Objective: To explore the intervention effect of group counseling integrating teadrinking on depression, social avoidance and distress, coping styles and selfesteem among college students, so as to provide references for promoting the psychological health of students. Methods: From March to June 2021, a convenience sampling method was used to recruit 46 college students from a university in Nanjing for intervention project. There were 23 students in intervention group and control group, respectively. The intervention was conducted for 2.5 hours per week across a 6 week period. The intervention group received two sessions of group counseling that incorporated teadrinking experience, and the sessions involved tea knowledge explanation, observation and learning, embodied experience, emotional expression, interpersonal assistance and emotional support, cultural customs and life integration. After the intervention was completed, the control group received onetime group counseling that integrated teadrinking. The Wilcoxon difference test and Mann-Whitney U test were performed to compare the scores of each scale within and between the groups, and the χ2 test was used to compare the detection rates. Results: There were no statistically significant differences in the scores of positive coping, negative coping, selfesteem, social avoidance and distress between the intervention group and the control group (Z=-1.20, -0.33, -0.35, 1.31,P>0.05). There were statistically significant differences in positive coping styles, selfesteem, social avoidance and distress between the intervention group and the control group after the intervention(27.70±5.60，22.05±4.30；30.52±3.63，28.27±4.06；7.43±7.38，13.64±6.79) (Z=-3.31, -2.10, 3.22, P<0.05). The intervention group showed statistically significant differences in social avoidance and distress scores before and after the intervention(11.96±7.47,7.43±7.38) (Z=-2.88), and the depression detection rate decreased(60.9%，30.4%) (χ2=4.29) (P<0.05). There were no statistically significant differences in positive coping, negative coping, selfesteem, social avoidance and distress scores, and depression detection rate between the control group before and after the intervention (Z/χ2=-0.28, -0.42, -1.24, -1.25；1.39, P>0.05). The followup results 1year later showed that there was a statistically significant difference in social avoidance and distress(6.57±6.21,14.16±9.22), and in the detection rates of depression(21.7%,52.2%) between the intervention group and the control group (Z/χ2=2.70,4.57,P<0.05). Conclusions Group counseling integrating teadrinking could improve interpersonal relationships, emotional states, selfesteem levels and coping strategies among college students. Group psychological counseling that incorporates teadrinking can be adopted to improve the mental health status of college students.

Objective:To explore the locations of the lumbosacral nerve roots by use of the magnetic stimulation.Methods:Thirty healthy subjects were studied. The projections of the right L 2 to S 1 intervertebral foramina on their body surfaces were determined manually with ultrasound assistance. Magnetic stimulation was applied to different nerve root segments to induce compound muscle action potentials (CMAP) in the vastus medialis, tibialis anterior, and gastrocnemius muscles of the lower limbs. The changes in latency, amplitude, and motor threshold were observed. Results:Magnetic stimulation on the L 2-L 3 segment resulted in a significant direct excitation of the vastus medialis. That on the L 5-S 1 segment evoked a significant direct excitatory effect on the tibialis anterior and gastrocnemius, with a motor threshold below 40%, an amplitude exceeding 1mV, and many effective responses. However, during the magnetic stimulation on the L 4 segment, the amplitude of the vastus medialis was above 1mV, with no significant differences in the number of effective responses among the muscle groups. Moreover, there was a stepwise change in the latency of effective muscle responses to magnetic stimulation at different segments. The CMAP latencies of 12+ ms for the tibialis anterior and 13+ ms for the gastrocnemius indicated activation of the L 5 and L 4 nerve roots, respectively, while those of 6+ ms, 7+ ms, and 8+ ms for the vastus medialis suggested activation of the L 4, L 3, and L 2 nerve roots, respectively. Conclusions:Based on the responses (CMAP latency, amplitude and motor threshold) of the vastus medialis, tibialis anterior and gastrocnemius to magnetic stimulation at different L 2 to S 1 segments, the spinal nerve root segments responsible for innervation can be inferred.

It is difficult to accurately distinguish pharyngitis caused by Group A Streptococcus(GAS) from other pathogens according to the clinical presentation alone, which cannot effectively guide the rational use of antimicrobials.The pharyngeal swab culture, rapid antigen detection test, nucleic acid test, and blood test can help definitively diagnose GAS pharyngitis.However, there are differences in different guidelines on who the laboratory test methods are intended for, interpretation of laboratory test results and so on.This article summarizes and analyses the laboratory diagnostic modalities and their characteristics, as well as recommendations for GAS pharyngitis in different guidelines to provide references for the clinical diagnosis, antimicrobial treatment, and further study of GAS pharyngitis.

Objective:To explore the molecular mechanism of dexmedetomidine(Dex)protecting ischemia/reperfusion(I/R)myocardium.Methods:Wild type mice were grouped into control(Control)group,sham operation(Sham)group,WT I/R group,WT Dex group,and Dectin-1 knock out mice were grouped into KO I/R group and KO Dex group in the in vivo study(n=6).TTC stain-ing was used to determine the myocardial infarction area(%)of the above six groups of mice.HE staining and pathological analyze was used to determine the myocardial injury.Serum TNF-α,IL-6 and IL-10 levels in mice were detected by ELISA.Flow cytometry(FCM)was used to count and sort of infiltrating M2 macrophages and neutrophils in myocardium.qPCR assay was used to determine the Dectin-1 mRNA expression in the above sorted cells.Results:TTC results showed that there was no myocardial infarction in the mice of Control group and Sham group.Compared with the WT I/R group,the infarct volume was significantly lower in WT Dex group,KO I/R group and KO Dex group(P<0.05).Compared with the KO I/R group,the infarct volume was reduced in KO Dex group(P<0.05).The results of HE staining showed that the myocardial fibers of the WT I/R group of mice were disorderly arranged,with a large number of broken myocardial fibers,while the myocardial fibers of the WT Dex group,KO I/R group and KO Dex group of mice had a little breakage,the structural damage was not significant,and the myocardial arrangement was relatively neat.The degree of myocardi-al injury of mice in KO Dex group were less than that in KO I/R group mice.ELISA results showed that compared with Sham group,the serum TNF-α and IL-6 levels of the mice in WT I/R group were significantly increased,and the IL-10 level was significantly de-creased.Compared with WT I/R group,serum TNF-α and IL-6 levels of the mice in WT Dex group and KO I/R group were significant-ly decreased,and IL-10 level was significantly increased.Compared with KO I/R group,the serum TNF-α and IL-6 levels of the mice in KO Dex group were significantly decreased,and the IL-10 level was significantly increased(P<0.05).FCM cell counting results showed that compared with Sham group,a large number of M2 macrophages and neutrophils were infiltrated in the myocardium of WT I/R group of mice(P<0.05).Compared with WT I/R group,the M2 macrophages and neutrophils infiltrated in the myocardium were significantly decreased in WT Dex group,KO I/R group and KO Dex group of mice(P<0.05).While there was no significant differ-ence between the KO I/R group and the KO Dex group mice(P>0.05).qPCR results showed that compared with Sham group,the ex-pression level of Dectin-1 mRNA in the myocardial infiltrated M2 macrophages and neutrophils were significantly up-regulated in WT I/R group of mice(P<0.05).While compared with WT I/R group,the expression level of Dectin-1 mRNA in Dex group of mice was sig-nificantly lower(P<0.05).Mice in KO I/R group and KO Dex group did not express Dectin-1.Conclusion:The protective mecha-nisms of Dex preconditioning on I/R injured myocardium involves reducing the infiltrating number of M2 macrophages and neutrophils in myocardium after I/R injury,which may be achieved by inhibiting the expression of Dectin-1.

Objective:To summarize the best evidence for non-pharmacological management of restless leg syndrome (RLS) in patients with chronic kidney disease.Methods:Based on the "6S" evidence model, a computer search was conducted on relevant domestic and international guideline websites and databases, including Guidelines International Network, National Guideline Clearinghouse, Registered Nurses' Association of Ontario, Medlive, Chinese Medical Association Nephrology Branch, National Kidney Foundation website, BMJ Best Practice, UpToDate, PubMed, Cochrane Library, China Biology Medicine disc, and China National Knowledge Infrastructure. The search period was from establishing the databases to September 30, 2023. Two researchers conducted a literature quality evaluation and summarized evidence.Results:A total of 11 articles were included, including two guidelines, two clinical decision-making articles and seven systematic reviews. A total of 30 pieces of evidence were summarized from nine aspects, such as evaluation and management, avoidance of triggering factors, behavioral strategy changes, physical therapy, change in dialysis methods, exercise therapy, surgery, traditional Chinese medicine external treatment, and health education.Conclusions:This study summarizes the best evidence for non-pharmacological management of restless leg syndrome in patients with chronic kidney disease. Medical staff must select and apply the best evidence in a targeted manner based on clinical situations.

Objective To prepare rabbit polyclonal antibody specifically against human lactate dehydrogenase C4 (LDHC4). Methods Site-directed mutation was performed by PCR to generate the mutated LDHC gene, and the mutated gene was ligated into the pET-28a vector to form the pET-28a-LDHC recombinant expression vector. The recombinant vector was introduced into E. coli BL21 (DE3), and LDHC4 protein was obtained by induced expression. The recombinant protein was used as an antigen to immunize New Zealand rabbits, and the antiserum was obtained after three boosted immunizations. The titer of the antiserum against LDHC4 were detected by ELISA. Western blot was used to detect the specificity of the antiserum, and immunohistochemistry was used to detect the expression of LDHC4 in human triple-negative breast cancer tissue. Results A specific rabbit anti-human LDHC4 polyclonal antibody was obtained with an antibody titer of 1:51 200. The antibody can be used for Western blot and immunohistochemistry. Conclusion The specific rabbit anti-human LDHC4 polyclonal antibody is successfully prepared.
Humans ; Rabbits ; Animals ; Escherichia coli/genetics* ; Antibodies ; Enzyme-Linked Immunosorbent Assay ; L-Lactate Dehydrogenase/metabolism* ; Blotting, Western ; Antibody Specificity

BackgroundMental illness during pregnancy has become a major public health problem in China over the recent years， and depression is the most common psychological symptom during pregnancy. Current research efforts are directed towards the therapy on prenatal depression， whereas the construction of prediction model for prenatal depression risk has been little studied. ObjectiveTo construct a simple model for predicting the risk of prenatal depression， thus providing a valuable reference for the prevention of maternal depression during pregnancy. MethodsA total of 803 pregnant women attending three hospitals in Nanchong city were consecutively recruited from May 2021 to February 2022. A self-administered questionnaire was developed for the assessment of social demographic variables， obstetrical and general medical indexes and psychological status of all participants， and Self-rating Depression Scale （SDS） was utilized to screen for the presence of maternal depression. Subjects were randomly assigned into modelling group （n=635） and validation group （n=168） at the ratio of 8∶2 under simple random sampling with replacement. The candidate risk factors of maternal depression during pregnancy were screened using binary Logistic regression analysis， and the predictive model was constructed. Then the performance of the predictive model was validated using receiver operating characteristics （ROC） curve. Results① Lack of companionship （β=-0.692， OR=0.501， 95% CI： 0.289~0.868）， low mood during the last menstrual period （β=-1.510， OR=0.221， 95% CI： 0.074~0.656）， emotional stress during the last menstrual period （β=-1.082， OR=0.339， 95% CI： 0.135~0.853）， unsatisfactory relationship between mother-in-law and daughter-in-law （β=-1.228， OR=0.293， 95% CI： 0.141~0.609）， and indifferent generally relationship between mother-in-law and daughter-in-law （β=-0.831， OR=0.436， 95% CI： 0.260~0.730） were risk factors for prenatal depression in pregnant women （P<0.05 or 0.01）. ② Model for predicting the prenatal depression risk yielded an area under curve （AUC） of 0.698 （95% CI： 0.646~0.749）， the maximum Youden index was 0.357 in modelling group with the sensitivity and specificity was 0.606 and 0.751， and an AUC of 0.672 （95% CI： 0.576~0.767） and maximum Youden index of 0.263 in validation group with the sensitivity and specificity of 0.556 and 0.707. ConclusionThe simple model constructed in this study has good discriminant validity in predicting of the risk of prenatal depression. ［Funded by Nanchong Social Science Research Project of the 14th Five-Year Plan （number， NC21B165）］

Objective? To explore the differences between communication demands of tracheal intubation patients and cognition nurses in intensive care unit (ICU). Methods? We selected 105 tracheal intubation patients after the cardiac surgery and 19 nurses provided nursing for them as subjects at Peking University First Hospital by convenience sampling. All of the patients and nurses were investigated with the communication demands scale of tracheal intubation patients, and the differences between them were compared. Results? The total score of demands of tracheal intubation patients was (99.96±24.93) lower than that (123.11±19.48) of cognition of nurses with a statistical difference (P＜0.05). The highest communication demand of patients was the item of "I want to know the time of tube drawing" with (3.33±1.09) for the score ranked fourth in cognition of nurses. The scores of items in demands of love and belonging as well as self-esteem of patients ranked more than the tenth. Conclusions? There are differences between communication demands of tracheal intubation patients and cognition of nurses in ICU especially in physiological needs, safety needs as well as the need of love and belonging. Encouraging patients to express intentions, understanding patients' intentions and avoiding forming the fixed thinking can carry out nursing service better so as to provide the best scientific basis for patients' disease recovery.

Objective? To explore the integrated doctor-nurse quality nursing service model in daytime surgery for pediatric strabismus. Methods? Totally 2 880 patients receiving daytime surgery for binocular strabismus correction under general anesthesia in Shenzhen Ophthalmology Hospital were selected using convenient sampling. Totally 1 470 patients admitted from January to June 2018 were included into the control group, while another 1 410 patients admitted between July and December 2018 were enrolled in the observation group. Patients in the control group received conventional nursing care, whereas patients in the observation group received integrated doctor-nurse nursing services. Patient＇s satisfaction, incidence rate of postoperative infection and accidental injury while restoring vision were compared between the two groups. Results? Patient＇s satisfaction scored (99.17±0.52) after intervention in the observation group, higher than that in the control group, which was (93.38±1.91), and there was statistically significant difference between the two groups (t=109.446; P＜0.01). The incidence rate of postoperative infection in the observation group was 0.21%, lower than that in the control group, which was 1.36%, and there was no statistically significant difference between the two groups (χ2=11.97; P＜ 0.01).There was no accidental injury caused while restoring vision in the observation group, while 1 patient＇s finger was cut when restoring vision, and there was statistically significant difference between the two groups (P＞ 0.05). Conclusions? The integrated doctor-nurse quality nursing service model was significantly effective in daytime surgery for pediatric strabismus, which offers a reference for nursing care for patients receiving daytime surgery.

Objective To analyze the correlative differences in the cardiac surgery for patients with end-stage renal disease (ESRD) and non-nephropathy disease, and explore the cardiac perioperative nursing for patients with ESRD. Methods A single-center retrospective comparative analysis was conducted in patients with ESRD (ESRD group, 26 cases) and non-nephropathy disease (control group, 26 cases) treated with cardiac surgery from January 2004 to December 2016 in Peking University First Hospital. Postoperative laboratory indexes, the changes of indexes in the monitoring room and the blood transfusion were compared between two groups. Results At 0, 1 and 2 d after the surgery, hemoglobin levels of patients in ESRD group were significantly lower than those in the control group; creatinine and urea nitrogen levels in ESRD group were significantly higher than those in the control group (P< 0.01). Postoperative serum potassium levels in ESRD group were significantly higher than those in the control group (P<0.01). The amount of erythrocyte and platelet transfusion in ESRD group were higher than those of control group (P<0.01). There was no statistically significant difference between the ESRD group and the control group in the ventilator time, 24-hour pleural drainage and total thoracic drainage (P> 0.05). The duration of stay in ICU and postoperative hospital stay were (101.62±49.10) h and (28.23±20.44) d in the ESRD group, which were significantly longer than those in the control group [(71.35±33.10) h, (16.58±8.43) d]. Conclusions ESRD patients have a higher risk in the cardiac surgery when compared with patients with non-nephropathy disease. Therefore, nurses should pay attention to the perioperative electrolyte balance and infusion of blood products, as well as the prevention of postoperative complications and psychological nursing, to help patients with better prognosis.