Objective:To explore the reform path of clinical laboratory technical talents training in local universities under the background of "New Medicine".Methods:The present situation of medical laboratory technical personnel training under the background of "New Medicine" was analyzed, and the teaching mode, teaching platform and practical teaching were reformed according to the reality of Guizhou Medical University.Results:An open education system of "healthcare-education collaboration and academia-industry alliances" and the talent training mode of "three-oriented drive, four-sided integration" had been formed, which improved the training quality and provided a large number of qualified medical laboratory technical undergraduate talents to the grass-roots of Guizhou Medical System.Conclusion:The reform and practice of applied talent training in Guizhou Medical University can be used as a reference for local colleges and universities to educate applied medical laboratory technical talents.

Objective:To discuss the effect and prognostic factors of immediate facial nerve rehabilitation in malignant parotid gland tumor surgery.Methods:The patients with malignant parotid gland tumor who underwent facial nerve rehabilitation surgery in Peking University School and Hospital of Stomatology from January 2004 to July 2020 were retrospectively analyzed. Their demographic characteristics, preoperative and postoperative facial nerve function according to the House-Brackmann (HB) facial nerve grading system from HB grade Ⅰ to HB grade Ⅵ, injury sites of facial nerve including the trunks injury and branches injury, surgical timing including immediate rehabilitation and elective rehabilitation and rehabilitation methods such as facial nerve anastomosis, nerve transplantation, nerve transfer and muscle flap suspension were recorded. Patients were divided into two groups: satisfactory outcomes with HB grades Ⅰ-Ⅲ and unsatisfactory outcomes with HB grades Ⅳ-Ⅵ, and the factors that may affect the prognosis of facial nerve function were statistically analyzed. T-test was used to compare measurement data, chi-square test or Fisher’s precision probability test was used to compare count data. P<0.05 was considered statistically significant. Results:Eighty-nine patients were included in the study, including 50 males and 39 females, aged 9-82 years, the median age was 49.0 years. Preoperative facial nerve function outcomes were HB Ⅰ ( n=57), HB Ⅱ ( n=5), HB Ⅲ ( n=8), HB Ⅳ ( n=2), HB Ⅴ ( n=15) and HB Ⅵ ( n=2). Fifty-seven patients underwent immediate rehabilitation and 32 patients received elective rehabilitation. In 53 cases, the main trunk and/or the temporal and cervical trunk were injured, and 36 cases were branches injury. Facial nerve anastomosis was performed in 35 cases, 39 cases required nerve transplantation, 12 cases underwent nerve transfer, and 3 cases underwent muscle flap suspension. The follow-up time was 6-72 months. Postoperative facial nerve function outcomes were HB Ⅰ ( n=6), HB Ⅱ ( n=17), HB Ⅲ ( n=26), HB Ⅳ ( n=27), and HB Ⅴ ( n=13). Forty-nine cases were classified as group with postoperative HB grades Ⅰ-Ⅲ, and 40 cases were classified as group with postoperative HB grades Ⅳ-Ⅵ. Statistical analysis showed that there was no significant difference in terms of age and gender between two groups ( P>0.05). Among the patients with postoperative HB grades Ⅰ-Ⅲ, 73.5% (36/49) of the patients had immediate rehabilitation, 36.7% (18/49) of the patients had injury of the nerve trunks, 89.8% (44/49) of the patients had preoperative HB grades Ⅰ-Ⅲ, and 59.2% (29/49) of the patients had nerve anastomosis. Among the patients with postoperative HB grades Ⅳ-Ⅵ, 52.5% (21/40) of the patients had immediate rehabilitation, 87.5% (35/40) of the patients had injury of the nerve trunks, 65.0% (26/40) of the patients had preoperative HB gradesⅠ-Ⅲ, and 15.0% (6/40) of the patients had nerve anastomosis were in the group. There were statistically significant differences between the two groups ( P<0.05 or <0.01). Conclusions:Immediate reconstruction of the facial nerve defect during the operation of malignant parotid gland tumor can obtain better result. Furthermore, injury sites of facial nerve may affect the prognosis of facial nerve function as well.

Objective:To discuss the effect and prognostic factors of immediate facial nerve rehabilitation in malignant parotid gland tumor surgery.Methods:The patients with malignant parotid gland tumor who underwent facial nerve rehabilitation surgery in Peking University School and Hospital of Stomatology from January 2004 to July 2020 were retrospectively analyzed. Their demographic characteristics, preoperative and postoperative facial nerve function according to the House-Brackmann (HB) facial nerve grading system from HB grade Ⅰ to HB grade Ⅵ, injury sites of facial nerve including the trunks injury and branches injury, surgical timing including immediate rehabilitation and elective rehabilitation and rehabilitation methods such as facial nerve anastomosis, nerve transplantation, nerve transfer and muscle flap suspension were recorded. Patients were divided into two groups: satisfactory outcomes with HB grades Ⅰ-Ⅲ and unsatisfactory outcomes with HB grades Ⅳ-Ⅵ, and the factors that may affect the prognosis of facial nerve function were statistically analyzed. T-test was used to compare measurement data, chi-square test or Fisher’s precision probability test was used to compare count data. P<0.05 was considered statistically significant. Results:Eighty-nine patients were included in the study, including 50 males and 39 females, aged 9-82 years, the median age was 49.0 years. Preoperative facial nerve function outcomes were HB Ⅰ ( n=57), HB Ⅱ ( n=5), HB Ⅲ ( n=8), HB Ⅳ ( n=2), HB Ⅴ ( n=15) and HB Ⅵ ( n=2). Fifty-seven patients underwent immediate rehabilitation and 32 patients received elective rehabilitation. In 53 cases, the main trunk and/or the temporal and cervical trunk were injured, and 36 cases were branches injury. Facial nerve anastomosis was performed in 35 cases, 39 cases required nerve transplantation, 12 cases underwent nerve transfer, and 3 cases underwent muscle flap suspension. The follow-up time was 6-72 months. Postoperative facial nerve function outcomes were HB Ⅰ ( n=6), HB Ⅱ ( n=17), HB Ⅲ ( n=26), HB Ⅳ ( n=27), and HB Ⅴ ( n=13). Forty-nine cases were classified as group with postoperative HB grades Ⅰ-Ⅲ, and 40 cases were classified as group with postoperative HB grades Ⅳ-Ⅵ. Statistical analysis showed that there was no significant difference in terms of age and gender between two groups ( P>0.05). Among the patients with postoperative HB grades Ⅰ-Ⅲ, 73.5% (36/49) of the patients had immediate rehabilitation, 36.7% (18/49) of the patients had injury of the nerve trunks, 89.8% (44/49) of the patients had preoperative HB grades Ⅰ-Ⅲ, and 59.2% (29/49) of the patients had nerve anastomosis. Among the patients with postoperative HB grades Ⅳ-Ⅵ, 52.5% (21/40) of the patients had immediate rehabilitation, 87.5% (35/40) of the patients had injury of the nerve trunks, 65.0% (26/40) of the patients had preoperative HB gradesⅠ-Ⅲ, and 15.0% (6/40) of the patients had nerve anastomosis were in the group. There were statistically significant differences between the two groups ( P<0.05 or <0.01). Conclusions:Immediate reconstruction of the facial nerve defect during the operation of malignant parotid gland tumor can obtain better result. Furthermore, injury sites of facial nerve may affect the prognosis of facial nerve function as well.

In order to develop a method to more accurately determine the surgical boundary of extramammary Paget′s disease, reflectance confocal microscopy was used to determine the tumor boundary followed by modified extended excision in 2 cases of pathologically diagnosed extramammary Paget′s disease of the vulva. No residual tumor was observed in the resection margins by postoperative pathological examination at 4 positions (12, 3, 6 and 9 o′clock) , and follow-up showed no recurrence 1 year later.

Objective:To explore the therapeutic effect of adipose-derived stem cells (ADSC) on long-wave UV damage in mouse skin in order to provide ideas for the treatment of skin photodamage.Methods:The inguinal and perirenal adipose tissues of C57BL/6 mice were extracted and processed to obtain mouse ADSCs, and the surface markers, adipogenic and osteogenic differentiation capabilities were identified. The mouse photoaging model was irradiated with the SS-03AB UV illuminator, the total UVB dose was 9.45 J/cm 2, and the total UVA dose was 94.5 J/cm 2. Experimental mice (72 in total) were divided into normal group, model group, DMEM (medium) group and ADSC group, each with 18 mice. In the normal group and model group, the materials were taken two weeks after the end of irradiation. After irradiation, the ADSC group was given a subcutaneous injection of 200 μl ADSC suspension, and the DMEM group was given 200 μl of serum-free medium for treatment, and the materials were taken for pathological staining after 2 weeks. The experimental data was processed by analysis of variance. This study was carried out from August 2018 to July 2019 in the First Affiliated Hospital of Zhengzhou University. Results:The extracted cells were identified as adipose-derived stem cells. HE staining showed that the inflammatory cell infiltration in the ADSC group was significantly reduced compared with the DMEM group ( t=20.649, P<0.001) and the normal group ( t=16.147, P<0.001), and the thickness of the dermis layer was significantly increased. Masson staining showed collagen fibers were arranged neatly and the density increased significantly after ADSC treatment. Conclusions:Subcutaneous injection of ADSC can reduce inflammation, promote collagen tissue proliferation, increase the thickness of the dermis, effectively resist inflammatory damage and collagen breakdown caused by UVB.

Objective:To evaluate the efficacy of postoperative radiotherapy (PRT) for dermatofibrosarcoma protuberans (DFSP).Methods:A systematic review and meta-analysis of articles published before February 23, 2019 were conducted. A total of 655 studies were retrieved consisting of 195 DFSP patients. Among them, 50 cases were assigned into the PRT group and 145 cases in the surgery alone (SA) group. The recurrence rate was statistically compared between two group.Results:Meta-analysis showed that the recurrence rate in the PRT group was significantly lower than that in the SA group (8% vs. 24.1%, OR=0.28, P=0.010). The recurrence rate of patients with positive margins in the PRT group was significantly lower compared with that in the SA group (8% vs. 61.5%, P=0.002). The recurrence rate of patients with negative margins in the PRT group had a decreasing trend than that in the SA group (6% vs. 21.6%, P=0.205). Conclusions:The recurrence rate of surgery combined with PRT is lower than that of SA. The recurrence rate of patients with positive margins is higher than that of those with negative margins. For patients with positive margins, PRT can decrease the recurrence rate. The recurrence rate trends to decline in patients with negative margins after receiving PRT.

Objective:To explore the application of experiential teaching combined with case-based learning (CBL) in the teaching of nursing students in intensive care unit (ICU).Methods:A total of 63 nurses who had internship in the ICU of our hospital from April 2016 to March 2017 were selected as group A, 63 nurses from April 2017 to March 2018 were selected as group B, 63 nurses from April 2018 to March 2019 were selected as group C, and 63 nurses from April 2019 to March 2020 were selected as group D. Group A adopted conventional teaching method, group B adopted conventional teaching method + CBL, group C adopted conventional teaching method + experiential teaching method, and group D adopted conventional teaching method + CBL + experiential teaching method. All of them had been taught for 3 months. The scores of theory and skills examination, humanistic care, supportive communication and critical thinking ability before and after teaching, and satisfaction with teaching mode were compared among the four groups. SPSS 26.0 was used for one-way variance analysis, SNK -q test and χ2 test. Results:The scores of theory and skill examination in group D were higher than those in the other three groups, and those in group B and C were higher than those in group A ( P<0.05). After teaching, the scores of humanistic care, supportive communication and critical thinking ability of the four groups were higher than those before teaching ( P<0.05). After teaching, the scores of humanistic care, supportive communication and critical thinking ability of group D were higher than those of the other three groups ( P<0.05), and those of group B and group C were higher than those of group A ( P<0.05). The satisfaction scores of teaching skills, teaching content and teaching effect in group D were higher than those in the other three groups ( P<0.05), and those in group B and C were higher than those in group A ( P<0.05). Conclusion:On the basis of conventional teaching method, experiential teaching and CBL can improve the performance of nursing students, improve the ability of humanistic care, supportive communication and critical thinking, and improve the satisfaction of nursing students. The combination of the two has a better effect.

The number of H7N9 bird flu cases was high and the situation was grim in guizhou province in 2017. To understand the molecular characteristics of the hemagglutinin gene (HA) and the risk of human infection with avian influenza virus A(H7N9) in Guizhou Province, 2017. Homology, genetic evolution and pivotal sites related to receptor binding regions, pathogenicity and potential glycosylation of 14 avian influenza viruses A(H7N9) were analyzed by a series of bioinformation softwares. It was cleared that there was 95.9%-100% similarity among 14 strains in nucleotide of the HA gene, and there were 96.8%-97.8% and 96.8%-97.9% similarities with vaccine strains A/Shanghai/2/2013 and A/Anhui/1/2013 recommended by WHO, respectively. Phylogenetic analysis showed that 14 HA genes were directly evolved in the Yangtze River Delta evolution branch, but they could be derived from five diffenrent strains. Then 13 of 14 strains cleavage site sequences of HA protein revealed they were low pathogenic avian influenza viruses, while A/Guizhou-Weining/CSY01/2017 was high pathogenic avian influenza virus. Mutation G186V at the receptor binding sites in the HA was found in all 14 strains, and mutation Q226L in 13 strains besides A/Guizhou-Weining/CSY01/2017. All five potential glycosylation motifs in the HA were conservative.

Objective To evaluate the effects of extracts of Semen Coicis (ESC) on a BALB/c mouse model of atopic dermatitis (AD),and to explore its potential mechanism.Methods Forty specific pathogen-free (SPF) female BABL/c mice were randomly divided into blank group (8 mice,receiving no treatment) and AD model group (32 mice).The mice in the model group were topically treated with 2,4-dinitrochlorobenzene (DNCB) in acetone/olive oil to establish the mouse model of AD.After modeling,8 mice in the blank group and 8 in the model group were sacrificed immediately.The other 24 mice in the model group were randomly and equally divided into 3 groups:model control group receiving no treatment,ESC group and ESC vehicle group topically treated with ESC and ESC vehicle respectively once every day on the back and aural region of the mice for 28 consecutive days.Changes in skin lesions were observed by naked eyes every day.A thickness tester was used to measure the thickness of skin lesions on the left ear before modeling,at completion of modeling and 12 hours after the final treatment.At 12 hours after the final treatment,the mice in the above 3 groups were sacrificed,and the eyeballs were removed for collecting blood.Then,the sera were isolated,and skin tissue specimens were obtained from the skin lesions on the back.These tissue sections were subjected to hematoxylin and eosin (HE) staining and toluidine blue staining for observing the infiltration of inflammatory cells in skin lesions.An immunohistochemical study was performed to determine the expression of aquaporin 3 (AQP3),Toll-like receptor 2 (TLR2) and TLR4,and enzyme-linked immunosorbent assay (ELISA) to detect the serum levels of IgE,interleukin-4 (IL-4) and interferon-/ (IFN-γ).Results After 28-day treatment,skin lesions were improved in the ESC group.Compared with the model control group,the ESC group showed a significantly lower clinical symptom score (1.50 ± 0.58 vs.2.50 ± 0.58,P ＜ 0.05),decreased lesional thickness on the left ear ([0.31 ± 0.01] mm vs.[0.33 ± 0.01] mm,P ＜ 0.05),and lower number of infiltrating mast cells per high-power field (15.18 ± 1.64 vs.28.94 ± 1.28,P ＜ 0.05).Immunohistochemical findings indicated that the ESC group showed significantly lower expression of AQP3,TLR2 and TLR4 compared with the model control group,and decreased AQP3 expression in the spinous layer.Compared with the model control group,the ESC group showed significantly lower total serum IgE and IL-4 levels,but higher IFN-γ levels (all P ＜ 0.05).Conclusion Topical ESC is effective for the treatment of skin lesions in mouse models of AD,likely by regulating serum levels of IgE,IL-4 and IFN-γ and affecting the expression of AQP3,TLR2 and TLR4.

Objective To analyze the effects of five proteins secreted by Chlamydia trachomatis on the phagocytosis of macrophages and dendritic cells derived from bone marrow cells of C3H/HeJ mice. Methods Glutathione S-transferase ( GST)-CT311, GST-GIgA, GST-cHtrA, GST-OmcBc and GST-Pgp3 proteins were prepared through an Escherichia coli prokaryotic expression system and purified by GST Mag-Beads. Chlamydia membrane protein GST-IncA was also prepared as a control. Proteins of interest were ob-tained by cleaving off GST-tag with PreScission protease. Macrophages (MΦ) and dendritic cells (DC) were prepared from bone marrow cells of C3H/HeJ mice and pretreated with either 100 μg/ml or 500 μg/ml of the above proteins. LPS was used as a control to testify the specificity of the proteins' functions. Four hours after pretreatment,fluorescent beads were added to culture media to evaluate the changes in phagocytosis with direct immunofluorescence assay. Results LPS and low concentration (100 μg/ml) of these proteins had no significant influence on the phagocytosis of DC and MΦ,while high concentration (500 μg/ml) of Pgp3, cHtrA and CT311 could significantly promote the phagocytosis of DC and MΦ. Conclusion Pgp3, cHtrA and CT311 can promote the in vitro phagocytosis of DC and MΦ,which may facilitate the in vivo dissemina-tion of Chlamyida trachomatis.