Ultra-performance liquid chromatography-quadrupole-electrostatic field Orbitrap mass spectrometry(UHPLC-Q-Exactive Orbitrap MS/MS) was used for rapid identification of the chemical components in Kaixin San substance benchmark. The gradient elution was performed through a Waters ACQUITY~(TM) BEH C_(18) column(2.1 mm×150 mm, 1.7 μm) with water-acetonitrile as mobile phase, a column temperature of 30 ℃, a flow rate of 0.3 mL·min~(-1), and a sample size of 1 μL. The scanning was performed in the negative ion mode. The complex component groups in Kaixin San substance benchmark were quickly and accurately identified and clearly assigned based on the comparison of the retention time and MS data with those of the reference substance as well as the relative molecular weight of the same or similar components in the mass spectrum database and literature. A total of 77 compounds were identified, including 26 saponins, 13 triterpenoid acids, 20 oligosaccharide esters, 5 xanthones, and 13 other compounds. The qualitative method established in this study can systematically, accurately, and quickly identify the chemical components in Kaixin San substance benchmark, which can provide a basis for the further analysis of its active components in vivo and the establishment of its quality control system.
Benchmarking ; Chromatography, High Pressure Liquid/methods* ; Drugs, Chinese Herbal/chemistry* ; Tandem Mass Spectrometry/methods*

OBJECTIVE：To stud y the effects of different processed products of Whitmania pigra on hemorheology and coagulation indexes in acute blood stasis model rats. METHODS ：SD rats were randomly divided into blank group ，model group ， aspirin group ，W. pigra hang-dried product low- ，medium- and high-dose groups ，W. pigra talcum powder-ironed product low- ， medium- and high-dose groups ，W. pigra wine bran-processed product low- ，medium- and high-dose groups ，with 6 rats in each group. Except for blank group ，other groups received subcutaneous injection of epinephrine hydrochloride and ice water bath for 15 d to induce acute blood stasis model. From the 8th day of modeling ，rats in aspirin group were given aspirin 0.2 g/kg intragastrically. Rats in each dose group of W. pigra processed products were given relevant medicine 0.35，1.4，3.5 g/kg intragastrically（calculated by crude drug ）. Rats in blank group and model group were given constant volume of normal saline intragastrically， once a day ， for consecutive 8 days. Hemorheology indexes as whole blood viscosity （high， medium and low shearrate ），plasma viscosity ，erythrocyte com deformation index ，erythrocyte aggregation index ，hematocrit， and blood coagulation indexes as prothrombin time （PT）， mail：wcl19960125@163.com activated partial prothrombin time （APTT），thrombin time （TT）were determined. RESU LTS：Compared with blank group ，whole blood viscosity under different shear rates ，plasma viscosity ， erythrocyte aggregation index and hematocrit of model group were increased significantly ，while erythrocyte deformation index was significantly decreased ，PT，TT and APTT were significantly shortened （P＜0.01）. Compared with model group ，whole blood viscosity under different shear rates ，plasma viscosity ，erythrocyte aggregation index and hematocrit of aspirin group and W. pigra hang-dried product ，talcum powder-ironed product ，wine bran-processed product high-dose groups were decreased significantly ， while erythrocyte deformation index were significantly increased ，and PT （only W. pigra talcum powder-ironed products high-dose group），APTT（except for W. pigra hang-dried products high-dose group ）and TT were prolonged significantly. The whole blood viscosity of W. pigra hang-dried product medium-dose group under low shear rate ，and those of W. pigra talcum powder-ironed product low-dose ，wine bran-processed product medium-dose groups under low and medium shear rates were decreased significantly. Erythrocyte deformation index of W. pigra talcum powder-ironed product medium-dose group was increased significantly ，while erythrocyte aggregation index was decreased significantly ，and PT ，TT were prolonged significantly. APTT of W. pigra hang-dried product medium-dose group was prolonged significantly. Hematocrit of W. pigra wine bran-processed product low-dose group was decreased significantly （P＜0.05 or P＜0.01）. CONCLUSIONS ： W. pigra hang-dried， talcum powder-ironed and wine bran-processed product can effectively improve hemorheology indexes and prolong blood coagulation time.

Objective To investigate the application method and effect of Levin's conservation model in nursing intervention for patients with cancer-related fatigue (CRF). Methods Totally 64 patients admitted into the Department of Medical Oncology, Qingdao Tumor Hospital from November 2015 to May 2016 were selected and divided into an observation group (32 patients) and a control group (32 patients) by random sampling. Patients in the control group received routine nursing intervention, while patients in the observation group received nursing intervention under Levin's conservation model. The effect was evaluated with the simplified and revised fatigue scale, quality of life questionnaire and Pittsburgh sleep quality index (PSQI). Results After the intervention, the patients in the observation group showed a lower degree of fatigue than the patients in the control group (Z=-2.230,P< 0.05). In the evaluation of the quality of life, the patients in the observation group had higher scores in all dimensions than the patients in the control group (t=13.520, 14.630, 8.561, 11.983, 12.645; P< 0.05). The sleep quality index of the patients in the observation group was (4.9±0.8), lower than that of the patients in the control group [(8.5±0.9)] (t=-17.194,P< 0.05). Conclusions Levin's conservation model makes the development of nursing measures more targeted and practical, and it is worthy to be promoted and applied in clinical nursing.

Objective To investigate the effects of group psychotherapy on perceived stigma and quality of life and to provide reference for targeted psychological interventions in patients with lung cancer. Methods Totally 90 patients with lung cancer who attended subsequent visits in a Class Ⅲ Grade A hospital from January to December 2017 were selected by convenient sampling and divided into control group (n=45) and observation group (n=45) according to the random number table. The control group received conventional follow-up visits, while the observation group received group psychotherapy on this basis. The perceived stigma and quality of life score before and after intervention were compared between the two groups.Results The perceived stigma score was (58.33±8.96) in the observation group, which was better than that post intervention (t=6.043, P<0.01). The perceived stigma score post intervention was better in the observation group than in the control group (t=2.145,P=0.035). The observation group showed higher scores in role function, emotional function, social function and overall quality of life while lower scores in fatigue and sleep disorder than the control group (t=-2.304, -2.444, -2.434, 2.426, -2.827, 2.032;P<0.05).Conclusions Group psychotherapy can mitigate the perceived stigma and improve their quality of life in patients with lung cancer, thereby contributing to the recovery of their physical and psychological rehabilitation. Therefore, it is worth promoting in clinical work.