Objective:To explore the intervention effect of Shumu Peitu herb-partitioned moxibustion on clinical signs and symptoms and negative emotions of diarrhea-predominant irritable bowel syndrome (IBS-D) patients with liver-stagnation and spleen-deficiency pattern.Methods:A total of 72 patients with IBS-D of liver-stagnation and spleen-deficiency pattern treated in the Department of Gastroenterology of Nanjing Vniversity of Chinese Medicine from September 2021 to June 2022 were selected for randomized controlled trial. The patients were randomly divided into the observation group (2 cases dropped off, 34 cases in total) and control group (1 case dropped off, 35 cases in total) by random number table method. The patients in control group were treated with Tongxieyaofang (TXYF). The patients in observation group were treated with oral administration of TXYF and Shumu Peitu herb-partitioned moxibustion, and both groups were treated for 4 weeks. The clinical efficacy, Traditional Chinese Medicine (TCM) syndrome integral, IBS Quality of Life Questionnaire (IBS-QOL), IBS Symptom Severity Scale (IBS-SSS), Bristol Stool Form Scale and Hospital Anxiety and Depression Scale (HADS) were compared before and after treatment.Results:After treatment, the total effective rate of the observation group was 94.12%(32/34), which was higher than the 71.43%(25/35) in the control group, the difference was significant ( χ2 = 6.18, P<0.05). After treatment, the TCM syndrome integral in the observation group was (7.62 ± 4.08), which was lower than the (9.89 ± 4.71) in the control group, the difference was significant ( t = 2.14, P<0.05). After treatment of 3 days, the scores of quality of life in the five dimensions of dysthymia, behavior disorder, health worry, avoidance of eating and social function in the observation group were (82.44 ± 11.46), (80.25 ± 11.67), (76.23 ± 12.67), (59.80 ± 15.14) and (79.23 ± 11.59) points, which were different with the (73.57 ± 12.39), (72.35 ± 15.48), (69.76 ± 13.11), (50.00 ± 16.17) and (73.04 ± 13.11) points in the control group, the difference were significant ( t values were -3.09 - -2.08, all P<0.05). Three days after treatment, the score of IBS-SSS and Bristol fecal character in the observation group were (118.24 ± 40.64) and (5.09 ± 0.62) points, which were lower than the (146.86 ± 60.09) and (5.51 ± 0.66) points in the control group, the difference were significant ( t = 2.31 and 2.76, both P<0.05). After treatment, the score of HADS-A and HADS-D in the observation group were (6.26 ± 1.75) and (5.29 ± 1.47), which were different with the (7.26 ± 2.19) and (6.17 ± 2.11) in the control group, the difference were significant ( t = 2.08 and 2.00, both P<0.05). Conclusions:Shumu Peitu herb-partitioned moxibustion can effectively improve IBS-D patients with liver-stagnation and spleen-deficiency pattern, relieve clinical symptoms, reduce negative emotions, and improve quality of life.

The present study aims to explore the genetic diversity of germplasm resources of Chrysanthemum×morifolium (hereinafter, C.×morifolium) at the molecular level and to establish a fingerprint database of C.×morifolium varieties. We employed 12 pairs of primers with high levels of polymorphism, clear bands, and high degrees of reproducibility to analyze the SSR molecular markers and genetic diversity of 91 C.×morifolium materials and 14 chrysanthemum- related materials. With regard to constructing the fingerprints of the tested materials, we chose 9 pairs of core primers. The findings revealed that 12 primer pairs detected 104 alleles in 105 samples, ranging from 2 to 26. The average number of observed alleles (N_a) per site was 9.25. The average number of effective alleles (N_e) per site was 2.745 6, with its range being 1.276 0 to 4.742 5. Shannon genetic diversity index (I) values ranged between 0.513 3 and 2.239 9 (M=1.209 0). Nei's gene diversity index (H) ranged between 0.216 3 and 0.789 1 (M=0.578 0). The observed heterozygosity (H_o) ranged between 0.223 3 and 0.895 2 (M=0.557 5). The expected heterozygosity (H_e) ranged between 0.217 4 and 0.793 3 (M=0.580 8). The polymorphism information content (PIC) ranged between 0.211 5 and 0.774 0 (M=0.532 9). The genetic similarity (GS) ranged between 0.228 5 and 1.000 0 (M=0.608 3). Cluster analysis revealed that when the genetic distance (GD) equals to 0.30, the tested materials can be classified into 2 groups. When the GD equals to 0.27, the first group can be divided into 6 subgroups; accordingly, 105 tested materials can be divided into 7 subgroups. The cophenetic correlation test was carried out based on the cluster analysis, and the corresponding results showed that the cluster map correlated with the genetic similarity coefficient (r=0.952 73). According to the results of Structure population analysis, we obtained the optimal population number, with the true number of populations (K) being 3 and the population being divided concerning Q≥0.5. Three subgroups, i.e., Q1, Q2 and Q3, included 34, 33 and 28 germplasms, respectively, and the remaining 10 germplasms were identified as the mixed population. During the experiment, 9 pairs of core primers were screened among the total of 12 for a complete differentiation regarding 105 tested materials, and the fingerprints of 91 C.×morifolium materials and 14 chrysanthemum-related materials were further constructed. Overall, there were significant genetic differences and rich genetic diversity among C.×morifolium materials, which would shed light on the garden application and variety selection fields of C.×morifolium. The fingerprint database of 105 C.×morifolium varieties and chrysanthemum-related species may provide technical support for future research regarding the identification and screening system of C.×morifolium varieties.
Genetic Variation ; Chrysanthemum/genetics* ; Reproducibility of Results ; Microsatellite Repeats/genetics* ; Polymorphism, Genetic ; Biomarkers ; Phylogeny

Objective:To explore the factors associated with severe bleeding after percutaneous nephrolithotomy (PCNL) in male patients and evaluate the efficacy of interventional embolization.Methods:A retrospective case series study was conducted at Nankai Hospital of Tianjin, China, from January 2018 to October 2021. The clinical data of 230 male patients with upper urinary tract stones were analyzed. The observation indicators included age, hypertension, diabetes, renal function abnormalities, history of preoperative anticoagulant use, stone size, stone type, number of puncture channels, operation time and degree of hydronephrosis. To describe the clinical characteristics of bleeding after percutaneous nephrolithotomy in men, and analyze the factors associated with severe bleeding after PCNL. Single factor analysis was performed using the Chi-square ( χ2) test, and multivariate analysis was performed using logistic regression analysis. Results:Univariate analysis showed that diabetes mellitus ( χ2=4.90, P=0.027), abnormal renal function ( χ2=18.32, P<0.001), history of preoperative oral anticoagulants ( χ2=5.10, P=0.024), abnormal bleeding and coagulation function ( χ2=8.22, P=0.004) and the number of puncture channels ( χ2=22.08, P<0.001) were the related factors affecting bleeding after PCNL. Multivariate logistic regression analysis showed that diabetes mellitus ( P=0.032), abnormal renal function ( P<0.001), and the number of puncture channels ( P<0.001) were the independent risk factors of bleeding after PCNL. Of the 28 patients with bleeding after PCNL, 25 were treated with interventional embolization, with a technical success rate of 100.0% and a clinical success rate of 89.3%. Conclusions:For patients with renal calculi and comorbid diabetes, renal function abnormalities, and multiple punctures, relevant preventive measures should be actively administered before PCNL to reduce the risk of postoperative bleeding. For patients with severe bleeding of the kidney after PCNL, TAE is a safe and effective minimally invasive treatment method.

There are a large number of atypical symptoms and undifferentiated diseases in general practice, in which sociological factors are deeply involved. So to incorporate social factors into clinical thinking and to interpret disease from the perspective of the patient′s social life will enrich the multidisciplinary medical model and holistic care in general practice. This article shares author′s clinical experience through introducing typical cases, in which the patient′s social life and behavior characteristics played certain roles in disease development. The article also explores a new way from the sociological perspectives to interpret the complex symptoms that are difficult to be explained clinically by biomedicine or psychology.

Symptom cluster intervention is conducive to achieving the simultaneous improvement of multiple symptoms and improving the efficiency of symptom management. This paper aims at reviewing the application of symptom cluster management in traditional Chinese medicine (TCM) nursing, analyzing its rationality and feasibility, and further exploring the value of applying symptom cluster management to TCM nursing.

Objective:To explore the effects of toilet-type smart fumigator on patients with anal edema after anal surgery.Methods:To tally 70 patients with anal edema after anal surgery in the Department of Proctology at Nanjing Hospital of Chinese Medicine Affiliated to Nanjing University of Chinese Medicine between April and July 2019 were selected by convenient sampling, and randomly divided into an observation group and a control group, with 35 patients in each group. Patients in the observation group received Traditional Chinese Medicine (TCM) fumigation with a toilet-type smart fumigator, while patients in the control group received TCM fumigation with an ordinary bidet. After TCM fumigation, both groups were routinely treated with microwave. The efficacy, VAS score, perianal swelling score, and patient comfort score of the two groups were compared.Results:After intervention, the total effective rate of treatment in the observation group was 97.1% (34/35) , higher than that 74.3% (26/35) in the control group, and the difference between the two groups was statistically significant ( P<0.05) ; the VAS score of the observation group was (2.03±0.86) , lower than that (2.46±0.85) of the control group, and the difference between the two groups was statistically significant ( P<0.05) ; the perianal swelling score of the observation group was (0.29±0.46) , lower than that (0.57±0.50) in the control group, and the difference between the two groups was statistically significant ( P<0.05) ; the comfort score of the observation group was (1.94±0.54) , lower than that (2.26±0.56) in the control group, and the difference between the two groups was statistically significant ( P<0.05) . Conclusions:The effect of the toilet-type smart fumigator for TCM fumigation on patients with anal edema after anal surgery is better than the ordinary bidet, which can effectively relieve the patient's pain and edema, extend their comfort, and improve their prognosis.

Objective? To expLore the curricuLum setting of traditionaL Chinese medicine (TCM) nursing practice, and to provide a theoreticaL reference for TCM nursing training in communities and CLassⅡ and CLassⅢ hospitaLs. Methods? The entries of TCM nursing practice courses were deveLoped by Literature review, group discussion and DeLphi expert enquiry between January 2016 and June 2017. ResuLts? The coLLection rate of questionnaires during the two rounds of expert enquiry was 100% and 80%, respectiveLy. The authority coefficient (Cr) of the two rounds of expert enquiry was 0.86 and 0.84, respectiveLy. The KendaLL coefficient of concordance (W) during the two rounds of expert enquiry in terms of secondary indicators was 0.425 and 0.345, and the W in terms of tertiary indicators was 0.268 and 0.308, respectiveLy. The experts' opinions tended to converge in the two rounds of enquiry. FinaLLy, 1 primary indicator, 3 secondary indicators and 40 tertiary indicators were incLuded in the curricuLum setting. ConcLusions? This study outLines the content of TCM nursing practice courses for the training system of TCM nursing taLents, whose reLiabiLity and vaLidity is high and which may be a vaLuabLe reference.

Objective Based on the theory of "lung and large intestine form", to observe the improvement of gastrointestinal function and pulmonary symptoms in patients with acute exacerbation of chronic obstructive pulmonary disease with phlegm heat accumulation and find the best meridians and acupoints. Methods A total of 120 patients with acute exacerbation of chronic obstructive pulmonary disease with phlegm heat accumulation and gastrointestinal dysfunction were randomly divided into intervention 1 group (lung meridian group), intervention 2 group (large intestine meridian group) and intervention 3 group (lung meridian and large intestine meridian group) and control group. There were 30 cases in each group. The control group was given conventional treatment and nursing. The intervention group was treated with acupoint massage along meridians, 1 times a day for 20 minutes, and the intervention lasted for 10 days. The scores of gastrointestinal symptoms, clinical symptom scores and C reactive protein among 4 groups were compared. Results The scores of gastrointestinal symptoms, clinical symptom scores and C reactive protein were decreased in all groups after intervention compared with those before intervention, and the difference was statistically significant (F=9.736, 2.980, 0.894, P<0.01 or 0.05). After intervention, the difference of the scores of gastrointestinal symptoms among four groups were statistically significant (Ftime=213.423, F interaction =2.540, Fgroup =3.184, P<0.01 or 0.05). The difference of the scores of clinical symptoms among four groups were statistically significant( Ftime =337.859, P<0.01). The difference of C reactive protein among four groups was statistically significant (F=0.894, P<0.05). Conclusions Acupoint massage can effectively reduce the score of the symptoms of the gastrointestinal tract, the integral of clinical symptoms and the C reaction protein, and the effect of the massage of the meridians and collaterals. The effect of massage in the lung and the coliform meridian acupoint group is the best.

OBJECTIVE: To prepare Formononetin (FMN) inclusion compound liposome and evaluate its quality. METHODS: FMN inclusion compound liposome was prepared by film dispersion method. The morphology, particle size, Zeta potential, encapsulation efficiency and in vitro release properties were studied. RESULTS: The particle size, Zeta potential and encapsulation efficiency of prepared FMN inclusion compound liposome were (255. 34 ± 12. 87) nm, (25. 32 ± 3. 51) mV, (81. 63 ± 0. 79)％, respectively (n=3). The 24 h accumulative release rate of prepared FMN inclusion compound liposome was 56. 12％. CONCLUSIONS: FMN inclusion compound liposome with good sustained-release effect is prepared successfully and in line with related quality standard.

Objective To investigate the effect of acupuncture decompression operation on the quality of life of patients with mild to moderate essential hypertension. Methods 74 cases of mild to moderate essential hypertension were divided into experimental group and control group by random number table, 4 cases were lost. Finally, a total of 70 patients were included in the data analysis. In the control group, 35 cases were given routine drug taking and health guidance, the experimental group of 35 patients increase the treatment of acupuncture decompression operation points on this basis. The two groups of patients were measured with blood pressure before and after intervention, and World Health Organization Quality of Life (WHOQOL-BREF) was used to evaluate the quality of life, and the data were analyzed. Results The scores of physiological and psychological fields in the experimental group were (15.23 ± 2.66) points and (16.68 ± 2.23) points while the control group were (13.98 ± 2.18) points, (15.25 ± 2.52) points, the difference was statistically significant (t=2.305, 2.522, P<0.05). The experimental group of social relations, environmental score were respectively (1.37 ± 2.24) points, (14.16 ± 1.24) points, while the control group were (13.29 ± 2.30) points, (13.56 ± 1.36) points, 2 groups were not statistically significant (t=0.770, 3.063, P > 0.05). The experimental group systolic blood pressure, diastolic blood pressure were (121.43 ± 6.43) mmHg (1 mmHg=0.133 kPa), (71.74 ± 3.88) mmHg, compared with the control group were (128.34±5.72) mmHg, (76.06±4.43) mmHg. The difference was statistically significant (t=-4.753,-4.210, P<0.05). Conclusions acupuncture decompression operation can improve blood pressure in young and middle-aged patients with hypertension, improve the quality of life.