and utilization,physical exercise,depression,communication with physicians and character were the factors influencing benefit finding in patients with cancer(△R2 =28％,P＜ 0.05;△R2 =5.5％,P＜ 0.05;△R2 =3.1％,P＜ 0.05;△R2 =1.9％,P＜0.05;△R2 =1.7％,P＜0.05;△R2 =1.0％,P＜0.05;△R2 =1.1％ P＜0.05),which explained 42.0％ of the total variance.Conclusion Medical coping modes-confrontation,objectively social support and utilization,physical exercise,depression,communication with physicians and character are the factors influencing benefit finding in patients with cancer.

Objective To observe the dynamic histological changes of condylar cartilage ,and detect expression of estrogen re‐ceptors and collagen typeⅡin the ovariectomized rat ,exploring the relationship between OA and abnormity of shorting estrogen . Methods Histological changes of condylar cartilage of the ovariectomized rat at different ages were observed by HE staining meth‐od .The expression of estrogen receptors and collagen typeⅡwere determined by immunohistochemical method ,and the average per‐centage of positive staining area was measured by image analyzer .Results The lack of estrogen led to the degeneration of condylar cartilage .The expression of ER and ColⅡ were suppressed at low concentration of estrogen ,and these effects were enhanced when the treating time was prolonged .Conclusion The lack of estrogen might lead to the rat′s condylar cartilage lesion ,and the low con‐centration of estrogen could suppress the expression of ER .These results highlight the results that expression of condylar cartilage collagen is associated with estrogen .

Objective To investigate the effects of predictive nursing intervention on acute myocardial infarction ( AMI ) patients undergoing temporary preventive cardiac pace maker .Methods A total of 130 patients with AMI undergoing emergency percutaneous coronary intervention ( PCI) were enrolled by convenience sampling method and divided into control group (n=65) and observation group(n=65) according to the time of admittion.All patients were received temporary preventive cardiac pace maker before PCI .Routine nursing was applied in patients of the control group , and predictive nursing was applied in patients of the observation group . Malignant arrhythmia , hypotension and serious complications were recorded and analyzed .Results In both groups, the right coronary artery was the main artery of the infarction , accounting for 80.00%(control group, 52/65), 76.92%(observation group, 50/65) respectively, the difference was not statistically significant (P>0.05).Malignant arrhythmia rate in the observation group tended to decrease compared with that of the control group (7.69% vs 18.46%), but there′s no statistically significant difference ( P =0.069 ).Malignant arrhythmia rate of right coronary artery infarction in the observation group was lower than that of the control group (6.00%vs 21.15%) ( P =0.026 ).12.31% of observation group patients developed hypotension versus 26.15% in the control group ( P =0.045 ).Predictive nursing intervention had a tendency to reduce complications occurrence compared with routine nursing intervention (10.77%vs 20.00%), but no statistical significance was achieved(P=0.145).Conclusions Implementation of predictive nursing intervention among AMI patients undergoing temporary preventive cardiac pace maker plays an important role in reducing the risks of malignant arrhythmia and hypotension , which has certain meaning for the clinical nursing .

BACKGROUND:How to effectively improve the airway management and improve oxygen supply at the same time through a simple and effective method becomes an operation key for physicians in fiberoptic bronchoscopy. OBJECTIVE:To observe the clinical effect of self-made simple masks in painless fiberoptic bronchoscopy. METHODS:A total of 60 patients receiving painless fiberoptic bronchoscopy, 32 males and 28 females, with a mean age of (57.8±10.9) years were randomized into two groups: test group with self-made simple mask during painless fiberoptic bronchoscopy and control group with laryngeal mask. In the course of examination, the changes of oxygen saturation, mean arterial pressure and heart rate were observed at different time points. And adverse events of both groups were also recorded. RESULTS AND CONCLUSION:Patients from both groups successfuly completed fibre bronchoscopy. The oxygen saturation, mean arterial pressure and heart rate in the two groups were not significantly different (P > 0.05). However, the test group (one case of bucking) was superior to the control group (seven cases of sore throat) in terms of adverse events (P < 0.05). The simple mask can improve the oxygen supply and reduce the adverse events.

Objective To investigate the effect of health education based on transtheoretical model (TTM) on self-efficacy, health belief and health behavior of patients with coronary heart disease ( CHD). Methods A total of 122 patients with CHD were randomly divided into the observation group and the control group according to the random number table method. 61 cases in the control group were given conventional one-to-one health education, while 61 cases in the observation group were given health education based on TTM. The scores of GSES, the self-designed health belief scale for coronary heart disease and HPLP-Ⅱ were compared between the two groups before and after the invention. Results The self-efficacy and health belief of two groups were improved by the invention. The score of GSES in the observation group was (30. 5 ± 5. 9), which was significantly higher than (27. 2 ± 5. 6) in the control group after invention (t=3. 168,P<0. 01). The scores of four dimensions and total score of health belief scale were (2. 68 ± 0. 63),(2. 71 ± 0. 64),(2. 53 ± 0. 59), (2.80 ±0.51),(2. 61 ± 0. 58), which were significantly higher than (2. 40 ± 0. 58),(2. 47 ± 0. 47), (2. 25 ± 0. 62),(2. 61 ± 0. 50),(2. 41 ± 0. 52) in the control group after invention (t=2. 554,2. 361,2. 556, 2. 078,2. 005;P<0. 05). Conclusions The health education based on TTM can improve self-efficacy of health behavior change for patients with CHD, enhance health belief and is conductive to the establishment of health behavior.

Objective To suevey the family nursing knowledge′s recognition and demand status in parents of children with developmental dislocation of the hip (DDH), and provide advices for clinic, so as to give targeted nursing support for parents. Methods Using self design questionnaire, a total of 49 parents whose children had close reduction of hip joint and human position plaster fixation from August 2012 to April 2013 in Jiangyin, Jiangsu Province, were selected to investigate the recognition and demand of family nursing knowledge. Results The total cognitive score of parents to family nursing knowledge was (21. 12 ± 5. 95) and total demand score was (36. 26 ± 2. 94). Different educational background, occupation, place of residence and discharged time, parents had different cognition of family nursing knowledge (P<0. 05); low education level, physical labor, and non-urban parents had lower cognition. And non-urban parents had lower demand ( P <0. 05). Conclusions In current clinical practice, we should strengthen family nursing and knowledge propaganda, especially the families′ cognition and demand of low educational background, worker and farmer and so on manual workers, and non-urban parents, at the same time we should pay attention on the training of parents having children discharged for 0-3 months.

OBJECTIVETo determine the effects of different estrogen concentrations on the proliferation and differentiation of human embryo mandibular condylar chondrocytes (MCCs).

METHODSIn vitro-cultured MCCs were incubated and treated with estrogen at different concentrations. The MCCs were identified by toluidine blue and type II collagen immunocytochemical methods, and cell proliferation was monitored using methyl thiazolyl tetrazolium assay.

RESULTSThe addition of 10(-12) or 10(-6) mol x L(-1) exogenous estrogen inhibited the growth of MCCs, whereas addition of 10(-10) or 10(-8) mol x L(-1) exogenous estrogen promoted growth. These estrogen effects were time-dependent.

CONCLUSIONEstrogen can affect cellular MCCs proliferation and differentiation in a biphasic manner depending on the concentration. Estrogen may be vital in the maintenance of normal functions of the temporomandibular joint (TMJ) and may be involved in the progression of certain TMJ diseases.

OBJECTIVETo study the expression of the estrogen receptor (ER) and type II collagen in the mandibular condylar cartilage of the human embryo.

METHODSThe expression and localization of ERalpha, ERbeta, and type II collagen in the mandibular condylar cartilage of the human embryo were examined through hematoxylin-eosin (HE) and immunohistochemistry staining.

RESULTSType II collagen was primarily localized in the transitional and hypertrophic layers of the condylar cartilage. ERa was mostly expressed in the transitional and hypertrophic cartilaginous layers of the condylar cartilage. ERa was evenly distributed in the cell, whereas ERbeta was localized in the nuclei. No expression of type II collagen and ER was found in the fibrage and the proliferative layer although minimal expression was found in the calcified cartilage.

CONCLUSIONThe distribution of ER and type II collagen in the mandibular condylar cartilage was consistent. Estrogen can selectively combine with different subtypes of ER that regulate the ability of the condylar cartilage cells to secrete type II collagens.