[Objective]To explore the medication rule of TCM compound patent in the treatment of gout,and analyze the mechanism of action of the core herbs based on the national patent database.[Methods]The Chinese herbal compound patent database for gout treatment was retrieved and entered into the Website of China Patent Announcement of The State Intellectual Property Office.The characteristics of medications were analyzed by frequency statistics,Apriori algorithm,factor analysis and systematic clustering method.The Chinese herbs targets of the core herbs combinations were obtained through Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform(TCMSP),and the gout disease targets were obtained from Human Gene database(GeneCards)and Online Mendelian Inheritance in Man(OMIM)database.The intersection of the two targets was imported into Cytoscape 3.9.1 software,and the Cluego plug-in was used to complete the Kyoto Encyclopedia of Genes and Genomes(KEGG)pathway enrichment analysis of the herb combinations.The intersection targets were imported into STRING database to obtain protein-protein interaction(PPI)network,and some key targets were selected for molecular docking with corresponding active compounds of Chinese herbs.[Results]A total of 508 Chinese herbal compound patents were included in the treatment of gout,involving 401 Chinese herbs,and the frequency of herbs was 6 082 times.Chinese herbs for diuresis-inducing and dampness-draining were mainly used,followed by herbs for tonifying deficiency,clearing heat,promoting blood circulation and removing blood stasis.The analysis of association rules obtained 3 binomial association rules and 5 trinomial association rules.Eight common factors were extracted by factor analysis.Three groups of herbs combinations were obtained by systematic clustering analysis,and 56 common biological pathways of herbs combination enrichment were found.Molecular docking results showed that the combination of Quercetin and tumor necrosis factor(TNF)was the best,followed by Baicalein and interleukin-6(IL-6),and the combination of Quercetin and adenosine triphosphate binding cassette super family G member 2(ABCG2)was good.[Conclusion]The national patent prescriptions for gout treatment based on the core pathogenesis of"turbid dampness and blood stasis"are mainly from three aspects of dampness,blood stasis and deficiency.The core herbs combinations are often supplemented or subtracted based on Simiao Powder,Fuling Zexie Decoction and Siwu Decoction.Chinese herbs mainly exert their effects through multiple signaling pathways such as TNF,hypoxia inducible factor-1(HIF-1),Toll-like receptors(TLRs)and IL-17 and promote excretion of uric acid.

Objective:To investigate the status quo and influencing factors of the somatization of depressive emotions among college students, in order to provide a theoretical basis for the reform of mental health education and the formulation of operable mental health counseling for college students.Methods:Totally 297 college students from two institutions of higher education in Heilongjiang Province were selected by convenience sampling and investigated with the College Student Depressive Emotion Somatization Questionnaire, Attitudes Toward Seeking Professional Psychological Help Scale-Short Form (ATSPPH-SF), and the Type D Personality Scale (DS14) .Results:The total score for the College Student Depressive Emotion Somatization Questionnaire among the 297 students was (28.49±8.07), and the total score for the ATSPPH-SF was (21.98±2.73). Multiple linear regression analysis showed that the influencing factors for the somatization of depressive emotions in college students were professional psychological help-seeking attitudes and whether they had a Type D personality ( P < 0.05) . Conclusions:During university education, there should be a focus on students' personality traits and the positivity of their attitudes toward seeking professional psychological help in order to mitigate the occurrence of adverse psychological issues such as symptoms of depressive somatization.

METTL3 and METTL14 are two components that form the core heterodimer of the main RNA m6A methyltransferase complex (MTC) that installs m6A. Surprisingly, depletion of METTL3 or METTL14 displayed distinct effects on stemness maintenance of mouse embryonic stem cell (mESC). While comparable global hypo-methylation in RNA m6A was observed in Mettl3 or Mettl14 knockout mESCs, respectively. Mettl14 knockout led to a globally decreased nascent RNA synthesis, whereas Mettl3 depletion resulted in transcription upregulation, suggesting that METTL14 might possess an m6A-independent role in gene regulation. We found that METTL14 colocalizes with the repressive H3K27me3 modification. Mechanistically, METTL14, but not METTL3, binds H3K27me3 and recruits KDM6B to induce H3K27me3 demethylation independent of METTL3. Depletion of METTL14 thus led to a global increase in H3K27me3 level along with a global gene suppression. The effects of METTL14 on regulation of H3K27me3 is essential for the transition from self-renewal to differentiation of mESCs. This work reveals a regulatory mechanism on heterochromatin by METTL14 in a manner distinct from METTL3 and independently of m6A, and critically impacts transcriptional regulation, stemness maintenance, and differentiation of mESCs.
Animals ; Mice ; Methylation ; Chromatin ; Histones/metabolism* ; RNA, Messenger/genetics* ; Methyltransferases/metabolism* ; RNA/metabolism*

Objective:To explore the mediating effect of negative cognitive processing bias between type D personality and depression after percutaneous coronary intervention (PCI) .Methods:Using the convenient sampling method, a total of 340 patients who underwent PCI for the first time in Department of Cardiology in the Second Affiliated Hospital of Harbin Medical University from September to December 2021 were selected as the research objects. The General Demographic Data Questionnaire, Type D Personality Scale (DS14), Negative Cognitive Processing Bias Questionnaire (NCPBQ) and Beck Depression Inventory-Ⅱ (BDI-Ⅱ) were used to investigate. Spearman correlation analysis was used to determine the correlation between type D personality, negative cognitive processing bias and depression. The mediating effect model was established by AMOS 24.0 software to analyze the mediating effect of negative cognitive processing bias between type D personality and depression.Results:A total of 340 questionnaires were sent out in this study, and 317 valid questionnaires were recovered, with an effective questionnaire recovery rate of 93.2%. The total score of Type D Personality Scale of 317 patients after PCI was (18.05±4.15), in which the score of negative affectivity dimension was (9.51±2.33) and the score of social inhibition dimension was (8.54±2.61). The proportion of patients with type D personality is 24.9% (79/317). The score of Beck Depression Inventory-Ⅱ (BDI-Ⅱ) was (9.52±4.83), and the incidence of depression was 20.2% (64/317). Correlation analysis showed that D-type personality of patients, negative cognitive processing bias and depression were positively correlated ( P<0.05). The mediating effect analysis showed that type D personality could directly affect the occurrence of depression (effect size 0.431, P<0.001), or indirectly through negative cognitive processing bias (effect size 0.145, P<0.001) . Conclusions:The negative cognitive processing bias is an important factor in the occurrence of depression in patients with CHD and with type D personality. Improving the negative cognitive processing bias will help reduce the occurrence of depression and improve the prognosis.

Objective:To construct a home-based cardiac rehabilitation intervention system for patients after percutaneous coronary intervention, and to provide reference for improving the self-management ability and family support of home-based cardiac rehabilitation of patients after PCI.Methods:Based on the literature study and group discussions, a draft of home-based cardiac rehabilitation intervention system for patients after PCI based on empowerment theory was constructed. From January to April 2021, the Delphi method was used to conduct 2 rounds of expert consultations among 18 experts from 9 hospitals, and the items were modified according to the experts′ advice.Results:The expert positive coefficients of the 2 rounds were 94.44% and 100.00%, the expert authority coefficients was 0.91, and the Kendall coefficients were 0.188 and 0.255. Finally, a home-based cardiac rehabilitation intervention system for patients after PCI was formed, including 5 first-level items, 19 second-level items and 21 third-level items.Conclusion:The home-based cardiac rehabilitation intervention system for patients after PCI is reliable, scientificity and practical, and has guiding significance for promoting the development of home-based cardiac rehabilitation for PCI patients.

Objective:To clarify the mechanism of type D personality on plaque vulnerability in patients with coronary heart disease based on the stress-mediator model.Methods:Using the convenient sampling method, a total of 386 patients with coronary heart disease who were admitted to Department of Cardiology of the Second Affiliated Hospital of Harbin Medical University from December 2020 to May 2021 were selected as the research objects. The patients were investigated using Type D Personality Scale-14 (DS-14) , Self-rating Anxiety Scale, Self-rating Depression Scale, The Stress Appraisal Measure (SAM) and Trait Coping Style Questionnaire (TCSQ) . At the same time, optical coherence tomography was used to evaluate the plaque characteristics of patients with coronary lesions. A structural equation model was used to construct a model of influencing factors of plaque vulnerability in patients with type D personality coronary heart disease.Results:Among the patients with coronary heart disease in this study, there were 138 patients with type D personality and 200 patients in the vulnerable plaque group. The D-type personality score, anxiety, depression, negative coping and cognitive evaluation scores in the vulnerable plaque group were higher than those in the non-vulnerable plaque group, and the differences were statistically significant ( P<0.01) . The active coping score of the vulnerable plaque group was lower than that of the non-vulnerable plaque group, and the difference was statistically significant ( P<0.01) . The results of structural equation modeling showed that type D personality could directly or indirectly affect plaque vulnerability through cognitive assessment (β=0.588, P<0.01) and negative coping (β=0.189, P<0.05) . Among them, cognitive assessment (β=-0.061, 0.121; P<0.01) and negative coping (β=-0.124, 0.031; P<0.01) had indirect effects on plaque vulnerability. Negative coping (β=0.215, P<0.01) , psychological responses (β=0.241, -0.150, P<0.01) and physiological responses (β=-0.321, P<0.01) had direct effects on plaque vulnerability. Conclusions:Cognitive evaluation, coping style, psychological response and physiological response of patients with type D coronary heart disease can affect plaque vulnerability through various pathways. Therefore, strengthening the clinical screening of type D personality can early identify the high-risk population of plaque vulnerability, and at the same time, individualized and systematic psychological behavior intervention should be carried out for such population, so as to move the prevention and treatment of coronary heart disease forward.

Objective:To explore the characteristics of attention bias in patients with type D personality coronary heart disease (CHD), and provide a new theoretical perspective for elucidating the effect of type D personality on the pathogenesis of CHD and formulating prevention strategies.Methods:From March 2021 to September 2021, a total of 40 CHD patients who were hospitalized in the 2nd Affiliated Hospital of Harbin Medical University were enrolled using the convenient sampling method and event-related potential (ERP). Patients were divided into the type D personality group and the non-type D personality group by Type D Personality Scale-14, and matched according to age, gender and years of education. The Self-Rating Depression Scale was used to exclude patients with depression. The Negative Cognitive Processing Bias Questionnaire (NCPBQ) was used to measure negative cognitive processing bias. Dot-probe task and ERP technology were used to analyze the characteristics of attention bias, with the emotional face images as experimental stimulation materials.Results:A total of 15 CHD patients with type D personality and 15 CHD patients without type D personality were enrolled. The scores of NCPBQ and negative rumination bias dimension of CHD patients with type D personality were higher than those of CHD patients without type D personality, and the differences were statistically significant ( P<0.05). Behavioral experiments data showed that CHD patients without type D personality had a shorter response time to positive face images than negative face images in inconsistent conditional stimulation ( F=10.646, P<0.05), CHD patients without type D personality had a shorter response time to negative face images in consistent prompt type than inconsistent prompt type ( F=4.744, P<0.05). ERP experimental data showed that CHD patients with type D personality has higher N1 amplitude facing negative and positive face images stimulation ( F=8.973, P<0.05), CHD patients without type D personality had higher P3 amplitude facing positive face images stimulation ( F=6.750, P<0.05) . Conclusions:CHD patients with type D personality patients have negative cognitive processing bias, and they are more sensitive to emotional stimulation, invest more attention in the early stage of cognitive process, and have difficulty in disengaging attention from negative stimulation. Clinical medical staff should reduce negative stimulation and carry out positive psychological intervention in CHD patients with type D personality to reduce the adverse effects of negative stimulation on the prognosis of patients.

Objective:To explore the effects of mindfulness-based stress reduction training (MBSRT) on anxiety and depression levels and sleep quality of clinical nurses.Methods:Totally 90 clinical nurses from the Second Hospital of Harbin Medical University were selected by convenient sampling from April 2018 to June 2019, and were divided into experimental and control groups according to the order of enrollment, with 45 cases in each group. Nurses in the control group received routine sleep and emotional guidance, while nurses in the experimental group underwent MBSR training on this basis. Self-Anxiety Scale (SAS) , Self-Depression Scale (SDS) , and Pittsburgh Sleep Quality Index (PSQI) were used to evaluate the intervention effect in both groups 1 day before the intervention and the day when the intervention was completed. Finally, 44 nurses in the experiment group and 45 in the control group completed the study.Results:Before the MBSRT intervention, there was no statistically significant difference in the SAS, SDS, and PSQI scores between the two groups of nurses ( P>0.05) . After the intervention, the SAS and SDS scores of nurses in the experimental group were lower than those of the control group, and the differences were statistically significant ( t=-3.117, -3.498; P<0.05) ; the total PSQI score and scores in such dimensions as subjective sleep quality, sleep latency, sleep continuity, sleep efficiency, sleep disorder, hypnotic drug use, and daytime dysfunction in the experimental group were lower than those of the control group, and all differences were statistically significant ( P<0.05) . Conclusions:MBSRT can effectively reduce the level of anxiety and depression and improve the sleep quality of clinical nurses.

Objective To develop a nurse medication unsafe behavior scale, so as to provide an effective tool for evaluating nurses' unsafe behaviors and provide scientific basis for the safe medication management of clinical nurses. Methods From July to August 2016, a semi-structured interview was conducted among 5 nursing managers and 5 clinical nurses from an affiliated hospital of a medical university in Harbin city selected by purposive sampling method. The initial scale was formed by analyzing semi-structured interview data, combining with relevant literature. From November to December 2016, a pre-test was conducted among 380 nurses from an affiliated hospital of a medical university in Harbin city, who met the inclusion criteria, selected by convenience sampling method, by pre-test version of the scale. A large sample test scale was formed by expert review and pre-test. From January to February 2017, a total of 1 243 clinical nurses from 2 medical university affiliated hospitals, 2 provincial class Ⅲ grade A hospitals and 1 municipal class Ⅲ grade A hospital were selected by convenience sampling method for a large sample test, and the reliability and validity of the scale were tested to form the final scale. Results The nurse medication unsafe behavior scale consisted of 4 factors (treatment of medical orders, medication preservation and dispensing, medication administration and medication monitoring) and 25 items. The cumulative variance contribution rate was 60.897%. Confirmatory factor analysis showed that the fit indexes χ2/df, GFI, CFI, IFI and RMSEA were 2.872, 0.922, 0.951, 0.951 and 0.050 respectively. The content validity index was 0.96. The Cronbach's α coefficient and split half were 0.935 and 0.841 respectively. Conclusions The nurse medication unsafe behavior scale has good reliability and validity. It can be used as a valid tool to measure nurse medication unsafe behavior.

Type D personality plays an important role in the occurrence and development of coronary heart disease. This review interpreted the relationship and mechanism between type D personality and coronary heart disease and confirmed that the type D personality was an important risk factor for coronary heart disease. It benefits to early screening of personality in coronary heart disease patients and provides more specifically clinical treatment and nursing interventions for coronary heart disease patients, thus moves forward the check point of prevention and treatment for coronary heart disease.