Diabetes mellitus (DM) remains a great challenge in treatment due to pathological complexity. It has been proven that phytomedicines and natural medicines have prominent antidiabetic effects. This work aimed to develop selenium-layered nanoparticles (SeNPs) for oral delivery of mulberry leaf and extracts (MPE), a group of phytomedicines with significant hypoglycemic activities, to achieve a synergic antidiabetic effect. MPE-loaded SeNPs (MPE-SeNPs) were prepared through a solvent diffusion/ reduction technique and characterized by particle size, potential, morphology, entrapment efficiency (EE) and drug loading (DL). The resulting MPE-SeNPs were 120 nm around in particle size with EE of 89.38% for rutin and 90.59% for puerarin, two marker components in MPE. MPE-SeNPs exhibited a slow drug release and good physiological stability in the simulated digestive fluid. After oral administration, MPE-SeNPs produced significant hypoglycemic effects both in the normal and diabetic rats. intestinal imaging and cellular examinations demonstrated that MPE-SeNPs were provided with outstanding intestinal permeability and transepithelial transport aptness. It was also revealed that MPE-SeNPs could alleviate the oxidative stress, improve the pancreatic function, and promote the glucose utilization by adipocytes. Our study provides new insight into the use of integrative nanomedicine containing phytomedicines and selenium for DM treatment.

Objective To explore the clinical effect of manipulation treatment combined with auricular point sticking on vertebral artery cervical spondylopathy. Methods 120 patients with vertebral artery cervical spondylopathy were randomly divided into 3 groups, 40 cases in each. The comprehensive group received manipulation treatment and auricular point sticking. The manipulation treatment group received manipulation treatment only and the auricular point sticking group received auricular point sticking only. The peak systolic velocity (Vp), end diastolic peak flow velocity (Vd), mean peak flow velocity (Vm) of the vertebral-basilar artery were checked before and after treatment. Results The clinical cure rates (80%) was higher in the comprehensive group than in the manipulation treatment group (52.5%) and in the auricular point sticking group (47.5%) (P<0.01). The Vp, Vd, Vm in 3 groups improved obviously after treatment (P<0.05), especially in the comprehensive group (P<0.01). Conclusion Manipulation treatment with auricular point sticking is more effective on vertebral artery cervical spondylopathy than simply manipulation treatment or auricular point sticking.