Objective:To explore the application effect of a multidisciplinary collaborative model led by stomatognathic therapists, utilizing a combination of platelet-rich plasma (PRP) and moist wound dressings in the comprehensive treatment of postoperative chronic wounds, and to provide references and guidance for improving the clinical practice and management level of patients with chronic wounds.Methods:This was a prospective randomized controlled trial. Eighty-eight patients with postoperative non-healing chronic wounds who visited the Chronic Wound Care Clinic of the Third Affiliated Hospital of Guangzhou Medical University from October 2018 to February 2023 were conveniently sampled. They were randomly divided into two groups using a coin toss method: the control group ( n = 41) received standard moist wound dressing therapy, while the experimental group ( n = 47) received comprehensive treatment based on PRP combined with moist wound dressings. This treatment, led by enterostoml therpist, involved the innovative nursing technique of simultaneous injection of PRP and coagulant mixture via a three-way connector and covering with ultra-thin foam dressing. The Pressure Ulcer Scale for Healing (PUSH) score, wound area healing index (WSHI), pain level during dressing change, wound healing time, and treatment satisfaction rate were observed in both groups. Results:Among 88 cases, there were 25 females and 16 males in the control group, aged 58.00 (37.00 ± 79.00) years old. There were 31 females and 16 males in the experimental group, aged 57.00 (35.00 ± 72.00) years old. The median PUSH scores of the experimental group on the 7th, 14th, and 21th day after wound treatment were 11.00, 9.00, and 7.00, respectively, which were better than those of the control group, which were 13.00, 11.00, and 9.00. The median WSHI scores were 0.35, 0.58, and 0.84 for the experimental group, respectively, which were better than those of the control group, which were 0.09, 0.30, and 0.50. The differences between the two groups were statistically significant ( Z values ranged from 4.08 to 8.20, all P<0.01). On the 7th, 14th, and 21st day of treatment, the pain scores of the experimental group were 3.00 (2.00,3.00), 2.00 (1.00, 2.00), 0.00 (0.00,1.00) points, respectively, which were lower than the 3.00 (3.00,3.50), 2.00 (2.00,3.00), and 2.00 (1.00, 2.00) points of the control group, and the differences were statistically significant ( Z values were 2.16, 3.38, 6.14, all P<0.05). The healing time in the experimental group was (37.04 ± 25.33) days, which was shorter than that in the control group, (52.88 ± 36.58) days, and the difference was statistically significant ( t = 5.53, P<0.05). The satisfaction rate in the experimental group was 97.87% (46/47), significantly higher than 87.80% (36/41) in the control group ( χ2 = 3.13, P<0.05). Conclusions:Under the leadership of stomatognathic therapists, the multidisciplinary collaborative model, employing comprehensive treatment techniques centered around PRP combined with moist wound dressings, demonstrated significant therapeutic efficacy for postoperative chronic wounds including shortening healing time, accelerating healing speed, reducing wound pain, and improving patient satisfaction, indicating high clinical application value and social benefits.

Objective:To establish an irritant contact dermatitis(ICD)model induced by sodium dodecyl sulfate(SDS)in mice,and explore its endotype to provide an experimental and theoretical basis for subsequent precise treatment.Methods:Mice were randomly divided into two groups(model group and control group),4%SDS was topically applied to induce ICD in mice,saline was used on control group,the dose and frequency were consistent with model group,and the skin lesions of mice were observed.Epidermal thickness and inflammatory cell infiltration were analyzed by HE staining,toluidine blue staining and immunofluorescence staining.Real-time quantitative PCR was performed to investigate mRNA expression levels of cytokines.Results:Compared with control group,mice in ICD model group showed epidermal thickness on the back of neck,and the numbers of inflammatory cells were increased in dermis.The number of neutrophils,macrophages and T cells were increased.Expressions of Il17a and Il17f mRNA levels were increased.Conclusion:SDS-induced ICD model is successfully established,with the elevated infiltration of neutrophils,macrophages and T cells,and secretion of type 17 cytokines.

Pediatric idiopathic nephrotic syndrome (INS) is characterized by massive albuminuria, hypoproteinemia, edema and hyperlipidemia, with a long course and high probability of relapse and prolongation. Long-term complications caused by long-term usage of hormones and immunosuppressants in children with INS seriously affect their physical and mental health and quality of life. Most children with steroid-sensitive nephrotic syndrome can be cured before adulthood, while some of them relapse in adulthood. Long-term prognosis of children with steroid-resistant nephrotic syndrome is poor. There have been few studies in China followed the long-term outcomes and its related factors of children with INS over 10 years. The paper reviewed the literatures on the long-term outcomes of children with INS, including renal survival, growth, mental health, learning and work, marriage and fertility, disease recurrence and long-term related complications, to explore the factors related to the poor long-term outcomes of children with INS and to assist in clinical decision-making and follow-up management.

Objective:To explore the differences of the resting-state functional connectivity(FC) between goal-directed network and habituation networks in patients with early- and late-onset obsessive compulsive disorder (OCD) and the correlation between the strength of FC in the differential brain regions and cognitive flexibility.Methods:From October 2019 to April 2021, 40 patients with OCD were included in this study, including 22 patients with early-onset OCD and 18 patients with late-onset OCD.The cognitive flexibility of all subjects was assessed using the Wisconsin card sorting test (WCST), the Stroop task and the trail making test (TMT). The brain regions which were associated with goal-directed network(caudate, orbitofrontal cortex, ventromedial prefrontal cortex, and anterior cingulate cortex) and the brain regions which were associated with habituation network(putamen, supplementary motor area and insula) were selected as FC regions of interest (ROI). The DPABI and SPM12 plug-ins in the matlab2011a platform were used for whole brain FC analysis to compare the difference of FC between patients with early-onset OCD and patients with late-onset OCD on the two networks.The data were analyzed by SPSS 25.0 with χ2 test, independent samples t-test, and Pearson correlation analysis. Results:Compared with patients with early-onset OCD, patients with late-onset OCD had significantly enhanced FC of the left supplementary motor area with the left putamen and left insula.The total number of persistent errors of WCST in patients with late-onset OCD was greater than that in patients with early-onset OCD ((20.61±11.30), (14.95±8.94), P<0.05). The FC of the left putamen-left supplementary motor area was significantly and positively correlated with the total number of sustained responses ( r=0.678, P=0.003) and the total number of incorrect responses ( r=0.590, P=0.013) in patients with late-onset OCD.The FC of the left supplementary motor area-left insula was significantly positively correlated with the number of responses required to complete the first classification in patients with late-onset OCD ( r=0.485, P=0.049). Conclusion:Patients with late-onset OCD have stronger habituation network FC than patients with early-onset OCD, and the enhanced FC correlates with patients' cognitive flexibility performance, while late-onset OCD has more impaired cognitive flexibility than early-onset OCD.

Objective: To understand the situation of school health staff and the allocation of health equipment in primary and secondary schools in Jinan. Methods: A questionnaire survey was conducted in CDC and public primary and secondary schools to describe the situation of the school health staff and health equipment in primary and secondary schools. Results: There were 23 school health staffs in CDC, among which 26.1% were full time. The total staffing ratio of school doctors was 13.9%, with the compliance rate of 19.8%. The total allocation rate of health care teachers was 74.7%, 92.2% of them were part time. The total staffing ratio of school doctors in boarding schools was 20.0%, with a staffing compliance rate of 12.5%. The total allocation rate of school doctors and health care teachers in boarding schools was 66.7%. 16.8% of public primary and secondary schools and 23.3% of public boarding primary and secondary schools did not have any health staffs. 27.6% of public primary and secondary schools are equipped with infirmary, 70.8% with health rooms, and 10.3% without any. The configuration of necessary equipment in infirmary and health care rooms were less than 100%. Conclusion There is a serious shortage of school health staffs and health equipment in primary and secondary schools in Jinan. Attention and investment in school health should be strengthened, and resources should be rationally allocated to ensure the health of primary and secondary school students.

Objective:The association between leptin receptor (LEPR) Q223R (Gln>Arg) gene polymorphism and systemic lupus erythematosus (SLE) remains controversial.In this study, a meta-analysis was used to comprehensively evaluate the association between LEPR Q223R gene polymorphism and SLE susceptibility.Methods:Case control studies on the relationship between LEPR Q223R gene polymorphism and SLE susceptibility were comprehensively searched by Medline (PubMed), Web of Science, CNKI, Wanfang digital journal full-text database, etc., and the search time was up to April 2020.The data of A/G allele frequency and AA/AG/GG genotype in SLE patients and healthy controls were extracted, the odds ratio ( OR) value and 95% confidence interval ( CI) were used as the combined effect-size indicators to analyze the correlation between allele, genotype and SLE risk.The heterogeneity among studies was analyzed quantitatively, and the publication bias was evaluated by Begg and Egger’s test. Results:A total of 7 case-control studies from 4 studies were retrieved.A total of 9 052 patients with SLE and 8 146 healthy controls were included in the meta-analysis.The results showed that there was no significant association between LEPR Q223R A/G gene polymorphism and SLE susceptibility, and the OR of A allele in LEPR Q223R gene locus associated with SLE risk was 1.03(95% CI: 0.92-1.14). The dominant (AA+ AG vs GG) and recessive (AA vs AG+ GG) models both suggested that LEPR Q223R A/G gene polymorphism was not associated with SLE, and the combined OR (95% CI) was 0.88(0.15-5.37) and 1.13(0.37-3.49), respectively.The results also showed that the distribution of LEPR Q223R genotype was different among different populations, and the inter-study heterogeneity was large. Conclusion:The existing evidence is insufficient to indicate that there is an association between LEPR Q223R A/G gene polymorphism and SLE susceptibility, which needs to be confirmed by further studies.

Objective: To investigate the knowledge, attitude and practice(KAP) of salt and health among primary school students in Jinan, and to provide scientific evidence for targeted interventions. Methods: 1 293 primary school students of grade 4 in Jinan were randomly selected by cluster sampling method, and a self-designed questionnire on "salt and health" was applied among them. Results: Among grade 4 students in Jinan, 35.73% were aware the diagnostic criteria of hypertension in adults, 67.67% were aware that eating less salt help lowering blood pressure. The proportion of students who knew the recommended daily intake of salt for adults, children aged 1-6 years and for children aged 7-13 years was 55.68%, 53.21% and 50.62%, respectively. The proportion of students who favor lower intake of salt(70.84%), actively choose low-salt food(65.89%), received publicity or education on low-salt diet(72.85%), pay attention to the salt/sodium content when purchasing packaged food(73.01%), believe that packaged food should be labelled with salt/sodium content(84.45%), and heard of low-sodium salt (alternative salt)(57.77%).The boys and girls were found no statistical significance in the differences of their attitude toward controlling salt, skills and behaviors(P<0.05),with 68.05% of boys and 73.91% of girls agreed, 13.76% of boys and 9.24% of girls reported no attention paid. There was no significant difference in other salt control attitudes, skills and behaviors between boys and girls(P>0.05). Conclusion A series of interventions taken by Shandong Ministry of Health Action on Salt Reduction and Hypertension (SMASH) within schools show positive effects. However, gaps in salt taste and salt intake among primary school students still exist, suggesting health education should be further strengthened in respect of daily salt intake recommendation, salt intake attitude and behaviors, especially for boys.

Aim To study the apoptosis effect of cucurbitacin Ⅱa on non-small cell lung cancer cell lines NCI-H460 and A549 and its underlying mechanism.Methods Cell viability was assessed by CCK-8 assay.The apoptosis effect and cell cycle arrest were detected by Flow cytometry.Western blot was employed to detect the related protein.Results The proliferation of lung cancer cell lines NCI-H460 and A549 was inhibited by CuⅡa, which showed cytotoxic activity with IC50 values of 224.9 nmol·L-1 and 108.3 nmol·L-1 against NCI-H460 and A549 respectively.CuⅡa induced the cells apoptosis and cell cycle arrest at G2/M phase.The results of Western blot showed CuⅡa inhibited the phosphorylation of STAT3 and Cofilin in a dose-dependent manner.Further, CuⅡa inhibited the phosphorylation of Aurora A, in line with the important characteristics of anti-tumor effect of Aurora A kinase inhibitor with blocking cells in the G2/M phase.Conclusion CuⅡa has obvious anti-tumor effect against non-small cell lung cancer, which suggests its value as a lead compound for lung cell carcinoma.

ABSTARCT:Objective To investigate the effects of the transcription factor SRY-related high-mobility-group box 2 (Sox2)related to stem cells on the invasion and migration of cervical squamous carcinoma cell line SiHa and its mechanism.Methods The expression of Sox2 was detected in Sox2 stably over-expressed cell line SiHa-Sox2 and negative control SiHa-EGFP cells by RT-PCR and Western blotting,respectively.The effects of Sox2 on the invasion and migration capacities of SiHa cells were detected by wound-healing assay and transwell assay.The expression of β-catenin was detected by Western blot.Results Compared with that of SiHa-EGFP cells,the expression of Sox2 at both mRNA and protein levels was obviously upregulated in SiHa-Sox2 cells.The migration and invasion capacities of SiHa-Sox2 cells were increased significantly (P <0.01 ),and the expression of β-catenin increased dramatically compared with that of the control cells.Conclusion Sox2 promotes the invasion and migration capacities of SiHa cells by increasing the expression of β-catenin and activating Wnt signaling pathway, which contributes to the development of cervical cancer.

Objective To explore the association between the intakes of cured meat and the risk of colorectal cancer,and analyze the role of the interaction between cured meat intake and dietary habit or lifestyle for the risk of colorectal cancer.Methods A well-designed questionnaire were used to collect retrospective information about demography,lifestyle and dietary habit from 400 patients diagnosed with colorectal cancer and 400 controls matched by age and gender.Logistic regression analysis was conducted to examine the possible association between the intake of cured meat and the risk of colorectal cancer.Generalized multifactor dimensionality reduction (GMDR) was used to analyze the interaction between cured meat intake and other dietary habits or lifestyle factors.Results Data from conditional logistic regression analysis showed that compared with the people who had ＜8 times per year of cured meat intake,those had 8-23 times of cured meat intakes (OR=3.480,95％CI:2.022-5.991,P＜0.001),24-47 times of cured meat intakes (OR=4.526,95％CI:2.553-8.025,P＜0.001),≥48 times of cured meat intakes (OR=5.472,95％CI:3.105-9.642,P＜0.001) had increased risk of colorectal cancer.There was a multiplied interaction between cured meat intake and pepper intake (OR=0.379,95％CI:0.190-0.758,P=0.009).The synergistic effect existed between cured meat intake and pickled vegetable intake;and the antagonism existed between cured meat intake and tea-drinking habit.GMDR analysis showed that interaction among red meat intake,pickled vegetable and cured meat intake on the risk of colorectal cancer might exist (P=0.001).Conclusions With the increase of frequency of cured meat intake,the risk of colorectal cancer increased gradually.The synergistic effect might existed between pickled vegetable intake and cured meat intake.Tea-drinking,pepper intake and cured meat intake might be antagonism in the pathogenesis of colorectal cancer.Red meat intake,pickled vegetable intake and cured meat intake might have combined effect to increase the risk of colorectal cancer.