Objective:To explore the feelings and experiences of young breast cancer patients' fertility intension and concern from the perspective of need theory, pay attention to the fertility needs of young breast cancer patients, so as to provide a basis for making personalized clinical decision and support.Methods:From April to July 2020, purpose sampling was used to select 12 young breast cancer patients from the Sun Yat-sen University Cancer Center for semi-structured interviews. The Colaizzi 7-step analysis method was used to encode, classify, and refine topics.Results:This study drew up an interview outline based on the need theory. This study conducted interviews with young breast cancer patients to extract 4 levels of themes related to the feelings and experiences of the fertility intension and concern, which were the basic needs and fertility concerns under the self-efficacy theory, and fertility intension and concern at the level of need for belonging and love, and self-realization needs and fertility concerns based on the sublimation of social roles, and fertility choice and concerns under the background of traditional culture.Conclusions:From the perspective of need theory, role realization and traditional culture have obvious influences on young breast cancer patients' fertility intension and concern. When making diagnosis, treatment and nursing decisions, medical and nursing staff need to give personalized fertility information support and nursing guidance based on the patient's social and cultural background.

Objective To analyze the diversity of salivary bacteria in patients with nonalcoholic fatty liv-er disease(NAFLD group)and healthy control group(HC group). Methods Saliva samples were collected from 24 patients with nonalcoholic fatty liver disease and 22 healthy control.Genomic DNA of the samples was extracted and extended to 16S rRNA V4-V5 hypervariable region after PCR with Illumina high-throughput sequencing tech-nology.All the data,including operational taxonomic units,diversity indexes and species annotation,etc.was ana-lyzed. Results 39118 optimized sequences in the saliva of each group were obtained,including nonalcoholic fatty liver group clustering OTUs about 308 ± 48 and healthy control group clustering OTUs about 305 ± 53.The diversi-ty analysis of NAFLD group and HC group showed that there was little difference in diversity of the whole salivary bacteria.The data of the species analysis of the saliva bacteria in two group indicate that the composition was simi-lar and the relative abundance was different in phylum and genus.In NAFLD group,the ratio of the abundance of firmicutes(20.42%)increased and the proportion of bacteroides(36.75%)decreased in phylum. Genus of the NAFLD group,the ratio of fusobacterium,porphyromonas and veillonella increased significantly,and the ratio of prevotella and[Prevotella]decreased.Conclusion In the NAFLD group,the abundance of firmicutes increased, and the abundance of the bacteria decreased.It was speculated that the imbalance of the salivary bacteria is associ-ated with nonalcoholic fatty liver disease.

Objective To investigate the endoscopic prevalence of erosive esophagitis (EE) among 13 hospitals in Guangdong province of China. Methods Retrospectively reviewed all the cases (63459 cases) that received oesophagogastrodeuodenoscopy in 13 main hospitals in Guangdong province of China in 2003. Los Angeles criteria for classification of erosive esophagitis were employed as the basis of analysis. Results One thousand two hundreds and sixty-three patients (age range 3-90yr, mean 50. 2 ?17. 1 ) were found to have EE. The overall prevalence of EE was 1. 99% (1263/63459). The prevalence of EE in A, B, C, and D grade were 0. 94% , 0. 69% , 0. 21% and 0. 14% respectively. Age correlated positively on endoscopic grading of EE (F=22. 932, P