Objective: To investigate the HCV genotypes distribution of 107 HIV/HCV co-infection individuals in Hunan province. Methods: HCV antibodies positive plasma samples were collected from HIV-1 antibodies positive plasma bank in Hunan Provincial Center for Disease Control and Prevention and carried out the nucleic acid amplification, the PCR products were used for the HCV genotypes analysis. Results: For 107 samples, 77.5%(83/107) were from intravenous drug users and 25.2%(27/107) were underwent spontaneous clearance of HCV. The HCV genotypes distribution varied greatly in different routes of HIV infection. The non-1/non-2 types exceeded 70% in intravenous drug use and unknown route of transmitted infection populations, while the types 1 and 2 mainly occurred in blood transfusion or aphaeresis plasma populations, accounted for 83.3%. There was no statistical significant differences of immune and liver function among the three groups. Conclusions Among 107 HIV/HCV co-infection individuals in Hunan province, most of them were intravenous drug users and the distribution of HCV genotypes varied greatly in different routes of HIV infection.