Objective:To investigate the effect of motion correction (MC) on the calculated values of myocardial blood flow (MBF) and myocardial flow reserve (MFR) based on cadmium-zinc-telluride SPECT (CZT SPECT) images.Methods:Twenty-eight consecutive patients (10 males, 18 females, age: (60.75±11.62) years) with suspected or known coronary artery disease who underwent myocardial perfusion imaging (MPI) with dynamic CZT SPECT between June 2019 and August 2019 in TEDA International Cardiovascular Hospital were retrospectively analyzed. The MBF and MFR during rest imaging and stress imaging were quantitatively analyzed. Corridor 4DM software was used to calculate the stress MBF (sMBF) and MFR of the coronary artery branches and left ventricular (LV) before and after MC. The paired t test and Pearson correlation were used for data analysis. Results:The sMBF and MFR of LV before MC were (0.82±0.49) ml·min -1·g -1 and 1.69±0.68 respectively. After MC the two parameters increased to (1.05±0.64) ml·min -1·g -1 and 2.12±0.77 respectively ( t values: -4.87, -6.01, both P<0.001). The sMBF and MFR in left anterior descending (LAD), left circumflex (LCX), right coronary artery (RCA) and LV before MC were correlated with those after MC ( r values: 0.69-0.96, all P<0.001). If MFR <2.0 was used as the reference of impaired MFR, data before MC showed 19 patients (67.9%, 19/28) had impaired MFR, while 13 patients (46.4%, 13/28) had impaired MFR based on MFR values after MC. Conclusion:For MPI quantitative imaging with CZT SPECT, the calculated values of sMBF and MFR after MC is higher than those before MC, suggesting that MC is helpful to reduce the false positive results which may be caused by the " creep" effect of the heart.

Carrier-free multi-component self-assembled nano-systems have attracted widespread attention owing to their easy preparation, high drug-loading efficiency, and excellent therapeutic efficacy. Herein, MnAs-ICG nanospike was generated by self-assembly of indocyanine green (ICG), manganese ions (Mn²⁺), and arsenate (AsO₄ ^3-) based on electrostatic and coordination interactions, effectively integrating the bimodal imaging ability of magnetic resonance imaging (MRI) and fluorescence (FL) imaging-guided synergistic therapy of photothermal/chemo/chemodynamic therapy within an "all-in-one" theranostic nano-platform. The as-prepared MnAs-ICG nanospike had a uniform size, well-defined nanospike morphology, and impressive loading capacities. The MnAs-ICG nanospike exhibited sensitive responsiveness to the acidic tumor microenvironment with morphological transformation and dimensional variability, enabling deep penetration into tumor tissue and on-demand release of functional therapeutic components. In vitro and in vivo results revealed that MnAs-ICG nanospike showed synergistic tumor-killing effect, prolonged blood circulation and increased tumor accumulation compared to their individual components, effectively resulting in synergistic therapy of photothermal/chemo/chemodynamic therapy with excellent anti-tumor effect. Taken together, this new strategy might hold great promise for rationally engineering multifunctional theranostic nano-platforms for breast cancer treatment.

Objective To explore the efficacy and safety of traditional Chinese medicine(TCM) combined with chemotherapy in the prevention and treatment of postoperative recurrence and metastasis of locally advanced gastric cancer (LAGC) by meta-analysis. Moreover, we evaluated the efficacy of TCM on the quality of life, immune indexes, and toxic and side effects during adjuvant chemotherapy. Methods The CNKI, Wanfang, PubMed, and other databases were searched by computer. Randomized controlled trials (RCTs) were searched. After literature screening and data extraction, Review Manager 5.3 software provided by Cochrane was used for meta-analysis. Results A total of 18 RCTs were included. Compared with chemotherapy alone, TCM combined with chemotherapy could improve the KPS score and CD3⁺ and CD4⁺/CD8⁺ index levels. The incidence rates of postoperative leucopenia, hemoglobin reduction, thrombocytopenia, nausea and vomiting, diarrhea, and neurotoxicity were reduced. In terms of postoperative QLQ-C30 score, abnormal liver function, and abnormal renal function, the incidence of TCM combined chemotherapy was similar to that of chemotherapy alone, with no statistical difference. Compared with chemotherapy alone, TCM combined chemotherapy could reduce the 1-, 2-, 3-, and 5-year cumulative recurrence and metastasis rates and prolong the disease-free survival time. Conclusion Compared with chemotherapy alone in adjuvant chemotherapy, TCM combined chemotherapy could improve the immune level and KPS score of LACC patients after surgery, reduce the incidence of adverse reactions, as well as reduce the recurrence and metastasis rate of LAGC after surgery and DFS could be improved.

The chemotherapy combined with photothermal therapy has been a favorable approach for the treatment of breast cancer. In present study, nanoparticles with the characteristics of photothermal/matrix metalloproteinase-2 (MMP-2) dual-responsive, tumor targeting, and size-variability were designed for enhancing the antitumor efficacy and achieving "on-demand" drug release markedly. Based on the thermal sensitivity of gelatin, we designed a size-variable gelatin nanoparticle (GNP) to encapsulate indocyanine green (ICG) and doxorubicin (DOX). Under an 808 nm laser irradiation, GNP-DOX/ICG responded photothermally and swelled in size from 71.58 ± 4.28 to 160.80 ± 9.51 nm, which was beneficial for particle retention in the tumor sites and release of the loaded therapeutics. Additionally, GNP-DOX/ICG showed a size reduction of the particles to 33.24 ± 4.11 nm and further improved drug release with the degradation of overexpressed MMP-2 in tumor. In the subsequently performed

Recent studies have shown that programmed cell death 4 (PDCD4) modulates distinct signal transduction pathways in different pathological conditions. Despite acute and chronic immune responses elicited by ischemia contributing to the functional deterioration of the kidney, the contributions and mechanisms of PDCD4 in acute kidney injury (AKI) have remained unclear. Using two murine AKI models including renal ischemia/reperfusion injury (IRI) and cisplatin-induced AKI, we found that PDCD4 deficiency markedly ameliorated renal dysfunction and inflammatory responses in AKI mice. Consistently, upregulation of PDCD4 was also confirmed in the kidneys from patients with biopsy confirmed acute tubular necrosis from a retrospective cohort study. Moreover, we found that overexpression of

Objective:To investigate the clinical features of children’s acute facial trauma, and to explore the clinical effects of plastic surgery in the repair of children’s acute facial trauma.Methods:The clinical data of children with acute facial trauma who were admitted to Children’s Hospital Affiliated to Zhejiang University School of Medicine from June 2017 to June 2018 were retrospectively analyzed. All children received plastic surgery treatment, and were followed up at 3, 6 and 12 months after operation. Scar cosmesis assessment and rating (SCAR) was used to evaluate the scar condition, and the parents’ satisfaction with scar was investigated. The clinical characteristics of acute facial trauma in children were analyzed. Graphpad prism 8.0.0 was used for statistical analysis, one-way ANOVA was used for comparison between groups, LSD- t test was used for pairwise comparison between groups, multivariate regression analysis was used to explore the main factors affecting parents’satisfaction with scar. P<0.05 showed that the difference was statistically significant. Results:A total of 338 children (364 wounds) with acute facial trauma were included, consisting of 223 males and 115 females, aged from 1 to 14 years, with an average of 4.5 years. All wounds were successfully repaired by plastic surgery. The SCAR scores at 3 months, 6 months and 12 months after operation were 2.9±1.0, 1.9±0.7 and 1.8±0.8 respectively, showing significant difference among there groups( P< 0.01). The SCAR score at 3 months after operation was significantly different from that at 6 and 12 months after operation ( P<0.01). There was no significant difference in SCAR score between 6 months and 12 months after operation ( P>0.05). The parents’ satisfaction scores at 3, 6 and 12 months after operation were 7.4±0.7, 7.9±0.7 and 8.2±0.8 respectively, showing significant difference among there groups( P<0.01). The parents’ satisfaction score at 12 months after operation was significantly different from that at 3 and 6 months after operation ( P<0.01). There was no significant difference in parents’satisfaction score between 3 months and 6 months after operation ( P>0.05). Multivariate regression analysis showed that whether there were multiple wound affected areas and scar width were the main factors influencing patients’ satisfaction with scar. Conclusions:Acute facial trauma in children is mostly caused by blunt trauma, and it is common in the forehead. According to the characteristics of the wound, using plastic surgery technique to repair children’s acute facial trauma can achieve the best wound healing and improve the prognosis of scar. It is expected to further improve the patient’satisfaction.

Objective:To investigate the clinical features of children’s acute facial trauma, and to explore the clinical effects of plastic surgery in the repair of children’s acute facial trauma.Methods:The clinical data of children with acute facial trauma who were admitted to Children’s Hospital Affiliated to Zhejiang University School of Medicine from June 2017 to June 2018 were retrospectively analyzed. All children received plastic surgery treatment, and were followed up at 3, 6 and 12 months after operation. Scar cosmesis assessment and rating (SCAR) was used to evaluate the scar condition, and the parents’ satisfaction with scar was investigated. The clinical characteristics of acute facial trauma in children were analyzed. Graphpad prism 8.0.0 was used for statistical analysis, one-way ANOVA was used for comparison between groups, LSD- t test was used for pairwise comparison between groups, multivariate regression analysis was used to explore the main factors affecting parents’satisfaction with scar. P<0.05 showed that the difference was statistically significant. Results:A total of 338 children (364 wounds) with acute facial trauma were included, consisting of 223 males and 115 females, aged from 1 to 14 years, with an average of 4.5 years. All wounds were successfully repaired by plastic surgery. The SCAR scores at 3 months, 6 months and 12 months after operation were 2.9±1.0, 1.9±0.7 and 1.8±0.8 respectively, showing significant difference among there groups( P< 0.01). The SCAR score at 3 months after operation was significantly different from that at 6 and 12 months after operation ( P<0.01). There was no significant difference in SCAR score between 6 months and 12 months after operation ( P>0.05). The parents’ satisfaction scores at 3, 6 and 12 months after operation were 7.4±0.7, 7.9±0.7 and 8.2±0.8 respectively, showing significant difference among there groups( P<0.01). The parents’ satisfaction score at 12 months after operation was significantly different from that at 3 and 6 months after operation ( P<0.01). There was no significant difference in parents’satisfaction score between 3 months and 6 months after operation ( P>0.05). Multivariate regression analysis showed that whether there were multiple wound affected areas and scar width were the main factors influencing patients’ satisfaction with scar. Conclusions:Acute facial trauma in children is mostly caused by blunt trauma, and it is common in the forehead. According to the characteristics of the wound, using plastic surgery technique to repair children’s acute facial trauma can achieve the best wound healing and improve the prognosis of scar. It is expected to further improve the patient’satisfaction.

OBJECTIVE: To improve the method of vermillion flap and orbicularis oris bundle anastomosis in repair of transverse facial cleft. METHODS: Based on the precise fixed point, the modified vermillion flap was designed slender at the new corner of the upper lip, and was inserted into the lower lip after removing part tissue. The orbicularis oris was divided into two bands and cross-stitched. RESULTS: Fifteen patients with unilateral transverse facial cleft form the Children's Hospital of Zhejiang University during September 2016 and December 2018 were operated, and the position and shape of the commissure were almost normal. CONCLUSIONS The cosmetic effect and oral function are satisfactory when the modified vermillion flap and bundle anastomosis of orbicularis oris is used to repair transverse facial cleft.
Anastomosis, Surgical ; Child ; Cleft Lip ; surgery ; Facial Muscles ; surgery ; Humans ; Lip ; surgery ; Surgical Flaps ; surgery ; Treatment Outcome

Objective: To investigate the expressions of programmed death ligand 1（PD-L1）in triple-negative breast cancer (TNBC) and its correlation with angiogenesis. Methods: 120 cases of TNBC patients who underwent surgery in the Fourth Hospital of Hebei Medical University from March 1, 2011 to June 1, 2012 were collected. The tumor tissues of patients were surgically resected and confirmed by pathology. PD-L1 protein expression in TNBC tissues of 120 patients was detected by tissue microarray combined with immunohistochemistry, and its relationship with various clinical indicators was analyzed. Blood vessels and lymphatic vessels were labeled withCD34andD2-40todetectmicrovesseldensity(MVD)andlymphaticvesseldensity(LVD)inTNBC.Results:Thepositiveexpression rate of PD-L1 in the tumor cells and interstitial infiltrating lymphocytes fromTNBC was 56.7% (68/120); No correlation was found between PD-L1 protein expression and the gender, age, histological grade, clinical stage, or tumor size of patients with TNBC (P>0.05), but related to the lymph node metastasis (P<0.05) and vascular thrombus (P<0.05). TNBC with high PD-L1 expression exhibited high incidence of lymph node metastasis and formation of vascular thrombus, and the expression of PD-L1 was positively correlated with MVD (r=0.500, P=0.02) as well as LVD (r=0.662, P=0.01). Log-Rank test showed that the survival time of TNBC patients with positive PD-L1 protein expression was significantly shorter than that of patients with negative expression (P<0.05). Cox multivariate analysis suggested that PD-L1 protein expression could be an independent prognostic factor for TNBC overall survival. Conclusion: PD-L1 plays an important role in TNBC angiogenesis and lymphangiogenesis, and is closely related to TNBC invasion and metastasis; blocking PD1/PD-L1 signal pathway is expected to be an effective new strategy for TNBC treatment.

Objective To construct a collagen matrix-based skin model that can last a long time in vitro for potential application of subsequent tests and studies.Methods The porcine collagen and normal human skin fibroblasts were mixed and seeded into the culture dish,which were cultured for 3-4 days to obtain a dermal structure.Then,the third-to seventh-passage normal human keratinocytes were seeded onto the dermal surface and cultured for 14 days to obtain a double-layer skin model.Hematoxylin and eosin (HE)staining and Masson staining were performed to evaluate the morphology and structure of the skin model,and electron microscopy was conducted to observe the ultrastructure of the skin model.Immunohistochemical study and immunofluorescence staining were conducted to determine the expression of major markers in each layer of the skin model.Results HE and Masson staining showed that the skin model and normal human skin tissues showed very similar dermal and epidermal structures.After harvest of the skin model,it can be cultured in vitro for another 14 days with favorable and stable dermal and epidermal structures.Electron microscopy showed lipids in the stratum corneum,keratohyalin granules in the stratum granulosum,corneodesmosomes,desmosomes and basal membrane in the skin model.Immunohistochemical and immunofluorescence staining showed the consistent expression of transglutaminase,filaggrin,keratin 10 and Ki67 in the epidermis in both the skin model and normal skin.Moreover,the expression of type Ⅳ collagen and laminin-5 in the basal membrane,as well as that of type Ⅰ collagen,type Ⅲ collagen and fibrillin in the dermis were both consistent between the skin model and normal skin.Conclusion The constructed three-dimensional (3D) skin model is highly analogous to normal human skin in the aspect of tissue structure and various protein expression,and the skin model can be stably cultured in vitro for at least 14 days after harvest.