Objective : To investigate whether NaHS，a hydrogen sulfide donor，can improve myocardial injury in sepsis by inhibiting oxidative stress and activating the Xc －/ GPX4 signaling pathway in ferroptosis． Methods : Lipopolysacc-haride(LPS) induced H9c2 in rat cardiomyocytes to form an in vitro model of myocardial injury in sep- sis，which was divided into Control group，LPS group and LPS + NaHS group．The kits were applied to detect the changes of cardiomyocyte viability，Fe2 + ，LDH and CK-MB，determine the levels of oxidative stress indexes GSH and MDA，detect the changes of cellular ROS and mitochondrial membrane potential levels by fluorescent probes， and detect the expression levels of ferroptosis regulatory proteins SLC7A11 and GPX4 by Western blot. Results: Compared with the Control group，H9c2 cell viability decreased，Fe2 + concentration increased ，GSH ，MDA and ROS levels increased，mitochondrial JC-1 monomer increased ，expression levels of ferroptosis regulatory proteins SLC7A11 and GPX4 decreased，and cell damage increased after LPS stimulation (P＜0. 05) ．Compared with the LPS group，NaHS attenuated LPS-induced H9c2 cell injury and elevated Fe2 + concentration，decreased the level of LPS-induced oxidative stress in H9c2 cells ，and increased the expression levels of ferroptosis regulatory proteins SLC7A11 and GPX4 (P＜0. 05 ) ． Conclusion The mechanism by which NaHS attenuates myocardial injury in sepsis may be related to the inhibition of oxidative stress and activation of the Xc －/ GPX4 signaling pathway in fer- roptosis.

Objective:To measure and analyze the spherical radius of Monson of normal young people in Guangdong province using cone-beam CT (CBCT), and to establish a personalized measurement method of the spherical radius of Monson to provide a reference for clinical application of Monson spherical radius in occlusal reconstruction.Methods:Sixty healthy young adults from physical examination population at Stomatology Hospital of Guangzhou Medical University [30 males and 30 females, aged (22.1±2.0) years 18-26 years) were recruited, and their CBCT were taken. Three-dimensional reconstruction of CBCT data was carried out, and the reconstructed models were fixed, traced and measured. The difference of Monson spherical radius between male and female was compared by using a single sample t-test. Results:The Monson spherical radius was (100.72±4.89) mm. The Monson spherical radius of male and female were (103.48±4.19) mm and (97.97±3.93) mm respectively. The difference between male and female was statistically significant ( P<0.01). Conclusions:CBCT can be used to accurately measure the spherical radius of Monson and can be used as a reference for reconstruction of occlusal plane.

Objective To study the effect of hyperbranched polyester(HBP) on mechanical properties of the conventional resin matrix.Methods Two hyperbranched monomers(HBP2-X and HBP2,-Y)were synthesized and incorporated at 40％(by mnass) into a 2:1(by mass) bisphenol-A diglycidyl methacrylate (Bis-GMA):triethylene glycol dimethacrylate(TEGDMA) resin.Bis-GMA/TEGDMA without the incorporation of hyperbranched polyester was used as control(n=8 per group).The mechanical properties of the modified neat resin,including polymerization volumetric shrinkage,Vickers hardness,water absorption and dissolution rate,as well as the flexural strength,compressive strength and diametral tensile strength of the composite resin with different content of filler(0％,30％,50％,70％) were measured.Mechanical properties of the composite resin were measured by universal testing machine.The results were analyzed by the ANOVA and LSD-t-test.Results The resin with HBP greatly decreased the polymerization shrinkage of the composite resin,and the value of group X was (6.32±0.49)％,and that of group Y was (6.31±0.68)％,whereas that of the control group was (8.14± 0.53)％.The value of volumetric shrinkage of the modified groups were significantly lower than that of the control group(P＜0.05).By adding HBP,the hardness value of group X([198± 5] MPa) and group Y([177± 4] MPa) were significantly lower than that of the control group ([214±6] MPa)(P＜0.05).The HBP significantly influenced the water sorption and solubility of the neat resin (P＜0.05).For the composite materials,with 70％ inorganic filler,there were no significant difference in the value of flexural strength among the groups(P＞0.05).There was no significant difference in the value of compressive strength,between group X([244± 13] MPa) and the standard group([234± 17] MPa)(P＞0.05).However,they were significantly higher than that in group Y([204 ± 24] MPa)(P＜0.05).For the value of diametral tensile strength,there was no significant difference between group Y([36.4±2.1] MPa) and the control group([34.4±2.9] MPa)(P＞0.05),but both were significantly lower than that in group X([41.1±3.0] MPa)(P＜0.05).Conclusions Although there were certain effect on the mechanical properties of the resin that was modified with the HBP,we should make further analysis and study based on the property of low volumetric shrinkage,to strive for the development of low volumetric shrinkage of HBP,without affecting its mechanical properties.

OBJECTIVE:To systematically review the efficacy of folic acid in the prevention of gastrointestinal(GI)tumors in elder,and provide evidence-based reference for the clinical treatment. METHODS:Retrieved from Cochrane Library,PubMed,EM-Base,Web of Science,CMB,CJFD,VIP and Wanfang Database,the randomized controlled trials (RCT) about folic acid (test group)versus placebo(control group)in the prevention of GI tumors in elder(age>50 years old). After quality evaluation and da-ta extract,Meta-analysis was performed buy using Rev Man 5.3 statistics software. RESULTS:A total of 9 RCT were included,in-volving 29 494 patients. Results of Meta-analysis showed there was no significant difference in the incidence of GI tumors between 2 groups[RR=0.91,95%CI(0.74,1.12),P=0.38]. Results of subgroup analysis showed the incidence of GI tumors was not affected by GI tumor stage,age,basic serum folic acid level,type and dose of folic acid and follow-up time during follow-up period. CON-CLUSIONS:Folic acid supplement can not effectively prevent the incidence of GI tumors in elder. The incidence of GI tumors in elder with different ages and baseline of folic acid are not decreased by any dose and duration,without obvious adverse reactions. It indicates that folic acid supplement should be careful and give full conscideration to the patients’general situation. Due to the limit of methodological quality and sample size,it remains to be further verified with more rigorously designed and long-term follow-up of large-scale RCT.

Objective To establish the HPLC fingerprint for the quality control of Yanhuanglian Injections.Methods HPLC Chromatography method was used.The conditions included a Shim-pack CLC-ODS column(250 mm?6.0 mm,5 ?m),the gredient elution was adopted with acetonitrile-buffer solution(1∶1),the detection wavelength was at 285 nm,and the flow rate was 1.0 mL/min.The Operating Standard of Similarty Evaluation System for Chromatographic Fingerprint of Chinese Materia Medica(Version 2004A)was used to calculate.Results Similarity of 13 batches of injections was over 0.95,the fingerprint of Yanhuanglian Injections was established,and 12 common peaks were indicated.Conclusion This method can be applied to the quality control of Yanhuanglian Injections.