Objective To identify and analyze risk factors for acute renal failure (ARF) following lung transplantation and to develop a predictive model. Methods Data for this study were obtained from the United Network for Organ Sharing (UNOS) database, encompassing patients who underwent unilateral or bilateral lung transplantation between 2015 and 2022. We analyzed both preoperative and postoperative clinical characteristics of the patients. A combined approach utilizing random forest and least absolute shrinkage and selection operator (LASSO) regression was employed to identify key factors associated with the incidence of ARF post-transplantation, based on which a nomogram model was developed. The predictive performance of the constructed model was evaluated in both training and validation sets, using receiver operating characteristic (ROC) curves and area under the curve (AUC) metrics to verify and compare model effectiveness. Results A total of 15 110 lung transplantation patients were included in the study, consisting of6 041 males and 9 069 females, with a median age of 62.00 years (interquartile range: 54.00 to 67.00). The analysis revealed statistically significant differences between postoperative renal dialysis and non-dialysis patients regarding preoperative lung diagnosis, estimated glomerular filtration rate (eGFR), mechanical ventilation, preoperative ICU treatment, extracorporeal membrane oxygenation (ECMO) support, infections occurring within two weeks prior to transplantation, Karnofsky Performance Status (KPS) score, waitlist duration, double-lung transplantation, and ischemia time (P<0.05). Five key variables associated with ARF after lung transplantation were identified through random forest and LASSO regression: recipients’ eGFR, preoperative ICU treatment, ECMO support, bilateral lung transplantation, and ischemia time. A nomogram model was subsequently established. Model evaluation demonstrated that the constructed predictive model achieved high accuracy in both training and validation sets, with favorable AUC values, confirming its validity and reliability. Conclusion This study identifies common risk factors for ARF following lung transplantation and introduces an effective predictive model with potential clinical applications.

Background: SM-like (LSM) genes a family of RNA-binding proteins, are involved in mRNA regulation and can function as oncogenes by altering mRNA stability. However, their roles in B-cell progression and tumorigenesis remain poorly understood. Methods: We analyzed gene expression profiles and overall survival data of 123 patients with mantle cell lymphoma (MCL). The LSM index was developed to assess its potential as a prognostic marker of MCL survival. Results: Five of the eight LSM genes were identified as potential prognostic markers for survival in MCL, with particular emphasis on the LSM.index. The expression levels of these LSM genes demonstrated their potential utility as classifiers of MCL. The LSM.index-high group exhibited both poorer survival rates and lower RNA levels than did the overall transcript profile. Notably, LSM1 and LSM8 were overexpressed in the LSM.index-high group, with LSM1 showing 2.5-fold increase (p < 0.001) and LSM8 depicting 1.8-fold increase (p < 0.01) than those in the LSM.index-low group.Furthermore, elevated LSM gene expression was associated with increased cell division and RNA splicing pathway activity. Conclusions The LSM.index demonstrates potential as a prognostic marker for survival in patients with MCL. Elevated expression of LSM genes, particularly LSM1 and LSM8, may be linked to poor survival outcomes through their involvement in cell division and RNA splicing pathways. These findings suggest that LSM genes may contribute to the aggressive behavior of MCL and represent potential targets for therapeutic interventions.

Background: SM-like (LSM) genes a family of RNA-binding proteins, are involved in mRNA regulation and can function as oncogenes by altering mRNA stability. However, their roles in B-cell progression and tumorigenesis remain poorly understood. Methods: We analyzed gene expression profiles and overall survival data of 123 patients with mantle cell lymphoma (MCL). The LSM index was developed to assess its potential as a prognostic marker of MCL survival. Results: Five of the eight LSM genes were identified as potential prognostic markers for survival in MCL, with particular emphasis on the LSM.index. The expression levels of these LSM genes demonstrated their potential utility as classifiers of MCL. The LSM.index-high group exhibited both poorer survival rates and lower RNA levels than did the overall transcript profile. Notably, LSM1 and LSM8 were overexpressed in the LSM.index-high group, with LSM1 showing 2.5-fold increase (p < 0.001) and LSM8 depicting 1.8-fold increase (p < 0.01) than those in the LSM.index-low group.Furthermore, elevated LSM gene expression was associated with increased cell division and RNA splicing pathway activity. Conclusions The LSM.index demonstrates potential as a prognostic marker for survival in patients with MCL. Elevated expression of LSM genes, particularly LSM1 and LSM8, may be linked to poor survival outcomes through their involvement in cell division and RNA splicing pathways. These findings suggest that LSM genes may contribute to the aggressive behavior of MCL and represent potential targets for therapeutic interventions.

Background: SM-like (LSM) genes a family of RNA-binding proteins, are involved in mRNA regulation and can function as oncogenes by altering mRNA stability. However, their roles in B-cell progression and tumorigenesis remain poorly understood. Methods: We analyzed gene expression profiles and overall survival data of 123 patients with mantle cell lymphoma (MCL). The LSM index was developed to assess its potential as a prognostic marker of MCL survival. Results: Five of the eight LSM genes were identified as potential prognostic markers for survival in MCL, with particular emphasis on the LSM.index. The expression levels of these LSM genes demonstrated their potential utility as classifiers of MCL. The LSM.index-high group exhibited both poorer survival rates and lower RNA levels than did the overall transcript profile. Notably, LSM1 and LSM8 were overexpressed in the LSM.index-high group, with LSM1 showing 2.5-fold increase (p < 0.001) and LSM8 depicting 1.8-fold increase (p < 0.01) than those in the LSM.index-low group.Furthermore, elevated LSM gene expression was associated with increased cell division and RNA splicing pathway activity. Conclusions The LSM.index demonstrates potential as a prognostic marker for survival in patients with MCL. Elevated expression of LSM genes, particularly LSM1 and LSM8, may be linked to poor survival outcomes through their involvement in cell division and RNA splicing pathways. These findings suggest that LSM genes may contribute to the aggressive behavior of MCL and represent potential targets for therapeutic interventions.

Objective:To evaluate the post-marketing safety and immunogenicity of a 23-valent pneumococcal polysaccharide vaccine (PPV23).Methods:From September 2020 to June 2021, a clinical trial of single-dose PPV23 was conducted in people ≥3 years old in Centers for Disease Control and Prevention of Guizhou, Hunan and Fujian provinces. Blood samples were collects from the subjects before and 30 d after vaccination. ELISA was used to quantitatively detect IgG antibodies against capsular polysaccharides of 23 Streptococcus pneumoniae serotypes in serum samples. The adverse events (AEs) were monitored within 7 d after vaccination. Results:A total of 409 subjects were enrolled and included in safety analysis. Except for one with antibody level inversion, the other 408 participants were included in immunogenicity analysis. The levels of antibodies against the 23 Streptococcus pneumoniae serotypes were all increased after vaccination by an average of 4.24 folds. The two-fold growth rates of the antibodies ranged from 51.72% to 96.81% with a total two-fold growth rate of 78.59%. The overall rate of AEs was 27.14% (111/409). Local AEs were mainly pain, induration, redness and swollen. No serious adverse events related to vaccination occurred. Conclusions:This study preliminarily demonstrated the good immunogenicity and safety of PPV23 vaccine.

Objective:To explore the auxiliary 3D rapid prototyping technology of adult penis virtual surgery simulation on the basis of the force feedback, thus to improve the success rate of surgery, to reduce complications and to ensure the operation safety and reliability.Methods:Twenty cases of Inner Mongolia adult male (20-50 years old) were selected from January 2009 to December 2015 in the Department of Burns and Plastic Surgery, Inner Mongolia Medical University Affiliated Hospital. The two-dimensional CT data from the iliac crest edge to edge of penis continuously were input into the computer to reconstruct 3D penis and adjacent structure model using Magics Rapid-prototyping software. Under the Free Form system, digital virtual simulation reconstructive surgery was carried out based on force feedback device for adult penis.Results:Using Free Form system, virtual plastic and reconstructive surgery was carried on the adult penis, which could be used for penis optional operation, the three dimensional model for quantitative operation design to choose the best surgical approach. This technique provided a new technical means to avoid complications happened.Conclusions:Penis RP model can accurately reflect the complex anatomical characteristics and the internal space of the area adjacent to the relationship, based on force feedback, make the performer from the visual, auditory and tactile perception to simulate procedures for accurate adult penile reconstructive surgery and to improve clinical application value.

Objective:To construct a surface model of penis and adjacent structures with clear anatomical features by using the three-dimensional laser scanning technology and to explore the application value in the three-dimensional reconstruction of adult penis soft tissue.Methods:Sixty cases of healthy male subjects (25 to 28 years old) were chosen from January to December 2019 from Inner Mongolia region in Inner Mongolia Medical University Hospital; subjects were divided into 3D digital model measurement and manual measurement groups, each 30 cases. 3D digital model group was measured with 3D laser scanning for adult penis and adjacent to the structure of three-dimensional digital model of the related software on the penis morphological measurement. The results of the manual measurement group was compared with those of the 3D digital model group.Results:The model obtained by 3D laser scanning could rotate and scale smoothly, observe the penis and adjacent structure from any angle, conduct simulation operation, and complete measurement quickly and accurately, with no significant difference between the 3D digital model and manual measurement results.Conclusions:3D laser scanning technology is a simple, accurate and efficient method for soft tissue reconstruction and non-contact measurement of adult penis. The measurement results are completely reliable, and the measurement error conforms to the requirements of anthropometry, providing an important morphological data source for clinical penile plastic surgery.

Objective:To analyze the clinical effects of superselective angiography CT (CTA) three-dimensional reconstruction technique used in anterolateral thigh perforator flap (ALTPF) transplantation.Methods:According to the selection criteria, patients with limb soft tissue defects were treated with CT three-dimensional assisted surgery and ALTPF transplantation in the First People’s Hospital of Wuhu City from June 2016 to June 2017, which is CTA three-dimensional reconstruction technology group(CTA group). Patients with limb soft tissue defects were treated with handheld Doppler assisted surgery and ALTPF transplantation in the same hospital from April 2015 to April 2016, which is handheld Doppler group. The clinical efficacy, clinical indicators(the area of the flap, the operation time, the intraoperative blood loss, and the hospitalization time) and surgical complications of the two groups were analyzed 3 months after the operations. The data were statistically analyzed by SPSS22.0. t test was used for the comparison of measurement data between the two groups, and χ2 test was used for the comparison of rates. P<0.05 was considered statistically significant. Results:There were 36 patients in CTA group, which were 24 males and 12 females, aged (32.58±4.27) years old (23-54 years old). There were the same patients in handheld Doppler group, which were 23 males and 13 females, aged (32.14±5.34) years old (20-55 years old). There were no statistically significant differences between the two groups in gender, age, defect area, injury type and time from injury to admission ( P> 0.05). The excellent and good rate of patients in the CTA group was 91.67% (33/36), which was significantly higher than that in the handheld Doppler group (72.22 %, 26/36) ( χ2=4.600, P=0.032). The operation time[(237.57±50.41) min], hospitalization time[(21.40±4.43) d] and surgical bleeding[(247.86±62.04) ml] of the CTA group were significantly less than those of the handheld Doppler group[(281.56±70.96) min, (24.56±6.06) d, (305.62±106.12) ml]( t=3.032, 2.819, 2.526, P=0.003, 0.006, 0.014). The surgical complications rate (8.33%, 3/36) of CTA group was less than that of the handheld Doppler group(22.22%, 8/36), but there was no significant difference between the two groups ( χ2=2.683, P=0.102). Conclusions:Superselective angiography CT three-dimensional visualization reconstruction technique has a high rate of excellent anterior and lateral anterior perforating percutaneous flap, excellent clinical indicators and low complication rate.

Objective:To analyze the clinical effects of superselective angiography CT (CTA) three-dimensional reconstruction technique used in anterolateral thigh perforator flap (ALTPF) transplantation.Methods:According to the selection criteria, patients with limb soft tissue defects were treated with CT three-dimensional assisted surgery and ALTPF transplantation in the First People’s Hospital of Wuhu City from June 2016 to June 2017, which is CTA three-dimensional reconstruction technology group(CTA group). Patients with limb soft tissue defects were treated with handheld Doppler assisted surgery and ALTPF transplantation in the same hospital from April 2015 to April 2016, which is handheld Doppler group. The clinical efficacy, clinical indicators(the area of the flap, the operation time, the intraoperative blood loss, and the hospitalization time) and surgical complications of the two groups were analyzed 3 months after the operations. The data were statistically analyzed by SPSS22.0. t test was used for the comparison of measurement data between the two groups, and χ2 test was used for the comparison of rates. P<0.05 was considered statistically significant. Results:There were 36 patients in CTA group, which were 24 males and 12 females, aged (32.58±4.27) years old (23-54 years old). There were the same patients in handheld Doppler group, which were 23 males and 13 females, aged (32.14±5.34) years old (20-55 years old). There were no statistically significant differences between the two groups in gender, age, defect area, injury type and time from injury to admission ( P> 0.05). The excellent and good rate of patients in the CTA group was 91.67% (33/36), which was significantly higher than that in the handheld Doppler group (72.22 %, 26/36) ( χ2=4.600, P=0.032). The operation time[(237.57±50.41) min], hospitalization time[(21.40±4.43) d] and surgical bleeding[(247.86±62.04) ml] of the CTA group were significantly less than those of the handheld Doppler group[(281.56±70.96) min, (24.56±6.06) d, (305.62±106.12) ml]( t=3.032, 2.819, 2.526, P=0.003, 0.006, 0.014). The surgical complications rate (8.33%, 3/36) of CTA group was less than that of the handheld Doppler group(22.22%, 8/36), but there was no significant difference between the two groups ( χ2=2.683, P=0.102). Conclusions:Superselective angiography CT three-dimensional visualization reconstruction technique has a high rate of excellent anterior and lateral anterior perforating percutaneous flap, excellent clinical indicators and low complication rate.

The loss of endothelial connective integrity and endothelial barrier dysfunction can lead to increased vascular injury, which is related to the activation of endothelial inflammasomes. There are evidences that low concentrations of aspirin can effectively prevent cardiovascular diseases. We hypothesized that low-dose aspirin could ameliorate endothelial injury by inhibiting the activation of NLRP3 inflammasomes and ultimately prevent cardiovascular diseases. Microvascular endothelial cells were stimulated by lipopolysaccharide (2 μg/mL) and administrated by 0.1-2 mmol/L aspirin. The wild type mice were stimulated with LPS (100 μg/kg/day), and 1 h later treated with aspirin (12.5, 62.5, or 125 mg/kg/day) and dexamethasone (0.0182 mg/kg/day) for 7 days. Plasma and heart were harvested for measurement of ELISA and immunofluorescence analyses. We found that aspirin could inhibit NLRP3 inflammasome formation and activation in dose-dependent manner and has correlation between the NLRP3 inflammasome and the ROS/TXNIP pathway. We also found that low-concentration aspirin could inhibit the formation and activation of NLRP3 inflammasome and restore the expression of the endothelial tight junction protein zonula occludens-1/2 (ZO1/2). We assume that aspirin can ameliorate the endothelial layer dysfunction by suppressing the activation of NLRP3 inflammasome.