The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

Pancreatic cancer is among the most malignant cancers,and thus early intervention is the key to better survival outcomes.However,no methods have been derived that can reliably identify early precursors of development into malignancy.Therefore,it is urgent to discover early molecular changes during pancreatic tumorigenesis.As aberrant glycosylation is closely associated with cancer progression,numerous efforts have been made to mine glycosylation changes as biomarkers for diagnosis;however,detailed glycoproteomic information,especially site-specific N-glycosylation changes in pancreatic cancer with and without drug treatment,needs to be further explored.Herein,we used comprehensive solid-phase chemoenzymatic glycoproteomics to analyze glycans,glycosites,and intact glycopeptides in pancreatic cancer cells and patient sera.The profiling of N-glycans in cancer cells revealed an increase in the secreted glycoproteins from the primary tumor of MIA PaCa-2 cells,whereas human sera,which contain many secreted glycoproteins,had significant changes of glycans at their specific glycosites.These results indicated the potential role for tumor-specific glycosylation as disease biomarkers.We also found that AMG-510,a small molecule inhibitor against Kirsten rat sarcoma viral oncogene homolog(KRAS)G12C mutation,profoundly reduced the glycosylation level in MIA PaCa-2 cells,suggesting that KRAS plays a role in the cellular glycosylation process,and thus glycosylation inhibition contributes to the anti-tumor effect of AMG-510.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

The development of multi-campus districts has become an important initiative for the country to expand high-quality medical resources and build a high-quality and efficient medical and healthcare service system,and is an important implementation path for the high-quality development of public hospitals.The symbiosis theory is highly applicable to multi-campus hospital development,and can provide a new perspective for the exploration of multi-campus hospital collaborative development.Through the construction of the symbiotic framework based on symbiotic unit,symbiotic interface,symbiotic environment and symbiotic model,it analyzed the symbiotic dilemma of multi-campus hospital collaborative development,and proposed the path of multi-campus hospital collaborative development,in order to provide reference for the subsequent construction and development of multi-campus hospital in China.

Objective: To compare the clinical outcomes and biomechanical characteristics of 1-, 2-, and 3-level pedicle subtraction osteotomy (PSO), and establish selection criteria based on preoperative radiographic parameters. Methods: Patients undergone PSO to treat ankylosing spondylitis from February 2009 to May 2019 in Sun Yat-sen Memorial Hospital of Sun Yat-sen University were enrolled. According to the quantity of osteotomy performed, the participants were divided into group A (1-level PSO, n = 24), group B (2-level PSO, n = 19), and group C (3-level PSO, n = 11). Clinical outcomes were assessed before surgery and at the final follow-up. Comparisons of the radiographic parameters and quality-of-life indicators were performed among and within these groups, and the selection criteria were established by regression. Finite element analysis was conducted to compare the biomechanical characteristics of the spine treated with different quantity of osteotomies under different working conditions. Results: Three-level PSO improved the sagittal parameters more significantly, but resulted in longer operative time and greater blood loss (p < 0.05). Greater stress was found in the proximal screws and proximal junction area of the vertebra in the model simulating 1-level PSO. Larger stress of screws and vertebra was observed at the distal end in the model simulating 3-level PSO. Conclusion Multilevel PSO works better for larger deformity correction than single-level PSO by allowing greater sagittal parameter correction and obtaining a better distribution of stress in the hardware construct, although with longer operation time and greater blood loss. Three-level osteotomy is recommended for the patients with preoperative of global kyphosis > 85.95°, T1 pelvic angle > 62.3°, sagittal vertical alignment > 299.55 mm, and pelvic tilt+ chin-brow vertical angle > 109.6°.