Objective To evaluate the association between calpain-10 gene polymorphism and type 2 diabetes mellitus(T2DM) by system assessment method.Methods CNKI,CBM and WangFang Data were searched for studies assessing the genetic association between calpain-10 gene polymorphism and T2DM.The data from included studies were extracted to calculate for odds ratio (OR).The simplified version of STREGA list was used to evaluate the quality of researches.Review Manger 5.3.1 software was used to analyze the heterogeneity (I2 values) among the studies,α=0.05.Results A total of 21 randomized controlled studies were analyzed.3062 T2DM patients were involved in SNP43 loci polymorphism evaluation.The highest score of STREGA list was 6 points and the lowest score was 3 points.The proportions of 6 points,5 points,4 points and 3 points were 38.10％,33.33％,9.52％ and 19.05％,respectively.Both scores of "whether having description of genotyping method" item and "whether having sufficient data" item were 0 point.Meta analysis results showed that SNP 43 mutation type "A" of Calpain-10 gene was related to T2DM (OR 0.60,95％CI 0.52,0.68,P＜0.05).The ORs of SNP 19,44 and 63 for T2DM were 0.92,1.50 and 0.84,with P values 0.50,0.11 and 0.08,respectively.The sensitivity analysis showed the similar results,with a small publication bias.Conclusion SNP43 loci of Calpain-10 gene has a genetic association with T2DM.