Objective To summarize the nursing experience of non-invasive high- frequency ventilation in very low birth weight infants. Methods A total of 21 cases in 2017 were retrospectively analyzed. Ensuring effective ventilation, keeping the infants quiet, taking care of complication and strict observation were the key points. Results Sixteen cases had satisfied results, 2 cases were transferred to another hospital, and 3 cases died. Fourteen of the 21-cases successfully withdrawn from the machine, while 33.3％(7/21) of the cases were using invasive respiratory support. Conclusions Careful and meticulous nursing of non-invasive high-frequency ventilation can ensure the effectiveness of the invasive respiratory support, and promote the successful withdrawal, and reduce the intubation rate in very low birth weight infants.