Objective:Nuclear magnetic resonance hydrogen spectrum(¹H-NMR) was adopted to investigate the effect of dry-cold environment and diet on urine and plasma metabolites of rats,in order to find possible biomarkers and their related metabolic pathways. Method:The dry-cold environment and diet were used as a stressors to intervene rats for 3 weeks,urine and plasma samples were collected.¹H-NMR combined with multivariate data analysis were used to identify relevant metabolic markers and analyze their metabolic pathways. Result:The dry-cold environment and diet resulted in significant changes of 23 metabolites in urine and 15 metabolites in plasma;these metabolites were closely related to changes of various metabolic pathways,namely the tricarboxylic acid(TCA) cycle,pyruvate metabolism,glycolysis or gluconeogenesis,valine,leucine and isoleucine biosynthesis,glycine,serine and threonine metabolism,histidine metabolism;glyoxylic acid and dicarboxylic acid metabolism,alanine,aspartic acid and glutamate metabolism. Conclusion:Dry-cold environment and diet conditions can lead to the changes of various metabolic pathways in the body.The metabolomics based on ¹H-NMR has the advantage of explaining the pathophysiological dynamics and overall changes of the body,which is of great significance for exploring the pathogenesis of non-deterministic disease factors.