Objective:To study the risk factors of bronchopulmonary dysplasia (BPD) in extremely preterm/extremely low birth weight infants(EPT/ELBWIs).Methods:From June 2019 to March 2022, clinical data of EPT/ELBWIs with gestational age <28 weeks or birth weight <1 000 g admitted to NICU of our hospital were retrospectively analyzed. They were assigned into BPD group and non-BPD group. Multivariate logistic regression analysis was used to find the independent risk factors for BPD and receiver operating characteristic (ROC) curve was used to determine the cut-off value for BPD. The incidences of BPD of the two groups were compared and the correlation between independent risk factors and BPD severity was analyzed.Results:A total of 82 EPT/ELBWIs were enrolled, including 47 (57.3%) in BPD group and 35 (42.7%) in non-BPD group. The BPD group had longer duration of both invasive and non-invasive mechanical ventilation (MV) [24.0(8.0, 38.0)d vs. 6.0 (0.2, 11.6)d, (38.4±14.5)d vs. (32.4±10.9)d], lower birth weight [906 (800, 970)g vs. 980 (880, 1 050)g],higher incidences of ureaplasma urealyticum colonization (48.9% vs. 22.9%) and hemodynamically significant patent ductus arteriosus (hsPDA) (76.6% vs. 51.4%) than the non-BPD group(all P<0.05). Multivariate logistic regression analysis showed that the independent risk factor for BPD was the duration of invasive MV ( OR=1.003, 95% CI 1.001-1.005). The cut-off value of invasive MV duration for predicting BPD was 14.4 d. The duration of invasive MV was positively correlated with BPD severity ( r=0.604, P<0.001). Conclusions:BPD is more likely to occur in EPT/ELBWIs with longer duration of invasive MV.

Objective To explore the relationship between cerebral hemorrhage and the occurrence of depression after craniocerebral injury surgery.Methods From March 2015 to March 2017,70 patients with craniocerebral injury in our hospital were selected as the subjects of study.They were divided into the observation group (n =30) and the control group (n =40) according to whether there was cerebral hemorrhage after operation.The National Institutes of Health Stroke Scale score (NIHSS),Hamilton Depression Scale score (HAMD),Glasgow Coma Score (GCS) and the levels of interleukin (IL)-1,IL-6 and high sensitivity C-reactive protein (hs-CRP) in patients with different degrees of cerebral hemorrhage were compared.The correlation between HAMD score and other indexes was analyzed.The influencing factors and independent risk factors of depression were analyzed.Results The levels of NIHSS,HAMD score and serum IL-1,IL-6 and hs-CRP in the observation group were higher than those in the control group after operation (t =13.655,36.587,45.274,46.334,70.489,P ＜ 0.05),while the GCS score was lower than that in the control group (t =15.517,P ＜ 0.05).Compared with mild and moderate cerebral hemorrhage,the NIHSS,HAMD scores and serum IL-1,IL-6 and hs-CRP were higher in patients with severe cerebral hemorrhage (F =8.825,10.254,23.615,18.745,7.245,P ＜ 0.05),while the GCS score was lower (F =7.459,P ＜ 0.05).The correlation analysis showed that the postoperative HAMD score was positively correlated with NIHSS score and serum IL-1,IL-6 and hs-CRP (r =0.289,0.364,0.333,0.258,P ＜ 0.05)and negatively correlated with the GCS score (r =-0.276,P ＜ 0.05).Univariate analysis showed that the level of culture,personality,cerebral hemorrhage degree and serum IL-6 and hs-CRP were the influencing factors of postoperative depression in patients with craniocerebral injury (t orx2 =6.076,6.300,8.361,30.224,99.575,P ＜ 0.05).Multivariate analysis showed that cerebral hemorrhage and hs-CRP were an independent risk factor for postoperative depression (P ＜ 0.05).Conclusions Cerebral hemorrhage after craniocerebral injury is closely related to the occurrence of depression,which may be related to the increase of stress-induced inflammatory factors after cerebral hemorrhage.

Objective To observe the effect of scalp-body acupuncture plus psychological intervention in treating post-stroke depression.Method Seventy-eight patients with post-stroke depression were divided into a treatment group (40 cases) and a control group (38 cases). In addition to ordinary neurological treatments, the treatment group also received scalp-body acupuncture plus psychological intervention while the control group also received Fluoxetine hydrochloride (Prozac). The interventions were given once a day, 4 weeks as a treatment course, for 2 courses in total. Hamilton Rating Scale for Depression (HAMD) and neurological impairment severity scale (NISS) were observed before intervention, after 1 treatment course and at the end of the intervention.Result After intervention, the HAMD and NISS scores dropped significantly in both groups, and the treatment group was markedly better than the control group (P＜0.05). The total effective rates of anti-depression and recovery of neurological function in the treatment group were significantly higher than those in the control group (P＜0.05).Conclusion Scalp-body acupuncture plus psychological intervention can produce a content efficacy in improving depression and recovering neurological function.

BACKGROUND:With increased age,bone marrow mesenchymal stem cells(BMSCs)are influenced with regard to quantity and quality,which will induce great damages to the donors.Many studies have focused on seeking substitute MSC source.In contrast it remains controversial whether umbilical cord blood contains MSCs.OBJECTIVE:To isolate MSCs from human umbilical cord blood,and to detect their biological properties.METHODS:Umbilical cord blood samples were sterilely isolated using Percoll density gradient centrifugation to harvest intermediate layer cells.DMEM medium containing fetal bovine serum,penicillin,streptomycin and L-glutamine was added.Following several adherences and purification,the floating cells were discarded.Thus,many adherent cells with a confluence were collected.When cells were 60% 80% confluent,cells were digested by trypsin for subculture.Cells at passages,1,5 and 9 were obtained and their morphological changes were observed.Cell surface antigens were measured using flow cytometry.Growth curves were drawn,and cell viability was determined utilizing MTT.RESULTS AND CONCLUSION:Isolated umbilical cord blood MSCs presented an even size,showing spindle or star-shape fibroblasts-like cells.Umbilical cord blood MSCs at 1,5,9 passages were greatly positive for CD29,CD105 and CD166,but weakly positive for CD34 and CD45.Following 5 days of incubation,cells entered logarithmic growth phase.The number was decreased at day 9.Population doubling time was(53.5±8.32)hours.Cells grew well.Cells at 1-7 passages showed similar viability(P ＞ 0.05).Till passage 9,cell proliferation viability was decreased,but no significant difference was determined(P ＞0.05).Results verified that MSCs can be successfully isolated from umbilical cord blood in vitro.Cells at passages 1-9 presented a good reproductive activity.

A dynamics model of human lower extremity, which combines musculotendon dynamics and muscle excitation-contraction dynamics, is presented. With this model, a motion process of normal gait during swing phase is numerically analyzed by use of the optimal control theory. The model was verified using experimental kinematics, muscles activation, and electromyographic data. The result showed that the tri-phasic activation pattern and synergistic muscles displayed during a normal gait in swing phase. The pattern consists of three distinct phases, i. e., acceleration during moving initiation, braking the moving segment, and posture control at the final specified position.
Biomechanical Phenomena ; Computer Simulation ; Electromyography ; Gait ; physiology ; Humans ; Kinetics ; Lower Extremity ; physiology ; Models, Biological ; Muscle, Skeletal ; physiology ; Tendons ; physiology

Ligaments are the main parts which stabilize the knee joint. How to analog the ligaments in biomechanical model will affect the characteristics of the human knee dynamics and in the computation of the stress in ligaments between two bones. This symposium is aimed at the survey of the simplified method of the ligaments via mechanical parameters, and providing an exact method of constructing model.
Biomechanical Phenomena ; Humans ; Knee Joint ; anatomy & histology ; physiology ; Ligaments, Articular ; anatomy & histology ; physiology ; Models, Anatomic ; Models, Biological

Evaluation of human gait function is of great significance in clinical medicine and rehabilitation engineering. A quantitative gait evaluation method using principal component analysis was proposed. The evaluation steps included that a series of characteristic index was performed by the gait parameters with a gait detection, and the index was normalized, quantified and summarized by principal component analysis. Then the evaluation results were shown in formulation, figures and tables. The examples showed that this system could evaluate the recovery of the gait by treatment.
Evaluation Studies as Topic ; Gait ; physiology ; Humans ; Principal Component Analysis

In order to discuss the evaluation method of human upper limb movements, the patterns of movement coordination during healthy people prehension have been researched. Eight subjects were asked to perform different reaching-grasping and drinking water from the cup tasks with different indices of difficulty, and the arm movement trajectories and the main muscles group electromyography (EMG) data were collected. To explore the prehension control mechanism, a comparison has been made between the solution of the theoretic calculation and the experimental data. The results show that the topological invariance was observed in the trajectories of different task performance, and the linear relationships between joints covariation were exhibited. Moreover, the different muscles were controlled and combined into units of synergistic muscular group necessary to reach and grasp the goal.
Adult ; Electromyography ; Hand ; physiology ; Hand Strength ; physiology ; Humans ; Male ; Models, Biological ; Range of Motion, Articular ; physiology ; Upper Extremity ; physiology

OBJECTIVETo determine the effects of raloxifene hydrochloride (RLX) on bone mineral density (BMD), bone metabolism markers and serum lipids in healthy postmenopausal women in Beijing.

METHODSA multicenter, randomized, double-blind, placebo-controlled study was conducted in a total of 204 healthy postmenopausal women (age 59.5 +/- 5.0 years and weight 62.8 +/- 8.7 kg) treated with either RLX 60 mg (n = 102) or placebo (n = 102) daily for 12 months. BMD, serum lipids, and bone markers were measured before and after drug administration.

RESULTSCompared with placebo, RLX produced a significant increase in both total lumbar spine and total hip BMD. For the lumbar spine, percentage increase in total BMD was 2.3% with RLX compared with a decrease of 0.1% with placebo (P < 0.001). Corresponding values for total hip BMD were a 2.5% increase for RLX and a 1.1% increase for placebo (P = 0.011). For biochemical markers of bone metabolism, serum osteocalcin and C-telopeptide, percentage decreases were 27.65% and 24.02% in RLX-treated subjects. Corresponding values in placebo were a 10.64% decrease and a 15.75% increase (RLX compared with placebo, both P < 0.001). For total cholesterol and low-density lipoprotein cholesterol levels, percentage decreases were 6.44% and 34.58% in the RLX-treated group. Corresponding values in placebo-treated patients were a 1.44% increase and a 19.07% decrease (RLX compared with placebo, both P < 0.001). No differences were found for high-density lipoprotein cholesterol or triglyceride levels between the two groups. Only 5 subjects discontinued early owing to an adverse event (3 in the RLX group and 2 in the placebo group).

CONCLUSIONSThis study confirms that RLX exerts positive effects on the skeleton, increasing BMD and decreasing biochemical markers of bone metabolism, and has a positive effect on the overall serum lipid profile in postmenopausal women in China.

Aged ; Biomarkers ; blood ; Bone Density ; drug effects ; Bone and Bones ; metabolism ; China ; Estrogen Antagonists ; pharmacology ; Female ; Humans ; Lipids ; blood ; Middle Aged ; Postmenopause ; physiology ; Raloxifene Hydrochloride ; pharmacology ; Selective Estrogen Receptor Modulators ; pharmacology