In order to investigate the changes in lipid metabolism of the host after infection with porcine epidemic diarrhea virus(PEDV),blood,intestinal and cellular samples were collected from PEDV-infected piglets and Vero cells,and the levels of triglyceride(TG)and total cholesterol(TC)were detected by blood biochemical analyses,flow cytometry,and immunofluorescence,and changes in the gene levels of the enzymes related to lipid metabolism of the hosts were detected by qPCR.The results showed that PEDV infection significantly elevated host total lipid levels and sig-nificantly promoted the expression of enzymes related to fatty acid and cholesterol metabolism in the intestine and Vero cells of piglets.In conclusion,PEDV infection leads to an increase in host to-tal lipid levels,remodeling of lipid metabolism in piglets intestine and Vero cells,and lipid metabo-lism gene changes.The results of this study show that lipid metabolism-related genes can be used as a key target to influence PEDV replication,which will provide a new theoretical basis for the subsequent prevention,control and treatment of PEDV infection.