AIM To observe the protective effects of Qigu Capsule-medicated serum on C2C12 myotube cell atrophy induced by dexamethasone.METHODS The Qigu Capsule-medicated serum was prepared.C2C12 cells cultured in vitro were divided into the normal control group,the dexamethasone group,the 10%blank serum group and the 10%Qigu Capsule-medicated serum group for 48 h,corresponding drug treatment,followed by 24 h culture with 10 μmol/L dexamethasone except the control group,and had cell viability detection by CCK-8 method.With their myotube cells intervened accordingly by the aforementioned regime following 6-7 days differentiation with 2%horse serum induction,the C2C12 cells had their myotube cells diameter measured;and the expressions of proteins related to MyHC,MuRF-1,Atrogin-1 and PI3K/Akt signaling pathway detected by Western blot.The impact of PI3K inhibitor LY294002 on the diameter of myotube cells and the expression of MuRF-1,Atorgin-1 and PI3K/Akt signaling pathway related proteins were detected as well.RESULTS Compared with the normal control group,the dexamethasone group and the blank serum group were observed with decreased viability of C2C12 cells(P<0.01),decreased diameter of myotube cells(P<0.01),increased protein expressions of MuRF-1 and Atrogin-1(P<0.01),and decreased expression of MyHC protein and phosphorylation levels of PI3K,Akt,mTOR and FoxO3a(P<0.01).Compared with the dexamethasone group,the Qigu Capsule-medicated serum group showed increased viability of C2C12 cells(P<0.01);increased diameter of myotube cells(P<0.01);decreased protein expressions of MuRF-1 and Atrogin-1(P<0.01);and increased expression of MyHC protein and phosphorylation levels of PI3K,Akt,mTOR and FoxO3a(P<0.05,P<0.01).The intervention of LY294002 upon the Qigu Capsule-medicated serum group offset the anti-muscular tube atrophy effect(P<0.01),increased the protein expressions of MuRF-1 and Atorgin-1(P<0.01),and decreased the phosphorylation levels of PI3K,Akt,mTOR and FoxO3a(P<0.05,P<0.01).CONCLUSION The Qigu Capsule-medicated serum can alleviate the atrophy of C2C12 myotubes induced by dexamethasone,and its mechanism may be related to the activation of PI3K/Akt signaling pathway.

OBJECTIVES: To investigate the incidence of extrauterine growth retardation (EUGR) and its risk factors in very preterm infants (VPIs) during hospitalization in China. METHODS: A prospective multicenter study was performed on the medical data of 2 514 VPIs who were hospitalized in the department of neonatology in 28 hospitals from 7 areas of China between September 2019 and December 2020. According to the presence or absence of EUGR based on the evaluation of body weight at the corrected gestational age of 36 weeks or at discharge, the VPIs were classified to two groups: EUGR group (n=1 189) and non-EUGR (n=1 325). The clinical features were compared between the two groups, and the incidence of EUGR and risk factors for EUGR were examined. RESULTS: The incidence of EUGR was 47.30% (1 189/2 514) evaluated by weight. The multivariate logistic regression analysis showed that higher weight growth velocity after regaining birth weight and higher cumulative calorie intake during the first week of hospitalization were protective factors against EUGR (P<0.05), while small-for-gestational-age birth, prolonged time to the initiation of total enteral feeding, prolonged cumulative fasting time, lower breast milk intake before starting human milk fortifiers, prolonged time to the initiation of full fortified feeding, and moderate-to-severe bronchopulmonary dysplasia were risk factors for EUGR (P<0.05). CONCLUSIONS It is crucial to reduce the incidence of EUGR by achieving total enteral feeding as early as possible, strengthening breastfeeding, increasing calorie intake in the first week after birth, improving the velocity of weight gain, and preventing moderate-severe bronchopulmonary dysplasia in VPIs.
Female ; Fetal Growth Retardation ; Gestational Age ; Hospitalization ; Humans ; Incidence ; Infant ; Infant, Newborn ; Infant, Premature ; Infant, Very Low Birth Weight ; Prospective Studies ; Risk Factors

Wnt5a is a representative Wnt ligand that regulates multiple cellular functions through the Wnt5a non-classical pathway. Although Wnt5a has been implicated in various pathological conditions, its role in cancer is ambiguous and might involve methyl modifications, distinct mRNA isoforms, as well as different downstream pathways. Therefore, it is an essential factor in cancers' progression (invasion, migration, proliferation, and epithelial-mesenchymal transition), and a potential biomarker for prognosis and treatment.

Objective To systematically evaluate the compliance and correct rate of hand hygiene(HH) among doctors and nurses in secondary and above hospitals in mainland China,and provide data support for HH promotion.Methods Literatures about study on HH in mainland China after 2015 were retrieved from Chinese and English databases,researchers evaluated the literatures according to the relevant standards,screened the qualified literatures and extracted the data strictly according to the inclusion and exclusion criteria,Meta analysis was adopted to analyze the compliance and the correct rate of HH of doctors and nurses in different levels of hospitals and at different departments.Results The overall compliance rate of HH of nurses was higher than doctors,compliance rates of HH of doctors and nurses were 55.9％ (95 ％CI:45.9％,65.8％) and 66.0％ (95％CI:58.8％,73.1％) respectively;HH correct rates of doctors and nurses were 65.2％ (95％CI:53.6％,76.9％) and 67.9％(95％CI:56.9％,78.9％) respectively.Compliance rates of HH of doctors and nurses in tertiary hospitals were both higher than secondary hospitals,compliance and correct rates of HH of doctors and nurses in intensive care unit were both higher than general departments.Conclusion The compliance and correct rate of HH in mainland China need to be further improved,especially the promotion in primary hospitals and general departments.

Objective To investigate the occurrence of healthcare-associated infection(HAI)in different weight groups of neonates in neonatal intensive care unit (NICU)and general neonatal wards,and find out the high risk factors for HAI.Methods Targeted monitoring on hospitalized neonates in the neonatal department of a hospital in 2016 and January-November 2017 was prospectively surveyed,HAI rate,device usage rate and device-associated in-fection rate in different weight groups of neonates in NICU and general neonatal wards were compared.Results From 2016 to November 2017,a total of 3 872 neonates were monitored,the total hospitalization days were 42 427 days,56 patients had 62 cases of infection,HAI rate and HAI case rate were 1.45% and 1.60% respectively.HAI rate and HAI case rate in NICU were 5.28% and 6.03% respectively;HAI rate and HAI case rate in general neo-natal wards were both 0.46%,constituent ratio of septicemia ranked first of infection in both NICU and general neonatal wards,accounting for 43.75% and 50.00% respectively,followed by lower respiratory tract(LRT)infec-tion,accounting for 27.08% and 21.43% respectively.HAI rate of different weight groups of neonates in NICU and general wards were both significantly different(χ2＝107.78,46.65,respectively,both P＜0.0001),the lower weight of the neonates,the higher HAI rate;ventilator usage rate in neonates was positively correlated with HAI (r＝0.973,P＝0.027),the higher device usage rate,the higher HAI rate.Fungal septicemia rate per 1 000 days in rainy season was higher than that of non-rainy season (0.53‰ vs 0.37‰).A total of 70 specimens were detected, 45 strains of bacteria and fungi were isolated,pathogens were all from LRT and blood system,Pseudomonas aeru-ginosa was the main pathogen from LRT,Klebsiella pneumoniae and Candida krusei were the main pathogens from blood system.Conclusion Targeted monitoring on neonatal HAI can timely find out high risk factors for neonatal HAI,analyze the causes of infection,formulate corresponding prevention and control measures,reduce HAI rate, and ensure the medical safety of neonates.

Objective To discuss the battery management of ECG monitor to enhance its benefits.Methods The application of ECG monitor battery in all the departments was analyzed,and a management scheme was proposed that executed proper supplying in the nurse station and centralized allocation in leasing center.Results The proposed scheme decreased the failure risks of the battery and saved the cost of the departments.Conclusion Battery management concept can be applied to other types of medical equipment so as to further optimize medical equipment management mechanism in the medical facility.

Objective To design a data acquisition and monitoring system to facilitate the maintenance of the ventilator.Methods The system was composed of two modules of data acquisition and monitoring.The data acquisition module used MSP430F149 SCM as the master control chip to realize real-time acquisition and storage of operation data,and the monitoring module adopted STM32 SCM as the master control chip to implement equipment monitoring,server serial port conversion and data communication.Wireless data transmission between the two modules was executed with wireless NRF24L01 module.Results The system realized real-time acquisition and storage of the ventilator log,and transmitted acquired data to the server.Conclusion The system eliminates the deficiencies of manual operation and implements basic risk analysis,and facilitates the engineer to monitor and maintain the ventilator.

AIM:To investigate the expression of serine-arginine-rich splicing factor 9/serine-arginine-rich protein 30c (SRSF9/SRp30c) and glucocorticoid receptor β(GRβ) in the glioma cells and the relationship of them. METHODS:Small interfering RNA ( siRNA) was used to knock down the expression of SRSF9 in the U87 cells.Short hairpin RNA ( shRNA) derived from lentivirus was used to establish U 87 stable knockdown cell line .Fluorescence micros-copy was used to observe and detect transfection efficiency .The expression of Grβand SRSF9/SRp30c at mRNA and pro-tein levels was determined by RT-qPCR and Western blot .The cell viability , colony formation ability and migration ability were measured by CCK-8 assay, colony formation assay and wound healing experiment .RESULTS:The mRNA and pro-tein levels of SRSF9/SRp30c and Grβin the U87 cells were both down-regulated after knockdown of SRSF9 (P<0.05). Fluorescence microscopic observation showed that a stable cell line was constructed successfully , and the transfection effi-ciency exceeded 80%.After knockdown of SRSF9 expression in the U87 cells, the cell viability and colony formation abili-ty were reduced (P<0.05).The migration ability was weakened significantly after SRSF9 was knocked down (P<0.05). CONCLUSION:SRSF9/SRp30c may promote the proliferation and migration of the glioma cells by regulating GRβ.

Objective To study a new method for fast unloading and positioning of medical shelter at night to accelerate the deployment speed of medical shelter system at night and improve the rapid reaction of medical support forces.Methods Laser marking device was used to indicate the three baseline for vehicle positioning based on three line positioning method,and then the position of the self-contained vehicle was determined.Reversing radar assisted in monitoring the vehicle position,and facilitated parking at a predetermined location.Results The problems such as low visibility were solved during the deployment at night,and the time consumed was decreased to 40 min for shelter positioning and unloading,so that the workload and artificial errors were both reduced greatly.Conclusion The method realizes medical shelter unloading efficiently with high practicability and flexibility.

ABSTRACT:OBJECTIVE To study the effects of Qingluo Tongbi Compound(QLT)on pain behavior and COX-2 mRNA ex-pression in dorsal root ganglion(DRG)and blood PGE2 concentration,and to explore the mechanisms of pain in rheumatoid ar-thritis.METHODS The mice were randomly divided into control group,celecoxib 30 mg∕kg,QLT 4.35 g∕kg,QLT 8.70 g∕kg and QLT 1 7.4 g∕kg groups.The mice pain threshold change were measured by hot plate method,and body torsion times were tested by acetic acid twisting method.The collagen-induced arthritis(CIA) was induced by collagenⅡ in DBA∕1 mice. The mice were randomly divided into control group,CIA group,celecoxib 30 mg∕kg group,QLT 8.70 g∕kg.The paws swell-ing,mechanical withdrawal threshold(MWT)and thermal withdrawal latency(TWL)were recorded.After treated with corre-sponding drugs by intragastric administration for four weeks,COX-2 mRNA in DRG and PGE2 in mice were quantified.RE-SULTS QLT reduced pain threshold and body torsion times in pain model mice(P <0.01);compared with the CIA group,the QLT group decreased the paws swelling(P <0.05),up-regulated MWT(P <0.01) and PWL(P <0.05),down-regulated the expression level of COX-2 mRNA in DRG and PGE2 concentrations in blood.CONCLUSION QLT shows certain analgesic ac-tion,which might be related to the inhibitory effect of COX-2 mRNA expression in DRG and PGE2 in blood.