BACKGROUND:Cryopreservation can better ensure the integrity of the vascular structure.How to reduce its immunogenicity to improve rejection after transplantation has attracted more and more attention. OBJECTIVE:To review the research progress of freezing treatment to reduce vascular immunogenicity after allogeneic vascular transplantation in order to provide a reference for clinical research. METHODS:A systematic search of Chinese and English databases CNKI,WanFang and PubMed,as well as online websites Baidu and Google Scholar since the establishment of the database has published literature on reducing vascular immunogenicity after allogeneic vascular transplantation.Keywords were"cardiovascular disease,endothelial cells,cryopreservation,blood vessel transplantation or vascular graft,immunogenicity,immune rejection,allograft or allogeneic transplantation or allograft transplantation and cryoprotectant".A total of 68 articles were included according to the inclusion and exclusion criteria. RESULTS AND CONCLUSION:(1)The review found that the rejection of allogeneic vascular transplantation can be reduced by improving the existing freezing technology,which mainly involves the selection of freezing and thawing methods and cryoprotectants.(2)The existing research suggests that the freeze-drying method is superior to the low-temperature cryopreservation,but due to the limited conditions,it is still dominated by low-temperature cryopreservation.Among them,vitrification cryopreservation,slow rewarming and the use of stainless steel and even silver-containing materials are better than programmed cryopreservation and rapid rewarming.(3)The combined selection of permeable and non-permeable cryoprotectants can further reduce the occurrence of rejection while reducing their toxicity.

Surface Laplacian of the body surface potential (Laplacian ECG--LECG) is a new approach to resolve spatially distributed bioelectrical source. In this paper, we discussed an LECG sensor which integrated triple concentric ring electrodes and signal adjustor on a printed board. The LECG is measured directly by this sensor. The frequency, amplitude and phase of the power line interference were detected by a nonlinear adaptive filter so that interference was eliminated. The wavelet shrinking technique was used to eliminate the rest of random noise. And we got the high quality LECG signal. It laid the foundation for heart disease diagnosis.
Algorithms ; Body Surface Potential Mapping ; methods ; Electrocardiography ; methods ; Electrodes ; Humans ; Signal Processing, Computer-Assisted

EMG has been extensively used to study function of back muscle, which plays an important role in the objective assessment of occupational low back pain, yet the inherent large variability of EMG signals across subjects produced by reasons already known or unknown may mask true biological differences. Some useful parameters abstracted from 8-channel EMG signal in time-frequency domain are used or proposed and calculated in this paper to decrease this variability, when comparing the possible difference between low back pain patients and normal control group. The calculation of instantaneous median frequency is improved to decrease the interruption of background noise, and different power densities in EMG time-frequency spectrogram are observed in two tested groups.
Back ; Electromyography ; Fourier Analysis ; Humans ; Low Back Pain ; physiopathology ; Muscle, Skeletal ; physiopathology ; Spectrum Analysis

The part-time graduate student education is the important part of the graduate student system in Chinese medicine education.Developing and regulating the part-time graduate student education in Chinese medicine is helpful to the consummation of Chinese medicine graduate student system,the cultivation of the high lever talents in Chinese medicine,and keeping up with international graduate student educational system.