Objective? To investigate the prevalence of food addiction among newly diagnosed type 2 diabetes mellitus (T2DM) patients in Xinjiang region and to analyze its influencing factors. Methods? From January 2017 to December 2017, we selected 279 newly diagnosed T2DM patients in Endocrinology Outpatient Department of the First Affiliated Hospital of Xinjiang Medical University as subjects by convenience sampling. The Yale Food Addiction Scale was used to judge the food addiction of patients. All selected patients were divided into group of food addiction and group of non-food addiction based on results. We collected and compared the clinical data both groups, and used Spearman correlation to analyze the correlations between food addiction and clinical data, and used Logistic regression to analyze the influencing factors of food addiction. Results? A total of 39 patients were judged as food addiction with 13.98% (39/279) for the proportion. In group of food addiction, the body mass index (BMI), waistline, waistline/height, fasting blood glucose (FBG), 2-hour postprandial plasma glucose (2 h-PG), triacylglycerol and insulin resistance index were higher than those in group of non-food addiction, and the insulin secretion index was lower than that in control group,the differences were all statistically significant(P＜0.05). Spearman correlation analysis showed that food addiction had positive correlations with the sexes, BMI, waistline, waistline/height and FBG, and had a negative correlation with the ages (P＜0.05). Logistic regression analysis showed that the influencing factors of food addiction among newly diagnosed T2DM patients included sexes, ages and BMI (P＜ 0.05). Conclusions? The proportion of food addiction is high among newly diagnosed T2DM patients in Xinjiang region of China which is related to sexes, ages and BMI. Medical staff should pay attention to the screening of food addiction and intervention for life style so as to prevent diabetes and other metabolism diseases.

Objective To construct prokaryotic expression vectors for glutamate dehydrogenase(GDH)of Clostridium difficile(CD),and express recombinant GDH in Escherichia coli,and identify its antigenicityed.Methods The entire gene of GDH was cloned from ATCC43255 genome DNA.The recombinant antigens were expressed in E.coli with IPTG induction and purified by Ni-NATBeads.The antigenicity was detected using CD Qick Chek Complete dual-antigen EIA. Results Prokaryotic expression vectors of CD GDH were constructed successfully.The antigen could be identified by specific anti-GDH antibodies.Conclusion The GDH antigen can be used to prepare corresponding antibodies,which facilitate the development of immunoassay for CD GDH.