Objective: To explore the correlation between sleep and laryngopharyngeal reflux disease by epidemiological approaches. Methods: From May 1, 2017 to April 30, 2018, data of age, gender, height, weight, smoking, alcohol consumption, constipation and high fat diet in patients in Otorhinolaryngology specialist clinic, the Eighth Medical Center, General Hospital of the Chinese PLA were retrospectively analyzed. Reflux Symptom Index (RSI), Pittsburgh Sleep Quality Index (PSQI) and Hospital Anxiety and Depression Scale (HADS)were filled. According to RSI scores, patients were divided into case group and control group. The differences of the above indicators between the two groups were compared by Stata 12.0 software, and the risk factors of LPRD were analyzed by multivariate Logistic regression. Results: A total of 908 patients were enrolled, including 166 in the case group and 742 in the control group. There was no significant difference in BMI, smoking, drinking, constipation and high fat diet between the two groups (all P>0.05). The PSQI, anxiety and depression score of the case group were higher than those of the control group. The anxiety and depression scores of the patients with sleep disorders in the case group were significantly higher than those of the normal sleepers (all P<0.05). RSI of the patients with sleep disorders was higher than that of the patients with normal sleep(9.5[4.0,16.0]vs. 5.0[1.0,10.0], Z=-6.07, P<0.001). Multivariate analysis showed that sleep disorder was the risk factors of LPRD (OR=2.59, 95%CI 1.75-3.84). Conclusions Sleep disorder is related to the occurrence of LPRD. The association between LPRD and sleep disturbances is bidirectional. Sleep disorder may also be related to the anxiety and depression in LPRD patients. Handling sleep disorder timely may benefit LPRD patients.

Objective To investigate the changes of component and morphology in internal carotid vulnerable plaque,for helping to make clinical intervention strategy individually. Methods A total of 47 patients with internal carotid vulnerable plaques and primary hypertension underwent 2 high-resolution and multi-contrast MRI scans, from March 2008 to April 2014 were retrospectively reviewed. At baseline, the plaque was mainly located at the proximal internal carotid artery,and maximum plaque thickness ≥1.5 mm with intraplaque hemorrhage(IPH)and(or)thin or ruptured fibrous cap.Interscan interval was 0.5 years and above. Patients with carotid occlusion or surgery were excluded. Morphological measurements included maximum plaque thickness, maximum plaque area and cross-sectional vessel area (CSVA) on the level of plaque with maximum thickness. The paired-samples t test was performed to compare the difference of plaque morphology between baseline and follow-up carotid MRI.Results The interscan interval was 1.83 (1.59,1.99)years for 47 internal carotid vulnerable plaques.One case(interscan interval 2.16 years)showed IPH within those 11 plaques without IPH at baseline,and one case(interscan interval 1.42 years)had new incident IPH within those 36 plaques with IPH at baseline. Maximum plaque thickness increased significantly from(3.94±1.44)mm to(4.24±1.68)mm(t=2.30,P<0.05)by 5.14%(-3.83,11.34)% per year. Maximum plaque area increased significantly from(49.19±21.15)mm2to(56.03±24.91)mm2(t=3.87,P<0.01)by 6.67%(-2.26,19.60)% per year.CSVA increased significantly from(66.22±27.51)mm2to(73.68±31.47)mm2(t=4.08,P<0.01)by 5.18%(-1.63,12.34)% per year.Conclusion The progression of component,burden and outer remodeling in the internal carotid vulnerable plaque may be faster in hypertension, therefore reasonable intervention strategy and regular follow-up carotid MRI should be performed.

Purpose To investigate the expression of pFRK protein in non-small cell lung cancer (NSCLC) patients and to explore its prognostic value.Methods The expression of p-FRK protein in tumor tissues and corresponding adjacent normal pulmonary tissues from 162 NSCLC patients was detected by immunohistochemistry of EnVision two-step.The correlation among p-FRK expression,age,gender,clinicopathologic features,pTNM stage and metastasis of NSCLC patients was also analyzed.whether p-FRK could be used as an independent predictor of prognosis for patients with NSCLC was further determined.Results The positive expression rate of p-FRK in NSCLC tissues (51.9％) was significantly higher than that of adjacent normal lung tissues (11.7％) (P =0.001).The expression of p-FRK in NSCLC was significantly correlated with histopathologic type,lymph node status and pTNM stage (P ＜0.05).Kaplan-Meier survival analysis showed that there was a negative correlation between p-FRK positive rate and postoperative overall survival (x2 =17.849,P ＜0.001).Multivariate analysis showed that p-FRK expression was an independent prognostic factor for overall survival of NSCLC patients (HR =0.496,95％ CI:0.295-0.836,P ＜ 0.05).Conclusion As a novel NSCLC biomarker,the expression of p-FRK may predict a poor prognosis in the patients with NSCLC.

Objective To assess the non-invasive and quantitative value of diffusion tensor imaging (DTI)in the diagnosis,differ-ential diagnosis and classification of renal occupying lesions.Methods 30 healthy volunteers and 126 suspected cases with renal space-occupying lesions underwent conventional magnetic resonance imaging (MRI)and DTI scans.A total of 144 lesions were found in 126 patients.DTI was performed in 6 directions with b values of 0 and 500 s/mm2 .Fractional anisotropy (FA)and appar-ent diffusion coefficient(ADC)values were measured.Results With b values at 500 s/mm2 ,FA and ADC values between the nor-mal renal cortex and medulla in renal carcinoma,renal angiomyolipomas,renal cysts were pairwisely compared and there were all statistical difference (P <0.05).Difference of FA and ADC values between clear renal cell carcinoma and non-clear cell carcinoma was also significant (P <0.05).Conclusion FA and ADC values could distinguish benign from malignant renal tumour which may be helpful in the speculation of renal carcinoma pathological types.

Survivin,the smallest and structurally unique member of the inhibitor of apoptosis protein (IAP)family,is highly expressed during fetal development and rarely expressed in self -renewable healthy adult tissues.Moreover,it exists in most malignant tissues .Survivin is described as a bifunctional protein involving in the regulation of cell proliferation and apoptosis in normal and cancer cells .Due to its structural and functional distinctiveness ,survivin is currently attracting considerable attention as a new target for cancer molecular targeted therapy.Strategies to targeting Survivin include antisense oligonucletides ,ribozymes,siRNA,dominant negative mutants,small molecule antagonists and immunotherapy .

A new closed bipolar electrode electrochemiluminescence ( ECL)-based device was designed, and further used to investigate the ECL behaviors of luminol in this device. Our results showed that, while a suitable voltage was applied to the two poles of the closed bipolar electrode, both the positively charged ions and luminol-based anionic ions could be enriched on the two poles of the closed bipolar electrode, respectively. More importantly, the ECL signals, generated from the electro-oxidation of luminol on anodic pole, were found to be related to the total amount of positively charged ions on the cathodic pole of the closed bipolar electrode. Under the optimum experimental conditions, the ECL response was linearly to the concentration of analyte in the range of 1. 0×10-9-1. 0×10-8 mol/L with a detecting limit of 1. 1×10-10 mol/L. Based on this finding, a new ECL method for sensing the solution conductance was developed.

Objective To study the CT manifestations and diagnostic value of stromal tumor.Methods CT documentation of 25 stromal tumor cases confirmed by surgery and pathology were analyzed retrospectively,all cases underwent 64 row or 256 row spiral CT plain scan and enhanced scan.Results Within 25 cases,1 7 cases were gastrointestinal,of which,10 cases were found in stom-ach,1 case in duodenum,3 cases in jejunum and 3 cases in ileum;and 8 cases were extra-gastrointestinal,of which,6 cases were found in enterocoelia,1 cases in inguinal region,1 case in abdominal wall;the ratio was 2.1 ︰ 1.In 25 cases,highly malignant dan-ger level were 1 1 cases,7 cases of moderate and 7 cases of low malignant danger;Transfer occurred in 7 cases and 3 cases of postop-erative recurrence.Conclusion The volume CT has great advantages in displaying information in lesion,and has high value in loca-ting the lesion and qualitative diagnosis of disease.

Objective To analyze the imaging features of mycobacterium in AIDSpatients.Methods Twenty-three cases of mycobacterium tuberculosis and 13 patients of non-tuberculous mycobacteria were proved etiologically and included in this study.All patients underwent X-ray and CT examinations,imaging data were analyzed and compared.Results The imaging findings of mycobacterium tuberculosis in AIDS patients included consolidation (n =11),pleural effusion (n =11),mediastinal lymphadenopathy (n =11).Pulmonary lesions were always diffuse distribution,and 14 patients of extrapulmonary tuberculosis were found.Pulmonary lesions in non-tuberculous mycobacteria tend to be circumscribed.Conclusions Non-tuberculous mycobacterial infection in AIDS patients is more common and usually combined with other infections.Imaging features are atypical.

Objective To study the procedure of imaging examination for the patients with colon cancer.Methods The comparative study on ultrosonograph, pneumobarium double contrast examination, colonscopy and CT in 82 cases with colon carcinoma comfired by operation and pathalogy were carried out.Results The detective rate of colon cancer was 90.1%,89.5%,53.8% and 53.3% by pneumobarium double contrast examination,colonscopy ultrosonography and CT respectively.The detective rate of metastasis in liver was 100% by ultrosonograph and CT.Conclusion Pneumobarium double contrast examination and colonoscopy are the main methods for diagnosis of colon cancer. Ultrosonography and CT are the important methods in evaluating the stage of colon carcinoma before operation.