The glasses-free three dimensional(3D) endoscopic display system provides the surgeon with the depth information of the minimally invasive surgery scene obtained from the binocular perspective, which can effectively relieve the surgeon's posture fatigue and visual fatigue during the long-term surgery, and assist in the operation of surgical instruments more accurately to reduce the damage to the surrounding tissues of the operation area. However, the glasses-free 3D display device currently has the problem of a narrow optimal viewing zone and easy crosstalk, especially in the surgical teaching application scenario, which performs poorly. In order to overcome the limitation of the narrower field of view, we introduce deep learning algorithms to detect and locate multiple faces, fine-tune the 3D display grating of the endoscope, rearrange pixels, and change the best view area, so that more people can get the best view. The experimental results show that the face detection accuracy of the method is 97.88%, and the detection time is 135 frames/ms, which achieves high accuracy while maintaining real-time performance.
Endoscopes ; Endoscopy ; Humans ; Imaging, Three-Dimensional ; Minimally Invasive Surgical Procedures ; Surgical Instruments

Objective:To investigate the reversal multidrug resistance effects of curcumin on human colorectal cancer cell lines resistant to oxaliplatin( SW620/OxR) and whether its mechanism was involved in downregulation of STAT3 signaling.Methods: The IC50 value(50%cell growth inhibitory concentrations) of curcumin to SW620/OxR cell lines was obtained by WST-1 reagent,which was one kind of cell proliferation assay.Later experiments included in the experimental group and the control group.In the experimental group,SW620/OxR cell lines were exposed to the previous experiment IC50 concentrations of curcumin plus 2 μmol/L oxaliplatin for 48 h,while in the control group,SW620/OxR cell lines were cultured in medium containing in 2μmol/L oxaliplatin.In the two groups:apoptosis was detected by flow cytometry;the protein expression levels of phosphorylated STAT3(P-STAT3) and P-gp were disclosed by Western blot.Results:The IC50 value of curcumin to SW620/OxR cell lines was 18.9 μmol/L.The apoptosis rate of cells in the control group and the experimental group were respectively ( 5.08 ±1.82 )% and ( 30.69 ±2.94 )%, the apoptosis rate of the experimental group was significantly higher than that of the control group ( P<0.05 ) .The protein expression levels of phosphorylated STAT3(P-STAT3) and P-gp in the experimental group was significantly lower than that in the control group(P<0.05).Conclusion:Curcumin can reverse drug resistance in colorectal cancer cell lines resistant to oxaliplatin, its effect may be associated with downregulation of STAT3 signaling pathways.