Introduction: The complete blood count (CBC) is a frequently performed laboratory test today. This study evaluated the effects of temperature and sample storage time on parameters of CBC which could produce misleading results of clinical significance. Methods: In a cross-sectional study, CBC was checked in 20 randomly selected out-patients and baseline measurements were analyzed using the XN-2000 (Sysmex, Kobe, Japan) fully automated hematology analyzer. CBC was done all samples of storage at room temperature. Values were checked at time intervals of 0, 6, and 24 hr. Results: Among CBC parameters, white blood cell, red blood cell, hemoglobin, mean cell hemoglobin (MCH), neutrophils and lymphocytes were stable at time up to 6 h. Hematocrit increased between 0 and 24 hours, averaging 41.5% and 45.2%, respectively. MCV, RDW-SD, and RDW-CV increased between 0 and 24 hours. The mean value was statistically significant. There were 85.6fL/ 93.4fL (p<0.001), 40.7fL /48.2fL (p<0.001), 13.1% and 14.2% (p<0.05), respectively.
However, the MCHC was affected by time differences. (p <0.001 at 0 and 24 hours, p <0.001 at 3 and 24 hours). Platelet PDW, MPV, and P-LCR values increased between 0 and 24 h, respectively. Conclusion Whole blood samples were stored at room temperature for 24 hours for CBC tests, there were statistically significant differences in the size of red blood cells and platelets.

Background: In the world the second out of top 5 diseases is gastroentorogical diseases, particularly hepatocholecystic diseases. LoniceraaltaicaPall, Menthaarvensis are contained in “Antomen” and were used in traditional medicine hepatocholecystic inflamations, oedema, gastric diseases. Objective: To study Antomen’s effect on rats with acute hepatic inflamation. Methods: 40 wistar rats weighing 180-220 g were divided into 4 groups: healthy, control, with standard treatment and antomen’s treatment. Group1 rats were fed with normal standard diet for a week.Group 2 rats (control rats were injected into abdomen with CCL40.8 mg/kg together with olive oil with dosage of 1:1. Control rats were given orally 0.9%NaCl with dosage of 0.8 mg/kg). Group 3 ( rats were injected into abdomen with CCL40.8 mg/kg together with olive oil with dosage of 1:1. Group 3 rats were given orally Carsil with dosage of 40 mg/kg). The last group (rats were injected into abdomen with CCL40.8 mg/kg together with olive oil with dosage of 1:1. Group 4 rats were given orally antomen 200 mg/kg) The experiment continued for a week and on eighth day GPT, GOT and histopathological tests were done. Results:From the result of the study it was determined the preparation has no toxicity. There were no any differences observed in movements, appetite, and color of skin in mise. Group 4 rats were compared with control rats and indications such as GPT- 25.9%, GOT-38%, alkaline phosphatase-22,1% were decreased. Conclution: It can be clearly shows that antomen preparation has hepatoprotective effect.