Splenogastric hygropyrexia syndrome, a common syndome in clinical practice, responds well to the therapy of traditional Chinese medicine (TCM). In view of the complexity of TCM syndrome formation, proceeding from different aspects such as Helicobacter pylori (Hp) infection, expression of inflammatory factors, mucosal protection and change in micro-ecosystem, the authors proposed that Hp infection in gastric mucosa, nuclear factor-kappa B activation, over-expression of heat shock protein 70, and "excessive evil, dyssplenism and confliction between healthy energy and evils" due to disequilibrium between Hp and lactobacillus acidophilus in gastric mucosa and coated tongue may be the important links in the formation of Hp related gastrosis with splenogastric hygropyrexia syndrome.

0.05). The PCNA and Ki-67 expression of blood stasis syndrome is more evident than of Qi stagnation (P

Objective To establish the animal model of damp-heat syndrome(DHS) and explore the expression of aquaporin(AQP) 3,4 gene in gastric mucosa.Methods Thirty SD rats were randomized into 3 groups and observed for 20 days.Group A(normal group,NG) was fed with routine method.Group B(Pi deficiency syndrome group,PDS) was fed alternatively with routine method and aqua of cassia angustifolia Vahl.Group C(DHS) was fed with fat,honey liquid and treated with damp-heat environment.The expression of AQP 3,4 gene in the 3 groups were determined by fluorescence quantitative polymerase chain reaction(FQ-PCR).Results The symptoms and signs in Group C were simiar to the clinical DHS.Expression of AQP3 gene in DHS was statistically significant higher than that in PDS and NG(P

【Objective】 To establish preliminary diagnostic criteria of spleen-stomach damp-heat syndrome (SDS) in chronic superficial gastritis (CSG) by disease-syndrome combined differentiation method and modern epidemiological method. 【Methods】 A clinical questionnaire of SDS in CSG was drafted out based on literatures study and experts' survey. Questionnaire investigation was performed in 146 cases of CSG, which consisted of 81 SDS cases and 65 nonSDS cases. Mter the investigation, discriminant analysis was adopted to set up the discriminant functional equations and to perform frequency analysis. According to the results, the diagnostic criteria and the simplified diagnostic criteria of SDS in CSG were established. 【Results】 The preliminary diagnostic criteria of SDS in CSG were as follows: (1) the major symptoms were characterized as yellow and greasy tongue fur, fullness or distension or pain in the stomach, loose stool and poor appetite; (2) the secondary symptoms were characterized as bitter taste and sticky mouth, chest depression, thirst but unwilling to drink, fatigue and nausea; (3) diagnosis could be confirmed by the existence of yellow and greasy tongue fur accompanied by either 2 of the other major symptoms, or by either 1 of the other major and 2 of the secondary symptoms, or by more than 3 of the secondary symptoms. The simplified diagnostic criteria were characterized the co-existence of yellow and greasy tongue fur and fullness or distension or pain in the stomach. 【Conclusion】 The preliminary diagnostic criteria of SDS in CSG are scientific, objective and practicable to some extent.

Achievements of the 30-year research work on Pi-Wei Doctrine were summarized. They are: (1) ratio of salivin activity and excretion rate of D-xylose in patients with spleen deficiency are different from those in the normal subjects;(2) the principle of syndrome differentiation and treatment is embodied in the methodology of herbal medicine pharmacology for complex prescription; (3) the above findings are further confirmed by the research in molecular and cellular level which indicates that TCM syndromes and pharmacological mechanisms of herbal medicines have their material basis and the regulatory effect of complex prescription on TCM syndromes is actually the pharmacological mechanism of herbal medicines. It is suggested that the research of herbal medicine should be based on the guidance of traditional Chinese medicinal theory and carried out in clinical practice.

【Objective】 To establish the quantified standard of spleen-stomach damp-heat syndrome(SSDHS) in chronic superficial gastritis(CSG).【Methods】Based on the epidemiological survey,the factors related to SSDHS and non-SSDHS were quantified and scored,and then the quantified standard was set up and used for the degree grading of SSDHS.【Results】The diagnostic threshold value of SSDHS in chronic superficial gastritis was 49 points.The results of retrospective examination showed that the sensitivity,specificity,accuracy and likelihood ratio of qualified SSDHS standard are 90.91%,90.57%,90.72% and 9.64 respectively.The graded criteria of SSDHS were as follows:49～57 points for the mild syndrome,58～65 points for the moderate syndrome,and over 65 points for the severe syndrome.【Conclusion】It is indicated that the qualified SSDHS standard in chronic superficial gastritis is practical for its preciseness and rationality,and is worth of further study.

0.05 ),and the gastric evacuation rate in SDS group was lower than Group C(P

[Objective] To observe the therapeutic effect of heat-clearing and dampness-eliminating therapy for chronic hepatitis B with damp-heat syndrome and its influence on soluble interleukin-2 receptor (SIL-2R). [Methods] Chronic hepatitis B patients with damp-heat syndrome were divided into two groups by incomplete randomization. Group A ( n = 31) was treated with heat-clearing and dampness-eliminating therapy and Group B (n = 17) with routine western medicine for protecting hepatic function. SIL-2R level in peripheral blood was observed before and after treatment. [Results] Serum levels of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TB) and SIL-2R in chronic hepatitis B patients were much higher than those in healthy volunteers before theatment and were decreased after treatment (P 0.05) and that of markedly effective rate was significant (P

[Objective] To investigate the pathological features, secretion of interleukin-8 (IL - 8), tumor necrosis factor alpha (TNF-?) and secretory immunoglobulin A (sIgA) and helicobacter pylori (HP) infection in gastric mucosa (GM) of chronic superficial gastritis with spleen-stomach and damp-heat syndrome (SSDHS) and to explore their correlations. [Methods] Fifty-five cases of chronic superficial gastritis included 35 cases of SSDHS (Group A) and 20 cases of spleen-deficiency syndrome (Group B). Fifteen asymptomatic volunteers served as normal control group (Group C). Pathological changes, HP infection rate, levels of IL - 8, TNF -? and sIgA in GM were observed. [Results] HP infection rate was higher and inflammatory change was more obvious in Group A than those in Group B and Group C (P 0.05). [Conclusion] Gastric mucosa of chronic superficial gastritis with SSDHS presents inflammatory changes, which is related with the increase of IL - 8 and TNF -? secretion and the decrease of sIgA, and HP infection may be one of the factors inducing and enhancing the pathological changes.

[Objective] To establish the animal model of spleen-stomach damp-heat syndrome (SDS). [Methods] Fifty SD male rats were randomized into 5 groups. Group A was fed with routine methods; group B was fed under the damp-heat environment; group C was fed with high fat and sugar diet and alternative feeding of fat and wine; group D was fed with a combined method of under damp-heat environment and giving high fat and sugar diet and wine; group E was fed by the method similar to group D and with Qingre Huashi Prescription at the same time. Fifteen days later, the symptoms and signs, and gastrin (GAS) and motilin (MTL) levels in the serum, plasma and gastrointestinal mucosa were observed to evaluate the models. [ Results] The symptoms and signs and pathological changes in Group D were similar to those of SDS. [Conclusion] The combined method of feeding under damp-heat environment and with high fat, sugar diet and wine supply a new data for the research of SDS.