ObjectiveThis study aims to investigate the therapeutic effects of Wenweishu granule （WWSG） on functional dyspepsia （FD） with spleen-stomach deficiency cold syndrome in rats by integrating network pharmacology， molecular docking， and animal experiments. MethodsActive components and corresponding targets of WWSG were collected from the Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform （TCMSP） and the Bioinformatics Analysis Tool for Molecular Mechanism of Traditional Chinese Medicine （BATMAN-TCM）. Disease-related targets for FD with spleen-stomach deficiency cold syndrome were screened using GeneCards and the Integrative Pharmacology-based Research Platform of Traditional Chinese Medicine （TCMIP）. Core therapeutic targets were identified via Cytoscape and validated by molecular docking. A rat model of FD with spleen-stomach deficiency cold syndrome was established using vinegar gavage combined with tail-clamping. The rats were randomly divided into a model group， low-， medium-， and high-dose WWSG groups （2.0， 4.0， 8.0 g·kg^-1）， a domperidone group （3.0 mg·kg^-1）， a Fuzi Lizhong pillwan （0.8 g·kg^-1）， and a normal control group （n=10 per group）. Drugs were administered once daily by gavage for 14 consecutive days. After treatment， body weight， symptom scores， and gastrointestinal motility indices were recorded. Gastric and duodenal pathologies changes were observed via hematoxylin-eosin （HE） staining. Brain-gut peptides were measured in serum and tissue using enzyme-linked immunosorbent assay （ELISA）. Immunohistochemistry and Western blot were performed to assess stem cell factor （SCF） and receptor tyrosine kinase （c-Kit） protein expression in gastric tissues. ResultsA total of 305 drug targets， 1 140 disease targets， and 116 overlapping targets were identified. Cytoscape analysis revealed 104 core targets. Enrichment analysis indicated that the SCF/c-Kit signaling pathway was the key mechanism. Molecular docking confirmed a strong binding affinity between active components of WWSG and SCF/c-Kit proteins （binding energy<-5.1 kcal·mol^-1）. Compared with the normal group， model rats exhibited slower weight gain （P<0.05）， reduced gastric emptying and intestinal propulsion （P<0.01）， mild gastric mucosal shedding， duodenal inflammatory cell infiltration， decreased levels of gastrin （GAS）， 5-hydroxytryptamine （5-HT）， and vasoactive intestinal peptide （VIP） （P<0.05， P<0.01）， and elevated somatostatin （SS） expression （P<0.05， P<0.01）. WWSG treatment ameliorated weight gain， symptom scores， and low-grade inflammation in gastric/duodenal tissues. High-dose WWSG significantly improved gastric emptying and intestinal propulsion， upregulated GAS， 5-HT， and VIP， and downregulated SS expression in serum and tissues （P<0.05， P<0.01）. Immunohistochemistry and Western blot demonstrated that SCF and c-Kit protein expression was decreased in the model group （P<0.05， P<0.01）， which was reversed by WWSG intervention （P<0.05）. ConclusionWWSG exerts therapeutic effects on FD with spleen-stomach deficiency cold syndrome in rats， potentially by regulating the SCF/c-Kit signaling pathway to enhance gastrointestinal motility.

Cyclodextrin metal-organic framework (CD-MOF) as a highly porous supramolecular carrier could be one of the solutions to the insolubility of isosteviol (STV). The solubility of STV was lower than 20.00 ng/mL at pH 1.0 and pH 4.5, whilst its solubility increased to 20,074.30 ng/mL at pH 6.8 and 129.58 ng/mL in water with a significant pH-dependence. The

Objective:To provide the solution based on electronic authentication service and electronic signature for doctor and nurse in order to guarantee the integrity and authenticity of electronic medical records system(EMRS) in building, writing and storage.Methods: To establish integrated electronic signature platform based on the installation and debugging for the digital signature sever device; and to improve some parts of the database, such as the digital certificate, certificate logging and the integration process of digital signature etc.Results: The integrated electronic signature of EMRS can guarantee the authenticity and reliability of identity when doctor logged system or finished the normal medical work; and it can reduce manual signature process and contribute to finish the automatic signature after doctor or nurse finished EMRS and nursing recorder sheet.Conclusion: The implementation of the electronic signature can ensure the reliability of electronic medical records, achieve the complete electronic file storage for patient medical records, and greatly save manpower and material resources.

Objective:To study the correlation of tumor-associated macrophages infiltration , MMP-2 expression and angiogenesis in colon carcinoma by testing the MMP-2 through immunological methods ,and counting of macrophages and distributing of vessels in colonic adenocarcinoma at different differential degrees .Methods: Analyze the infiltration of tumor-associated macrophages (TAMs),and count blood capillaries in 79 patients with colon carcinoma by immunohistochemical staining ,detect the expression of MMP-2 in macrophages by immunological methods .Analyze the relationship among numbers of macrophages and vessels , clinical pathology and expression of MMP-2.Results: Macrophages in colon carcinoma were labeled to brown yellow by murine monoclonal antibody to human.The number of macrophages was markedly higher than normal group (F=412.04,P<0.05); Variance of macrophages number had statistical significance in differentiatial degrees and Duck ′s staging of colon carcinoma ( t=10.80 and F=412.04,P<0.05);variance of microvessel density(MVD) had statistical significance in Duck′s staging of colon carcinoma(t=7.35, P<0.05 ) ,MVD in colon carcinoma patients with lymphatic metastasis was higher than patients without lymphatic metastasis ( t=6.77 , P<0.05).Expression of MMP-2 in colon carcinoma showed strong positive.Expression of MMP-2 in patients with lymphatic metastasis was higher than patients without lymphatic metastasis ( t=10.91 , P<0.05 ) . There was a positive relationship among MMP-2 expression,number of tumor-associated macrophages(TAMs) and MVD(r=0.451,0.672,P<0.05,respectively).Conclusion:MMP-2 and TAMs correlated closely with the development of colon carcinoma .TAMs in colon carcinoma can promote the angiogenesis ,growth and metastasis of the tumor by up-regulating the expression of MMP-2.

Objective:The effect of Wireless Analgesia System for the patients with postoperative pain, chronic pain and cancer pain in Anesthesiology Department will be discussed in this article. Methods:With the wireless signal being sent from the analgesia terminal transmitter to the base station of analgesia control, the analgesia pump can be connected with wireless analgesia terminal. Drugs can be delivered persistently or intermittently to keep the plasma concentration in an appropriate level according the vested parameters from the wireless system of analgesic delivering. Results:The information from the patients in the process of analgesia can be processed and saved by the system. According to the information of running and alarm displaying at the monitoring station, the analgesia records can be kept automatically. Conclusion:The satisfaction degrees on analgesia treatment can be improved, and the burden of work for anesthesiologists can be lessened as well, which improve the quality of medical services and promote the development of informatization in anesthesiology department.

Objective To observe the prevalence of anionic trypsinogen (PRSS2) gene G191R mutation in patients with acute pancreatitis (AP) and chronic pancreatitis (CP),and to investigate the effect of PRSS2 gene G191R mutation on susceptibility to pancreatitis.Methods The blood samples of 82 patients with acute pancreatitis,73 patients with chronic pancreatitis and 138 healthy subjects were collected,and genomic DNA was extracted.Nest PCR were performed to amplify PRSS2 gene and restriction fragment length polymorphism (RFLP) was followed by using Hpy188Ⅲ to distinguish the G191R mutation.DNA sequencing analysis was performed to confirm the mutation status.Results The size of nest PCR products was 436 bp.RFLP2 produced 309 bp and 127 bp fragments,which were resulted from PRSS2 gene G191R mutation (GGA →AGA).DNA sequencing analysis of the PCR products further confirmed the PRSS2 gene G191R mutation.Five of eighty-two(6.1％) patients with acute pancreatitis had PRSS2 gene G191R mutation (OR=0.682,95％ CI 0.231 ～ 2.010); one of seventy-three (1.4％) patients with chronic pancreatitis had the mutation (OR =0.145,95％ CI 0.019 ～ 1.145),and the corresponding value in healthy group was 8.7％ (12/138).The G191R mutation rate in patients with chronic pancreatitis was significantly lower than that in healthy group (x2 =0.432,P =0.035),but the G191R mutation rates were not significantly different between AP group and healthy group (x2 =0.487,P =0.485).Conclusions PRSS2 gene G191R mutation facilitates the degradation of anionic trypsin,and may reduce the incidence of chronic pancreatitis.

Objective The aim of this paper was to develop the Mobile Nutrition Order System based on the technology of hospital information system to adapt the nutrition and food security management model in the new situation, so that the unified informa-tization management of diet for patients could be implemented by the Department of Nutrition. Methods The Mobile Nutrition Order System, with the functions of information entry, nutrition recipe, order making and data transmission, could be constructed by the com-bination of technologies between informatization and digital order. Results The automation of nutrition order in hospital was a-chieved, and the nutrition recipe and nutritional therapy could be realized in hospital, the effect was very obvious. Conclusion Since the application of Mobile Nutrition Order System, the labor intensity of servers has been reduced, saving working hours effective-ly.Meanwhile, the management of nutritional therapy was improved and the quality of logistics services in hospital and nutritional ther-apy ascend to a new high.

Objective: To design a set of digital medical record MIS. Methods: According to the problems of paper-based medical records management, the protocol of digital medical record MIS using micro-digital imaging technology was proposed. Results:It realized electronic medical record storage, the front page information of medical records, medical record circulation and statistics of medical information. Conclusion: The storage of paper based medical record was effectively compressed, the information level of medical record management was clearly promoted after using the digital medical record MIS. It realized the conservation and usage for current medical records, guaranteed information security and data sharing etc.

Objective: Through the application of network and information technologies, the functions in the Anesthesia Department including equipments control, data collection, surgery demonstration, remote diagnosis, multimedia management, integration of information systems can be implemented. Methods: Through the combination of the reconstruction in Anesthesia Department with network information technology, a variety of devices can be linked as far as possible to establish the clinical information system, service system and management system in digital operating Anesthesia Department. Results:The working process of clinical information systems can be standardized by integrating and sharing the data. The systems of medical service become smooth and interactive, which brings the benefits for doctors, nurses and patients. The resources in Anesthesia Department can be utilized comprehensively, which promotes the hospital management and improves the medical services. Conclusion:As an inevitable trend for the development of digital hospital, the construction and application of the digital Anesthesia Department promotes the development of anesthesiology and information construction for Anesthesia Department, which creates economic and social benefits for hospital and improves the core competitiveness for hospital.

Objective To explore the relationship of catheter-directed thrombolysis and deep venous thrombosis.Methods We analyzed 2267 consecutive central venous catheter attempts from January to December in2006.Results 18 patients had deep venous thrombosis,and several studies have identified factors associated with mechanical complications of central venous catheter.These factors include illness,the use of drug,mobility.Conclusion It is very important to prevent and cure deep venous thrombosis and decrease incidence of death of knowing many risk factors and clinical symptoms of catheter-directed thrombolysis.