Objective:To investigate the effects of docetaxel, Carboplatin combined with radical cervical cancer surgery on the expression of serum tumor markers and Th1/Th2 cytokines in patients with cervical cancer.Methods:96 patients with cervical cancer from Aug. 2018 to Aug. 2020 were selected as the study subjects. They were randomly divided into operation group and chemotherapy + operation group according to random number table method. Surgical group underwent radical hysterectomy (laparoscopic hysterectomy + pelvic lymph node dissection), chemotherapy + surgery group received docetaxel + Carboplatin adjuvant chemotherapy before operation. Serum tumor markers [CEA, CA125, CA19-9, squamous epithelium fine] were compared between the two groups. The expression of SCC, Th1/Th 2 cytokines [IFN-gamma, IL-2, IL-4, IL-6] and the occurrence of bone marrow suppression were evaluated.Results:There was no significant difference in serum CEA, CA125, CA19-9 or SCC between the two groups before treatment ( P>0.05), but they were significantly decreased after treatment ( P<0.05). After treatment, IFN-γ [ (20.58±3.52) ; (25.69±3.61) ] and IL-2 [ (24.68±4.46) ; (29.53±5.28) ] were significantly increased in the two groups ( P<0.05). IL-4 [ (17.65±4.12) ; (13.59±4.23) ] and IL-6 [ (24.59±3.85) (31.76±4.42) ] were significantly decreased ( P<0.05). IFN-γ and IL-2 in the acupuncture group were significantly higher than those in the operation group ( P<0.05). IL-4 and IL-6 were significantly lower than those in the operation group ( P<0.05), and serum CEA [ (33.89±5.51) ng/L; (30.46±4.86) ng/L], CA125, CA19-9 [ (42.21±5.23) U/mL; (37.56±5.61) U/mL] and SCC [ (1.12±0.12) μg/mL; (0.96±0.15) μg/mL] were significantly decreased ( P<0.05). The chemotherapy group was significantly lower than the surgery group ( P<0.05), and the effective rate of chemotherapy group was significantly higher than that of the operation group ( P<0.05), and there was no significant difference in myelosuppression between the two groups ( P>0.05) . Conclusion:Docetaxel plus Carboplatin adjuvant chemotherapy before radical operation of cervical cancer can significantly improve the clinical efficacy, reduce the level of serum tumor markers, improve immune function, and do not increase adverse reactions. It is worth promoting.

Objective To evaluate the effect of manual massage on complications after endoscopic foam sclerotherapy injection for the treatment of internal hemorrhoids.Methods Consecutive 113 patients with grade Ⅰinternal hemorrhoids were prospectively enrolled and completed endoscopic foam sclerotherapy.The patients were randomly divided into a massage group(n=65)and a control group(n=68).Massage group performed manual perianal massage,Visual analogue scale(VAS)was used to evaluate perianal pain.The postoperative bleeding,short-term and long-term efficacy were also compared.Results The median VAS of 24 h postoperation was 1.0(0.0,3.0)in massage group,which was significantly lower than 2.0(1.0,4.0)in control group,the difference was statistically significant(P=0.014).The no bleeding rate of one week postoperation was 84.6％in massage group,which was significantly higher than 64.7％in control group,the difference was statistically significant(P=0.009).After 12 weeks,6 months and 12 months of follow-up,there were no significant differences in cure rate and remission rate between the two groups(P＞0.05).Conclusion Manual massage after endoscopic sclerosing agent injection is beneficial to relieve postoperative pain of grade Ⅰ internal hemorrhoids and reduce bleeding.

Objective:To establish an inductively coupled plasma mass spectrometry (ICP-MS) for platinum antineoplastic drugs in the environment.Methods:The platinum antineoplastic drugs in the environmental table were eluted by wiping and collecting pure water, and the supernatant was taken by centrifugation and inductively coupled plasma mass spectrometry for detection.Results:The concentration range of 0-8.0 μg/L was good, the correlation coefficient was 1.000, the detection limit was 0.0006 μg/L, the lower quantitative limit was 0.002 μg/L, the method precision was between 0.9%-1.3%, and the sample standard recovery rate was between 97.0%-98.5%.Conclusion:This method has low detection limit, high accuracy and precision, and simple sample pretreatment, which is suitable for the determination of platinum antineoplastic drugs in environmental tables.

Objective:To establish an inductively coupled plasma mass spectrometry (ICP-MS) for platinum antineoplastic drugs in the environment.Methods:The platinum antineoplastic drugs in the environmental table were eluted by wiping and collecting pure water, and the supernatant was taken by centrifugation and inductively coupled plasma mass spectrometry for detection.Results:The concentration range of 0-8.0 μg/L was good, the correlation coefficient was 1.000, the detection limit was 0.0006 μg/L, the lower quantitative limit was 0.002 μg/L, the method precision was between 0.9%-1.3%, and the sample standard recovery rate was between 97.0%-98.5%.Conclusion:This method has low detection limit, high accuracy and precision, and simple sample pretreatment, which is suitable for the determination of platinum antineoplastic drugs in environmental tables.

Objective:To establish a LC-MS/MS method for determination of paraquat and diquat in plasma and urine samples.Methods:Plasma is precipitated by acetonitrile then diluent with phosphate buffer (pH=7) , urine is diluent with phosphate buffer (pH=7) , then diluent samples extracted with Oasis WCX solid-phase extraction column. Samples were analyzed using LC-MS/MS in multiple reaction monitoring (MRM) mode. The analytical column was XBridge?BEH-HILIC (100 mm×2.1 mm×2.5 μm) and the mobile phase were 100 mmol ammonium formate add 0.5% formic acid and acetonitrile. Paraquat was quantified by internal standard method and diquat by external standard method.Results:The calibration curves of paraquat and diquat were linear in the concentration range of 10.0~120.0 μg/L, the correlation coefficient (r) were 0.9985~0.9994. The limit of detection of paraquat in plasma and urine were 1.98 μg/L and 1.00 μg/L, respectively, the recovery rate were 100.2%~107.3%, the RSD were 1.6%~3.3%. The limit of detection of diquat in plasma and urine were 1.80 μg/L and 2.77 μg/L, respectively, the recovery rate were 85.3%~93.1%, the RSD were 1.8%~5.5%. Conclusion:This method is sensitive and accurate, and can simultaneously determine paraquat and diquat in plasma and urine.

Objective:To establish a LC-MS/MS method for determination of paraquat and diquat in plasma and urine samples.Methods:Plasma is precipitated by acetonitrile then diluent with phosphate buffer (pH=7) , urine is diluent with phosphate buffer (pH=7) , then diluent samples extracted with Oasis WCX solid-phase extraction column. Samples were analyzed using LC-MS/MS in multiple reaction monitoring (MRM) mode. The analytical column was XBridge?BEH-HILIC (100 mm×2.1 mm×2.5 μm) and the mobile phase were 100 mmol ammonium formate add 0.5% formic acid and acetonitrile. Paraquat was quantified by internal standard method and diquat by external standard method.Results:The calibration curves of paraquat and diquat were linear in the concentration range of 10.0~120.0 μg/L, the correlation coefficient (r) were 0.9985~0.9994. The limit of detection of paraquat in plasma and urine were 1.98 μg/L and 1.00 μg/L, respectively, the recovery rate were 100.2%~107.3%, the RSD were 1.6%~3.3%. The limit of detection of diquat in plasma and urine were 1.80 μg/L and 2.77 μg/L, respectively, the recovery rate were 85.3%~93.1%, the RSD were 1.8%~5.5%. Conclusion:This method is sensitive and accurate, and can simultaneously determine paraquat and diquat in plasma and urine.

Objective:To establish a method for determining mercury in blood with direct mercury analyzer.Methods:After the whole blood sample was extracted by solvent and removed by nitric acid, it was then measured by direct mercury analyzer.Results:After optimizing the conditions of the instrument, the linear range was 0.3-60.0 μg/L and the curve correlation coefficient was higher than 0.999. The lower limit of quantitations was 0.3 μg/L and the minimum quantitative concentration was 3.0 μg/L. The recovery and relative standard deviations ( RSD) was 95.2%-97.6% and 1.4%-3.3%. Conclusion:The method is stable, reliable, easy to operate and has high sensitive. It can be used to determine mercury in blood.

Objective:To establish a method for determining mercury in blood with direct mercury analyzer.Methods:After the whole blood sample was extracted by solvent and removed by nitric acid, it was then measured by direct mercury analyzer.Results:After optimizing the conditions of the instrument, the linear range was 0.3-60.0 μg/L and the curve correlation coefficient was higher than 0.999. The lower limit of quantitations was 0.3 μg/L and the minimum quantitative concentration was 3.0 μg/L. The recovery and relative standard deviations ( RSD) was 95.2%-97.6% and 1.4%-3.3%. Conclusion:The method is stable, reliable, easy to operate and has high sensitive. It can be used to determine mercury in blood.

Objective: To establish a solvent desorption gas chromatographic method for determination of Sevoflurane, Isoflurane and Enflurane in the air of the Workplace. Methods: Sevoflurane, Isoflurane and Enflurane were collected with activated carbon tube and desorbed with dichloromethane, separated with DB-1 capillary columns, and then detected with flame ionization detector. Results: The linearity ranges were 1.9-304.8 μg/ml for Sevoflurane, 2.1-300.4 μg/ml for Isoflurane and 1.7-305.2 μg/ml for Enflurane, The correlation coefficient was both >0.999. Their limits of detection were 0.6 μg/ml, 0.6 μg/ml and 0.5 μg/ml, and Their limits of quatification were 1.9 μg/ml, 2.1 μg/ml and 1.7 μg/ml, and their minimum detectable concentrations were 0.1、0.2 and 0.1 mg/m³ per 4.5 L of air. Their relative standard deviations (RSD) were 2.5%-3.0%, 2.3%-3.1% and 2.2%-3.0%. The average desorption efficiencies were 101.1%-103.3%, 100.7%-102.7% and 101.0%-102.9%. The sampling efficiency was both 100%. The breakthrough volume of 100 mg actived carbon was 3.7 mg, 3.4 mg and 3.4 mg. Sevoflurane, Isoflurane and Enflurane in activated carbon tube could be kept at least 10 days at room temperature without significant losses. Conclusion The method shows lower detection limit, high accuracy and precision. It is feasible for determination of Sevoflurane, Isoflurane and Enflurane in the air of workplace.

Objective: To apply GBZ/T298-2017 "Guidelines for occupational health risk assessment of chemicals in the workplace" to conduct an occupational health risk assessment of 2-butoxyethanol exposure in a printing enterprise, in order to protect the laborers' health. Methods: Conducted a worksite survey of occupational health include data collection, site investigation, determination 2-butoxyethanol in workplace air and raw and auxiliary material, determination 2-butoxyacetic acid in urine after shift from May 17 to 24, 2017, using the above results, conducting 2-butoxyethanol occupational health risk assessment. Results: Concentrations of 2-butoxyethanol in the workplace air ranged from 1.2-30.8 mg/m³. Concentrations of 2-butoxyaceticacid in urine ranged from 35.5 mg/L to 563.3 mg/L. The printing shop with the highest concentration of air in the workplace in the enterprise was a medium risk for 2-butoxyethanol occupational health risks, and the binding workshop printer was a low risk for 2-butoxyethanol occupational health risks. The occupational health risk of 2-butoxyethanol in the print shop was medium. Conclusion Occupational health risks of 2-butoxyethanol for companies is medium, which should be included in the evaluation of routine occupational hazards and provide more targeted recommendations based on the action levels.