Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To understand the use of the corresponding scenarios of digital transformation of"convenient medical service"in Shanghai municipal hospital outpatient department from the perspective of patients,and to provide relevant suggestions for promoting digital transformation.Methods A questionnaire survey was conducted among outpatients in Shanghai municipal hospitals to understand the use of digital transformation scenarios and their cognition of digital transformation,and the hypothesis was verified from the perspectives of technology,environment and social factors through the Technology Acceptance Model.Results A total of 4 285 valid questionnaires were collected.The top three scenarios used in outpatient digital transformation are"Precise Appointment"(71.18％),"Convenient Payment"(67.47％)and"Electronic Ticket"(48.87％).Patients'positive experience of friendly interface,stable system,recommendations from relatives and friends and social media significantly and positively influenced their perceptions of technical usability and usefulness.Positive feelings of technical usability and usefulness also encouraged patients to accept and promote digital transformation more actively(P＜0.001).Conclusion The use scenario of patients'outpatient digital transformation is closer to the core medical treatment process.The construction of relevant digital scenarios requires the joint promotion of medical institutions and society.The age-appropriate process transformation for elderly patients needs to reflect humanistic care.

Objective To investigate the effect of silenced RBM8A gene on the biological behavior (proliferation, migration, and apoptosis) of human endometrial cancer HEC-1A cells and its possible mechanism. Methods The hairpin shRNA targeted by the RBM8A gene was designed, and the best shRNA silencing fragment was screened. The recombinant lentiviral interference vector carrying the target gene was constructed and used to infect HEC-1A cells. Cells with stable knockdown of RBM8A gene were screened by puromycin as the experimental group (shRBM8A), while the shRNA of nonsense sequence was designed as the control group (shControl). CCK-8 method was used to detect cell proliferation, and flow cytometry was used to detect cell apoptosis. Transwell assay was used to detect cell migration and invasion. Western blot was used to analyze the expression of apoptosis-related proteins and EMT signal transduction pathway related proteins. Results In comparison with the shControl group, after RBM8A knockdown, HEC-1A cell proliferation was reduced, apoptosis was increased, migration and invasion ability were significantly inhibited (P < 0.05), the expression of apoptosis-related proteins cleaved caspase 9 and caspase 3 increased, EMT-related protein E-cadherin expression increased, and Vimentin expression decreased. Conclusion RBM8A gene silencing can inhibit the proliferation, migration, and invasion and promote the apoptosis of endometrial cancer cells. The inhibition of EMT signal transduction pathway may be its mechanism.

Objective:To report the sampling study design and radiography protocol of a large-sample investigation on skeletal maturation of 3 to 18-year-old children in China.Methods:Multi-stage stratified random sampling was employed in this study. Two provinces, municipalities, or autonomous regions were randomly selected from each of the seven regions of China, including Northeast China, Northwest China, North China, Central China, East China, Southwest China, and South China. Then one rural and one urban investigation site were randomly selected from each province, municipality, or autonomous region. In total 28 sites were included. Among those sites, four residential districts were randomly selected from each urban site, and four townships from each rural site. For each residential district or township, 1-4 kindergartens, primary schools, and middle schools were chosen. Random cluster sampling was used to extract 3-<6-year-old children in kindergartens, and 6-18-year-old children in primary schools and middle schools. The investigation on skeletal maturation was sampled proportionate to the sampling of the whole study. The estimated simple size was 780 for each site, and 21 840 for all 28 sites in total. There were six groups of 3-<6-year-old children classified at 0.5-year intervals, and 12 groups of 6-18-year-old children classified at 1-year intervals. Posteroanterior position radiography of the left hand and wrist was achieved for all subjects.Results:The study was performed from August 26, 2019 to October 16, 2021. In total, 20 444 children received posteroanterior position radiography of the left hand and wrist, including 10 196 males and 10 248 females, 9 711 urban and 10 733 rural, respectively. The 3-<6-year-old group included 1 611 (male 819, female 792) subjects, and the 6 to 18-year-old group included 18 833 (male 9 377, female 9 456) subjects.Conclusion:This nationwide investigation on skeletal maturation of 3 to 18-year-old children in seven regions of China was successfully preformed. The results of this study can provide an important reference for establishing the current evaluation criteria of bone age in Chinese children and adolescents.