Background: To evaluate the chemosensitivity to doxorubicin (DOX) in two primary cells derived from a tumor of FVB/N-Trp53tm1Hw1 knockout (KO) mice with TALEN-mediated Trp53 mutant gene, we evaluated the cell survivability, cell cycle distribution, apoptotic cell numbers and apoptotic protein expression in solid tumor cells and ascetic tumor cells treated with DOX. Results: The primary tumor cells showed a significant (P < 0.05) defect for UV-induced upregulation of the Trp53 pro-tein, and consisted of different ratios of leukocytes, fibroblasts, epithelial cells and mesenchymal cells. The ­ IC50 level to DOX was lower in both primary cells ­(IC50 = 0.12 μM and 0.20 μM) as compared to the CT26 cells ­(IC50 = 0.32  μM), although the solid tumor was more sensitive. Also, the number of cells arrested at the G0/G1 stage was significantly decreased (24.7–23.1% in primary tumor cells treated with DOX, P < 0.05) while arrest at the G2 stage was enhanced to 296.8–254.3% in DOX-treated primary tumor cells compared with DOX-treated CT26 cells. Furthermore, apoptotic cells of early and late stage were greatly increased in the two primary cell-lines treated with DOX when compared to same conditions for CT26 cells. However, the Bax/Bcl-2 expression level was maintained constant in the primary tumor and CT26 cells. Conclusions To the best of our knowledge, these results are the first to successfully detect an alteration in chemosensitivity to DOX in solid tumor cells and ascetic tumor cells derived from tumor of FVB/N-Trp53tm1Hw1 mice TALENmediated Trp53 mutant gene.

Abdominal cutaneous nerve entrapment syndrome is one of the abdominal wall-origin pain that develops in anterior cutaneous branches of 7th to 12th thoracic nerves passing through fibrous ring in rectus abdominis. Number of this condition has been misdiagnosed to visceral dysfunctions; therefore, delayed diagnosis leads to waste of cost and time. Injection of local anesthetics is a kind of useful technique for the purpose of the confirmed diagnosis with treatment in abdominal wall pain. Recently, the accuracy and safety of local anesthetics injection to muscle or nerve are significantly improved than the past times since ultrasound has been accepted as a substantial device in clinical area. Here, we report a case of abdominal cutaneous nerve syndrome, treated by ultrasound-guided nerve block accompanied with medical treatment.
Abdominal Wall ; Anesthetics, Local ; Delayed Diagnosis ; Diagnosis* ; Nerve Block ; Nerve Compression Syndromes* ; Rectus Abdominis ; Thoracic Nerves ; Ultrasonography*