Objective To explore the intervention effect of the combination of medicine and education on teachers and parents of children with attention deficit hyperactivity disorder (ADHD).Methods The students in Grade 1 to 3 of 6 public primary school (6～11 years old) were enrolled by stratified sampling method and randomly divided into the experimental group and the control group.All the students were screened for ADHD,and the children diagnosed with ADHD and their parents and teachers were enrolled into study as the research object.The children in the control group were given oral short-effect methylphenidate hydrochloride tablets and their parents underwent routine health education,while the parents and teachers in the experimental group were given both the routine health education and systematic training.Before and after training,the awareness rates of ADHD knowledge among parents and teachers were observed,and the clinical effects of two groups were compared.Results The control group included 71 children with ADHD,71 pairs of parents and 31 teachers,while the experimental group included 68 children with ADHD,68 pairs of parents and 34 teachers.The difference of general data between the two groups has no statistical significance (P＞0.05).Before intervention,there was no significant difference in awareness rate of ADHD knowledge among teachers and parents in the two groups (P＞0.05).After the intervention,the awareness rate of ADHD knowledge in teachers of the control group did not change obviously,while which in parents of the control group and the parents and teachers in the experimental group were significantly improved,and the improvement effect in the experimental group was significantly better than that of the control group (P＜0.05).The scores of the SNAP-Ⅳ scale in both groups were significantly decreased,and the scores of the experimental group were significantly lower than those in the control group.The significant rate and total effective rates of the experimental group were significantly higher than those in the control group (P＜0.05).Conclusion The intervention effect of combination of medicine and education on teachers and parents of children with ADHD is definite,which is worthy of further research.

Basic medicine teaching is an important part of the medical student cultivation.Its contents are boring and complicated,and difficult to learn and remember.In the process of basic medicine teaching,educational researchers have been trying to combine a variety of teaching methods and apply them flexibly.They want to be able to fully mobilize the enthusiasm of the students.However,it is difficult to obtain a satisfactory result because of the limited number of course hours.The micro-media are open,interactive and no time limit.Therefore,combining micro-media with a variety of teaching methods in the teaching processis expected to create a new teaching mode of basic medicine and achieve a good teaching result.

Objective To detect the gene expression profiles of miRNA in joint fluids of patients with rheumatoid arthritis(RA)and healthy people and clinical significance.Methods The expressions of miRNAs in joint fluids of 3 RA patients and 3 healthy adults were detected by miRNA chip technology,and miRNAs with significant differential expressions were selected to be validated by the qRT-PCR.Correlations between selected miRNA and clinical and laboratory indexes of RA were analyzed.Results In the gene expression profile of miRNA in joint fluids of patients with RA,the expressions of 23 miRNAs significantly changed when compared with those in the healthy people.The results of selected miRNAs by qRT-PCR were correspond with the Results of the miRNA chip technology.The difference of miR-146a expression in joint fluid between RA patients and healthy controls was the most significant.Pearson correlation analysis showed that serum miR-146a expression in joint fluid of RA patients was positively correlated with rheumatoid factor(RF)value and DAS28 score.ROC curve analysis showed that the area under the curve(AUC)of miR-146a was 0.71,and the sensitivity and the specificity of miR-146a in joint fluid in predicting RA were 85.29％and 61.54％respectively.Conclusion There is significant difference in expression of miRNAs in joint fluid between RA patients and healthy people.Expression of miR-146a increases significantly in joint fluid of RA patients,and it level is significantly correlated with the clinical and laboratory indexes of RA.

Objective To detect the gene expression profiles of miRNA in joint fluids of patients with rheumatoid arthritis(RA)and healthy people and clinical significance.Methods The expressions of miRNAs in joint fluids of 3 RA patients and 3 healthy adults were detected by miRNA chip technology,and miRNAs with significant differential expressions were selected to be validated by the qRT-PCR.Correlations between selected miRNA and clinical and laboratory indexes of RA were analyzed.Results In the gene expression profile of miRNA in joint fluids of patients with RA,the expressions of 23 miRNAs significantly changed when compared with those in the healthy people.The results of selected miRNAs by qRT-PCR were correspond with the Results of the miRNA chip technology.The difference of miR-146a expression in joint fluid between RA patients and healthy controls was the most significant.Pearson correlation analysis showed that serum miR-146a expression in joint fluid of RA patients was positively correlated with rheumatoid factor(RF)value and DAS28 score.ROC curve analysis showed that the area under the curve(AUC)of miR-146a was 0.71,and the sensitivity and the specificity of miR-146a in joint fluid in predicting RA were 85.29％and 61.54％respectively.Conclusion There is significant difference in expression of miRNAs in joint fluid between RA patients and healthy people.Expression of miR-146a increases significantly in joint fluid of RA patients,and it level is significantly correlated with the clinical and laboratory indexes of RA.

Cationic lipid nanoparticles possessing favorable biocompatibility and controllable particle size has at-tracted increasing attention in the field of non-viral vectors for delivering nucleic acids in vitro and in vivo.1,2-Dilinoleyloxy-3-dimethylaminopropane(DLinDMA)and its derivatives,one of the most important cationic lipid materials,have been widely studied as the mainly composition of cationic lipid nanoparticels.This paper summa-rized the structure,characteristics and clinical application of DLinDMA nanoparticles.

This paper points out the experimental animal ethics education in pharmacology teaching and the im-portance of the experiment,it is good to raises the student good humanity accomplishment and scientific research quality, and promote medical research and life ethics of benign interaction.It also expounds the basic content of ex-perimental animal ethics education,including:animal welfare, the 3R principle, AAALAC accreditation and analy-sis of the experimental animal welfare legislation status, raises questions about animal ethics education problems and thinking in the pharmacology experiment teaching in ourschool.The experimental animal ethics education should become an important part of pharmacology experimental teaching, which enhanced the students′s ethical awareness to better understand and respect for life, and contribute to the sustainable development of medical and pharmaceutical research.

Objective To evaluate the prevalence and potential risk factors for pulmonary hypertension in the elderly hospitalized patients. Methods1093 elderly hospitalized patients aged (80.8 ± 9.8) years were examined by Doppler echocardiography for evaluating pulmonary hypertension which defined by pulmonary artery systolic pressure (PASP)≥40 mm Hg (1 mm Hg=0.133 kPa).The relative clinical testing data were collected. Results 115 patients (10.5％) of the elderly patients were diagnosed as pulmonary hypertension.The prevalence of pulmonary hypertension in patients aged 85-100 years was 16.4％ (82/500),which was higher than in other ages. In the multivariate logistic regression analysis,age (OR=1.085,95％CI=1.025-1.148,P=0.005),left ventricular diastolic dysfunction (OR =3.954,95 ％ CI =1.812-8.627,P=0.001 ),internal diameter of right ventricle (OR=1.271,95％CI=1.112-1.452,P=0.000) and internal diameter of left atrium (OR=1.100,95％CI=1.013-1.194,P=0.023) were risk factors for pulmonary hypertension.ConclusionsThe prevalence of pulmonary hypertension is high in the elderly hospitalized patients,especially those aged 85 years and over,complicated with left ventricular diastolic dysfunction and enlargement of right ventricle and left atrium.

OBJECTIVE: To investigate the surgical method of inferomedial orbital decompression under nasal endoscope for malignant hyperthyroid exophthalmos and surgical effect. METHOD: Twenty one cases (27 eyes) of malignant hyperthyroid exophthalmos with poor visual function and appearance received part of inferomedial orbital wall excision. RESULT: The patients were followed up for 6 to 12 months after surgery. Ocular protrusion recessed 2-6 mm and averaged 4.85 mm in all cases. Visual acuity increased in 17 eyes, remained the same in 9 eyes and decreased in 1 eye. Diplopia appeared in 5 cases after surgery and disappeared in 3 months. CONCLUSION Part of inferomedial orbital wall excision under nasal endoscope has the advantage of clear visual field, accurate excision, little hurt and complications. It is an effective method for malignant hyperthyroid exophthalmos.
Adult ; Endoscopy ; Female ; Graves Disease ; surgery ; Humans ; Male ; Middle Aged ; Nose ; surgery ; Orbit ; surgery ; Treatment Outcome ; Young Adult

Objective To explore the effect of long-term enhanced external counterpulsation(EECP)on endothelium-dependent and endothelium-independent vasorelaxation in the carotid arteries of atherosclerotic piss. Method Totally 18 20-day-old male infant pigs were randomly divided into 3 groups according to feeding given: the normal[control group(n=6),the hypercholesterolemic control group(n=6)and the hypereholesterolemic +EECP group(n=6).Porcine model of hypercholesterolemia was made by feeding high-cholesterol diet.After EECP for 36 hours in the hypercholesterolemic+EECP group(n=6),carotid arterial rings were harvested from all animals and their vaso-relaxation response to different dose of Acetylchofine(Ach)and Sodium nitroprusside (SNP)were detected,respectively.Results As the dose of Ach varying between 10-8 mol/L and 10-5mol/L, endothelium-dependent vasorelaxation ratio of hypereholesterolemic piss with or without EECP treatment was significantly lower than that of the normal control group(P＜0.05),however,endothehum-dependent vasorelax- ation ratio in pigs with EECP treatment was obviously higher compared with hypereholesterolemic pigs without EECP treatment(P＜0.05)as the Ach ranged from 10-7 mol/L to 10-5mol/L.Similarly,as the concentration of SNP ranged fiun 10-8 mol/L to 10-5 mol/L.endothelium-independent vasorelaxafion ratio of both the hypercholesterolemic control group and the hypercholesterolemic+EECP group were significantly lower than that of the normal control group(P＜0.05),and end othelium-independent vasorelaxation ratio of the hypercholesterolemic+EECP group was significantly higher than that of the hypercholesterolemic control group (P＜0.05).Condusions Long-term EECP improves the impaired endothelium-dependent and endothelium independent vasorelaxalion function resulting from atherosclerosis.

OBJECTIVE:To screen formulation of Insulin cream and to investigate the effects of insulin cream on wound healing in rabbits.METHODS:The best formulation of insulin cream was selected from 8 kinds of formulations with appearance and stability as index.Rabbit trauma model were induced and divided into matrix group,blank group,Insulin cream groups(high-dose,medium-dose and low-dose).Wound healing rate and healing time were compared among those groups.RESULTS:The fifth formulation possessed good appearance and stability,which included albolene,cetanol,glycerin monostearate,glycerol,etc.Compared with matrix group,the wound healing rates were obviously increased at different time points in Insulin cream groups(P