Objective To study the predicting value of plasma BNP and serum Cys C on heart failure caused by arrhythmogenic right ventricular cardiomyopathy(AVRC).Methods Thirty-two patients with AVRC were chosen.The plasma BNP and serum Cys C were tested.All subjects were followed up for 18 months to observe the happening of heart failure.Results The levels of plasma BNP and serum Cys C at admission in ARVC patients showed the increasing trend along with the decrease of heart function,and the difference among the groups with different heart functions were statistically significant(P＜0.05).The multivariate Logistic regression analysis showed that plasma BNP(OR=4.118) and serum Cys C (OR=6.358)were the independent predicting factors for heart failure in AVRC patients.When BNP and Cys C had the cutoff values of 732.45 ng/L and 2.16 mg/L,their sensitivity and specificity for predicting heart failure were highest.In the survival analysis with the heart failure occurrefice at follow up as the endpoint outcome,the survival rate of the low risk BNP group (≤732.45 ng/L)was higher than that of the high risk BNP group(P＜0.01),and the survival rate of the low risk Cys c group (≤2.16 mg/L)was higher than that of the high risk Cys C group(P＜0.01).Conclusion The plasma BNP and serum Cys C levels had highere predicting value on heart failure caused by AVRC.

To investigate the related substances of tenofovir, 3 related substances were synthesized. RP-HPLC analytical method was developed using Agilent ZORBAX SB-C18 column. The mobile phase composed of methanol and 0. 02 mol/L potassium dihydrogen phosphate. Linear gradient elution was used and the detection wavelength was 260 nm. 3 impurities were found in tenofovir. The established method was accurate and reproducible, and it can be applied for the related substances test in tenofovir.