Objective:To investigate the value of peripheral blood eosinophils (EOS) in predicting the therapeutic effect of sublingual specific immunotherapy (SLIT) in patients with allergic rhinitis (AR).Methods:From January 2018 to June 2020, 200 patients with AR who received SLIT in Dongguan Kanghua Hospital were evaluated for their symptoms before treatment and 6 months after treatment. The curative effect was evaluated according to the improvement of the total symptom score. The number of eosinophils, percentage of eosinophils in peripheral blood were measured before treatment. The correlation between curative effect and EOS count and EOS percentage was analyzed and the value of these two indicators in predicting the efficacy of SLIT was evaluated.Results:Of the 200 AR patients, 178 patients were confirmed to have completed the treatment course of 6months. 126 effective and 52 ineffective, the effective rates were 70.8%. There was a weak correlation between the number of EOS in peripheral blood and the percentage of EOS in peripheral blood before treatment and the efficacy of SLIT ( r=0.168, P=0.048; r=0.250, P=0.001). The area under the curve of peripheral blood EOS count was 0.380, and the percentage of peripheral blood EOS was 0.355, which were all less than 0.5. Conclusions:There was no significant correlation between the number of peripheral blood EOS count and peripheral blood EOS percentage and the efficacy of SLIT , therefore, both of blood EOS count and blood EOS percentage have no value in predicting the efficacy of SLIT.

Objective:To investigate the effects of exogenous nitric oxide (NO) on radiation response of nasopharyngeal carcinoma 5-8F radiotherapy resistant cell line (5-8FRs) and to provide experimental basis for finding suitable radiosensitizer on nasopharyngeal carcinoma.Methods:5-8FRs cells were cultured in vivo and treated with sodium nitroprusside (SNP) of different concentrations. The inhibition rate of cell proliferation was detected by cell counting kit-8 (CCK8) method. A concentration of IC 01 SNP (1% SNP) which had no obvious effect on the proliferation of 5-8FRs cells was screened out. The 5-8FRs cells were intervened with 1, 2, 4, 6 Gy and 8 Gy radiation to determine the radiation dose of IC 15 (15% radiation dose). 5-8FRs cells were treated with IC 01 SNP concentration, IC 15 radiation dose and radiotherapy alone or in combination. The morphological changes of 5-8FRs cells were observed under microscope. The proliferation inhibition rate of each group was detected by CCK-8 method, the apoptosis was detected by flow cytometry, and the concentration of NO in cell supernatant was detected by nitrate reduction method. Results:⑴ The proliferation of 5-8FRs had been inhibited in a concentration-dependent manner by SNP and a dose-dependent manner by radiation. The SNP concentration of IC 01 was (513.89±14.69)μmol/L (SNP group). The radiation dose of IC 15 was (3.96±0.33)Gy (radiotherapy group); ⑵ Compared with single SNP group and radiotherapy group, the morphology of 5-8FRs cells in combination group (SNP+ radiotherapy) was significantly different, floating cells increased significantly, the number of adherent cells gradually decreased and lost their original morphology; ⑶ SNP concentration of IC 01 had no significant effect on the proliferation of 5-8FRs cells, but the inhibition rate of combined group was significantly higher than that of radiotherapy group ( t=7.708, P<0.01); The concentration of NO in the combined group was significantly higher than that in the single radiotherapy group [(310.03±5.76)μmol/L vs (77.34±2.60)μmol/L, P<0.05]; ⑷ The spontaneous apoptosis rate of 5-8FRs was (1.35±0.06)%, while the apoptosis rate in the group of IC 01SNP was (2.22±0.37)%, with no significant difference. The apoptosis rate of 5-8FRs in the combined group (50.27±2.24)% was significantly higher than that of the group of IC 15 radiation dose(15.37±0.65)%. Conclusions:Under no obvious toxicity to cells themselves circumstances, exogenous nitric oxide with appropriate concentration could significantly enhance the radiosensitivity on 5-8FRs.

Objective:To investigate the clinical effect of modified atlantooccipital decompression combined with occipitocervical internal fixation on Chiari type I malformation combined with syringomyelia and atlantoaxial dislocation.Methods:Twenty-five patients with Chiari I malformation combined with syringomyelia and atlantoaxial dislocation accepted by modified atlantooccipital decompression combined with occipitocervical internal fixation in our hospital from January 2011 to March 2019. The clinical data of these patients were retrospectively analyzed. The changes of peak velocity of cerebrospinal fluid in the dorsal part of the spinal cord, electrophysiological results, atlantodental interval (ADI) values, sizes of syringomyelia, and Chicago Chiari outcome scale (CCOS) scores before and after operation were compared.Results:The peak velocity of cerebrospinal fluid in the dorsal spinal cord after surgery ([3.25±0.47] cm/s) was statistically higher in these patients than that before surgery ([2.13±0.19] cm/s, P<0.05). As compared with the preoperative results, ADI values, sizes of syringomyelia, and proportion of patients with abnormal electrophysiological monitoring at 6 months after surgery were significantly decreased, and CCOS scores at 6 months after surgery were significantly increased ( P<0.05). There were no new nerve function damage, infection, cerebrospinal fluid leakage, paralysis, respiratory failure or death. Conclusions:Modified atlantooccipital decompression combined with occipitocervical internal fixation plays effective role in atlantooccipital decompression and atlantoaxial anatomical reduction in patients with Chiari malformation type I combined with syringomyelia and atlantoaxial dislocation. The remission rate of syringomyelia is high, the symptoms and signs improve obviously, and the postoperative complications are less.

Objective To investigate the effect induced by specific inducible nitric oxide synthase (iNOS) inhibitor 1 400 W on nasopharyngeal carcinoma CNE-2 cells lines and its mechanism.Methods CNE-2 cells were treated by different concentrations of 1 400 W,diamminedichloroplatinum (DDP),and both chemicals.Cell counting kit-8 (CCK-8) was used to examine the viability of cells.Quantitative realtime polymerase chain reaction (qRT-PCR) was applied to detect the iNOS mRNA expression.Results The expression of iNOS mRNA was down-regulated by 1400W and was positively correlated with inhibitionrate of cell proliferation.1 400 W inhibits proliferation of CNE-2 cell in a concentration-dependent manner.The proliferation inhibition rate of CNE-2 cells treated by 1 400 W combined with DDP was not enhanced.Conclusions Specific inducible nitric oxide synthase inhibitor 1 400 W can exerts anti-tumor effect though inhibiting the expression of iNOS mRNA;The mechanism of chemosensitization induced by iNOS inhibitor on CNE-2 cells may be closely related level of down-regulation of iNOS expression.

Actinomycosis ; complications ; Humans ; Laryngeal Neoplasms ; surgery ; Papilloma ; surgery ; Postoperative Complications ; microbiology

The chemosensitivity enhancive mechanism on nitric oxide includes three routes:adjust the expression level of hypoxia-inducible factor-1 α(HIF-1 α),classical nitric oxide signal pathway and combine with the reaction products of other molecules.Traditional nitric oxide donor compounds include nitroferricya-nide,organic nitrates,s-nitrosothiols,azo onium diol aldoxide,non-steroids anti-inflammatory drugs,whose function of antitumor and chemotherapy sensitization caused extensive attention.

A 61 year-old male patient, plasma fibrinogen level was 2.98 g/L, endoscopic sinus surgery was performed under general anesthesia for polypoid of uncinate process with mycotic maxillary sinusitis. Hemocoagulase were given in pre- and post-operative for treatment. The patient was found postoperative drain blood continuously since 3 days after surgery, when the dose of hemocoagulase reach 26 KU, and fibrinogen determined in Plasma was 0.48 g/L. Coagulation returned to normal and nasal bleeding stopped after discontinuation of the hemocoagulase and supplement with fibrinogen.
Batroxobin ; adverse effects ; Blood Coagulation Disorders ; chemically induced ; Endoscopy ; adverse effects ; Fibrinogen ; analysis ; Humans ; Male ; Middle Aged ; Nasal Surgical Procedures ; adverse effects ; Paranasal Sinuses ; surgery

OBJECTIVE: To explore the best time of intratympanic dexamethasone injection to treat sudden sensorineural hearing loss (SSNHL) as salvage therapy so that to improve the curative efficacy on sudden deafness at the utmost. METHOD: A total of 192 patients with SSNHL were included in this study, among whom 63 cases received the systemic steroid therapy throughout the study, while the other ones were treated with systemic steroid as initial treatment and were given intratympanic steroid administration as salvage treatment starting at different time point. The salvage treatment started on the 3rd day after the beginning of the initial treatment for 29 cases, on the 7th day for 38 cases, on the 14th day for 43 cases, and 1 month later for 19 cases. All the patients were followed up for 2 months. RESULT: The recovery rates and total effective rates showed no statistically significant difference between the patients received only systemic steroid therapy and the ones received intratympanic steroid administration on the 3rd, 7th day and 1 month later after the initial treatment. The recovery rate and total effective rate exhibited statistically significant difference between the patients received intratympanic steroid administration since the 14th day after the initial treatment and the ones received only systemic steroid therapy, with the numerical value of P 0. 037 and 0. 034, respectively. CONCLUSION (1) As an initial management plan, the curative effects. between the intratympanic steroid administration and the systemic steroid therapy were not significantly different. (2) As a salvage treatment, intratympanic steroid was a better choice for patients who have not completely recover from ISSNHL after failure of initial management with systemic steroid only. (3) The best time point of salvage treatment with intratympanic steroid was about 2 weeks after initial management with systemic steroid.
Hearing Loss, Sensorineural ; drug therapy ; Hearing Loss, Sudden ; drug therapy ; Humans ; Injection, Intratympanic ; Salvage Therapy ; Steroids ; therapeutic use ; Treatment Outcome ; Tympanic Membrane

OBJECTIVE: To explore the best evaluating time of curative effect on sudden deafness so that the curative effect on sudden deafness can been evaluated more exactly and literally. METHOD: Pure tone audiometries in 112 cases of sudden deafness were performed on the pretreatment day and on the third, seventh, fourteenth post-treatment day, and in the first, second, third, fourth post-treatment month. All of acoustical data were analyzed. RESULT: The total effective rates were statistical different between the third, seventh post-treatment day and the fourteenth post-treatment day, the first, second, third, fourth post-treatment month. There were no statistical difference between the fourteenth post-treatment day and the first post-treatment month. There were statistical difference between the fourteenth post-treatment day and the second, third, fourth post-treatment month. The total effective rates were no statistical difference between the first and the second, third, fourth post-treatment month but it was fluctuated in the first post-treatment month. The total effective rates were no statistical difference between the second and the third, fourth post-treatment month and it was changeless on the second post-treatment month. From the curve of recruitment of hearing in different time, the curve of the total effective rates ascend from the third post-treatment day, then get to plateau from the second post-treatment month. If the cure rates, the efficiency rates, the effective rates in different time were analyzed, respectively, the hearing improvement ascend in first two weeks then. Hearing improvement get to plateau from fourteenth post-treatment day. CONCLUSION (1) The evaluated results of curative effect to sudden deafness correlated vary in different time point post-treatment. (2) Prognosis can be predicted approximately 2 weeks after treatment. Patients who recover acoustic sensibility within 2 weeks have more significant improvement than the patients who hearing improvement after 2 weeks treatment. (3) The results of curative effect are stable after 2 months. If the data of this time were adopted, it would be more objective and accurate to evaluate the curative effect on sudden deafness.
Adolescent ; Adult ; Female ; Hearing Loss, Sudden ; diagnosis ; therapy ; Humans ; Male ; Middle Aged ; Prognosis ; Time Factors ; Treatment Outcome ; Young Adult

OBJECTIVE: To investigate the effect of hyperbaric oxygen therapy to different degrees of hearing loss and different types of threshold curve in sudden deafness patients, and establish a personalized therapy, so as to promote clinical effect to sudden deafness. METHOD: One hundred and twenty cases were divided into two groups respectively (each group 60 cases). Drug treatment had been used in group A for two weeks. The patients in group B received drug treatment combined with hyperbaric oxygen therapy for two weeks. According to the different degrees of hearing loss and the types of audiogram in two groups, the statistical analysis to the difference of hyperbaric oxygen therapeutic effect was done. RESULT: The effective rates was statistically different (chi2 = 8.044, P < 0.01) between group A (60.00%, 36/60) and group B (83.33%, 50/60). There was no statistical difference between two treatment program in mild and the profound deafness patients. The statistical difference could been found between moderate and the severe deafness, the value of chi2 and p were 4.969, 5.560 and 0.040, 0.035, respectively. In group A, the statistical difference could only been found between mild and the profound deafness patients (chi2 = 6.739, P < 0.05). In group B, the statistical difference could been found in mild and the profound deafness, the moderate and the profound deafness, the severe and the profound deafness patients. The value of chi2 were 11.500, 15.058,10.221 and P < 0.01 respectively. The effective rates was no statistical difference in two treatment programs in the upsloping audiogram and the profound deafness. The statistical difference could been found between the downsloping and the flat audiogram, with the value of chi2 and p were 5.144 and 5.969, P < 0.05, respectively. In group A, the statistical difference could been found between the ascending and descending audiogram, the ascending and the flat audiogram,the ascending type and the profound deafness. The value of chi2 were 6.188, 7.247, 8.082 and P < 0.05, respectively. No statistical difference could been found among the other types of audiogram. In group B, no statistical difference could been found among all types of audiogram. CONCLUSION The effect of hyperbaric oxygen therapy varies with degree of hearing loss and audiogram type. Drug treatment combined with HBOT can promote clinical effect for patients in moderate deafness and severe deafness, the descending and flat type of audiogram.
Adolescent ; Adult ; Female ; Hearing Loss ; classification ; therapy ; Hearing Tests ; Humans ; Hyperbaric Oxygenation ; Male ; Middle Aged ; Oxygen ; therapeutic use ; Treatment Outcome ; Young Adult