Objective:To investigate the clinicopathological features, immunophenotype, diagnosis and differential diagnosis of mammary analogue secretary carcinoma of salivary gland (MASC) .Methods:From Jan. 2018 to Jan. 2021, 28 cases of salivary gland MASC were collected in Ningbo Diagnostic Pathology Center, and 10 cases of acinar cell carcinoma and 10 cases of adenoid cystic carcinoma were selected as controls. Immunohistochemical envision method was used to detect the expression of S-100,, DOG-1, CD117, SOX-10, Mammaglobin and Vimentin, and fluorescence in situ hybridization was used to detect the fusion gene of ETV6-NTRK3.Results:The S-100 protein, SOX-10 and Vimentin of MASC of salivary gland were diffusingly positive (28/28) , Mammaglobin (22/28) and CD117 (19/28) were partially positive, and DOG-1 was negative. ETV6-NTRK3 fusion transcription was successfully detected in 26 of 28 salivary gland MASC cases, of which 23 were positive and 3 were negative.Conclusions:Salivary gland MASC is a low-grade malignant epithelium tumor. Comprehensive detection of the expression levels of S-100 protein, SOX-10, DOG-1, Mammaglobin and CD117 is of great value for the diagnosis and differential diagnosis of MASC. FISH detection of ETV6-NTRK3 gene fusion has important reference value for definite diagnosis.

Objective: To assess the performance of extracorporeal membrane oxygenation (ECMO) in the treatment of neonates with critical diseases. Methods: This study retrospectively analyzed the clinical data of nine critically ill neonates treated with ECMO because of cardiopulmonary failure due to respiratory disorders in Children's Hospital of Fudan University from August 2015 to April 2018. General information, diagnosis, indications and approaches of ECMO, clinical procedure of ECMO, laboratory results, weaning time, survival rate before discharge, length of stay, and mechanical and neurological complications were collected and described. Results: (1) There were six male and three female patients with an average gestational age and body weight of 39.6 weeks (35-41 weeks) and 3 600 g (2 580-4 650 g), respectively. Out of them, six cases survived after successfully weaning from ECMO. (2) Sepsis (two cases), meconium aspiration (two cases), pulmonary hypertension (three cases), diaphragmatic hernia (one case) and pulmonary dysplasia (one case) were diagnosed in the patients. All underwent veno-arterial (VA) ECMO using centrifugal pump. The median age to initiate ECMO was 40 h (23-100 h) after birth, and the median duration of ECMO support was 240 h (70-370 h). During the treatment with ECMO, cholestasis, intracranial hemorrhage and vocal cord paralysis occurred in three cases, and mechanical complications (mainly were bleeding, hemolysis, oxygenated membrane leakage and embolization) occurred in five cases. The mean length of hospital stay for the six survivors was 24 d (20-49 d), and two of them developed neurological complications mainly manifested as cerebral infarction. There were three died cases. One was a baby with diaphragmatic hernia who received hernia repair during ECMO after which celiac space syndrome and necrotizing enterocolitis were developed and his parents refused further treatment. In the second case, the parents gave up treatment when no improvement was achieved after two weeks of ECMO support. While the last case was complicated by severe cerebral hemorrhage during ECMO and died after receiving no further treatment. Conclusions ECMO is newly applied in the treatment of neonates in China and of great significance for critically ill neonates. However, much need to be learned about its utilization in this population considering the mortality and disability rate are still high.

Objective To investigate the clinicopathological features and prognoses of papillary glioneuronal tumor (PGNT), and to improve the understanding of clinical worker on the tumor. Methods Three patients with PGNT got conformation in our center from July 1, 2011 to Apral 20, 2019, were chosen. The clinical features, histopathological characteristics, immunohistochemical findings, and prognoses of these patients were retrospectively analyzed. Results Two patients were female and one was male. Two tumors were located in the lateral ventricle and one in the temporal lobe. All patients presented with headache, and two exhibited dizziness. Imaging showed solid or cystic mass with well-defined boundary. Microscopically, it was characterized by papillary structures covered by single to multiple layers of small cuboidal gliocytes, and neurocyte-like cells scattered between the papilla. Immunohistochemically, the tumor cells covering papilla were positive for glial fibrillary acidic protein and oligodendrocyte transcription factor 2, and neurocyte-like cells were positive for synaptophysin; and the tumor cells were negative for isocitrate dehydrogenase 1 and silk/threonine specific protein kinase gene BRAF V600E. The proliferating cell nuclear antigen Ki-67 labeling index ranged from 1％-2％. Three patients were followed up for 12, 24 and 48 months after tumor resection, respectively, and no recurrence was found. Conclusion PGNT is an uncommon and variant of mixed neuronal-glial neoplasm with low-grade malignancy; the prognosis is good after gross total resection.

It has been suggested that HCN1 is primarily expressed in hippocampus, however little is known about its effects on spatial learning and memory. In the present study, we investigated the effects of non-specific HCN1 blocker CsCl on spatial learning and memory by using Morris water maze and in situ hybridization in mice. The results showed CsCl 160 mg/kg ip for 4 days, and the mean escape latency was 34 s longer than that of normal control (P<0.01). In hippocampal tissues, staining for the HCN1 mRNA was stronger in the DG and CA1 region of the hippocampus (P <0.05, P<0.05, when CsCl-administration group was compared with normal group). Our results suggested that CsCl could significantly affect the spatial learning and memory in mice, and HCN channel is involved in the process of learning and memory.

It has been suggested that HCN1 is primarily expressed in hippocampus, however little is known about its effects on spatial learning and memory. In the present study, we investigated the effects of non-specific HCN1 blocker CsCl on spatial learning and memory by using Morris water maze and in situ hybridization in mice. The results showed CsCl 160 mg/kg ip for 4 days, and the mean escape latency was 34 s longer than that of normal control (P＜0.01). In hippocampal tissues, staining for the HCN1 mRNA was stronger in the DG and CA1 region of the hippocampus (P ＜0.05, P＜0.05, when CsCl-administration group was compared with normal group). Our results suggested that CsCl could significantly affect the spatial learning and memory in mice, and HCN channel is involved in the process of learning and memory.

Objective To investigate the effect of GSTT on the release of inflammatory molecules such as TNF-? and IL-1? in rats after ischemia/reperfusion injury. Methods An in vivo model of myocardial ischemia 45min followed by 2h reperfusion was made by reversibly ligating coronary left descending artery. 32 Sprague Dawley (SD) rats were divided randomly to 4 groups, ie: sham-operated group, model group, large and dosage of GSTT. TNF-? and IL-1? concentrations were examined by radioimmunoassay. Results Compared with model group, large dosage of GSTT significantly decreased the contents of TNF-? and IL-1?(P

Objective To investigate the effects of gross saponins from Tribulus terrestris L (GSTT) on myocardial apoptosis, and its related gene bcl-2 and bax expression after hypoxia/reoxygenation in cultured myocardial cells of neonatal rat. Methods After myocardial hypoxia/ reoxygenation model was established by culturing primary myocardial cell of neonatal rat in vivo. The myocardial cells were divided into four groups, the sham-operated group, model group, large- and low-dose of GSTT groups. The apoptotic rate of myocardial cells was determined by flow cytometry, and the expression of bcl-2 and bax was detected by using immuno-histochemical method. Results Both large- and low- dose of GSTT could significantly decrease the apoptotic rate (P

Objective To determine the effect of intramuscular administration of rh-GH on myocardial angiogenesis and the expression of bFGF, VEGF in rats with acute myocardial infarction. Methods Left anterior descending coronary arteries have been ligated in 50 Wistar rats after anesthesia by 10% pentobarbital sodium. 37 rats with AMI survived after the operation. The rats are randomized into rh-GH treatment group (n=19) and the control group (n=18). Treatment group is given rh-GH 0.25 U/kg?d im for three weeks while the control group is given NS im at the same time. All the 37 rats are killed after 3 weeks and the heart specimen were fixed by injecting 10% formalin from the ascending aorta into the coronary arteries. The level of VEGF and bFGF in blood plasma and myocardium as well as the density of newly formed vessels in the peri-infactional area were measured separately. Plasma VEGF and bFGF were measured before experiment and 3 weeks after experiment by enzyme-label method.bFGF and VEGF in myocardiun were semi-quaot fixed by imaging after SP inmuno-histochemical stain. Left ventricle capillary density: 3 angiogenesis sites was identified by 40? or 100? microscopy. F8 labelled microvessels were measured averagely under 200? microscope. Results (1) Plasma VEGF and bFGF in both groups were elevated, and elevation of the treatment group was more remarkable than the control group 69?5 vs 36?4 (P

Objective To investigate the effects of GSTT on expression of inflammatory molecule ICAM-1 and the infiltration of neutrophils in rats after myocardial ischemia/reperfusion (MI/R) injury. Methods A model of myocardial ischemia 45min followed by 2h reperfusion was made by reversibly ligating coronary left descending artery. 32 Sprague Dawley (SD) rats were divided randomly to 4 groups, ie: sham-operated group, model group, large and dosage of GSTT. HE staining was used to observe infiltrating neutophils. The activity of MPO was measured, too. Immunohistochemistry was used to determine ICAM-1 expression in myocardium. Results Light microscopy showed that the infiltration of neutrophils in both large and low dosage of GSTT group was less serious than that in model group. Both large and low dosage of GSTT significantly decreased MPO activity (P

Objective To investigate the effects of gross saponin from Tribulus terrestris (GSTT) on myocardial ischemia/reperfusion (MI/R) injury. Methods A model of myocardial MI/R injury of the myocardium was reproduced by ligation of left descending artery for 45min followed by releasing the ligation for 2 hours in rats. 64 SD rats were divided randomly to 4 groups, i.e. sham-operated group, model group, large and low dose of GSTT groups. HE staining was examined to assess myocardial pathological changes. TTC staining was used to determine myocardial infarction area. Serum contents of lactate dehydrogenase (LDH), creatine phosphokinase (CPK) and malondialdehyde (MDA) were also determined. Results Both large and low dose of GSTT improved myocardial pathological changes after ischemia/reperfusion. Large dose of GSTT reduced myocardial infarction area (P