Objective To investigate whether there is Blomia tropicalis breeding in the flour warehouse of food factory in a civil aviation airport,and,if there is,to observe the morphological structure of the mite. Methods The flour samples were col-lected from the flour warehouse of food factory in a civil aviation airport. The breeding mites were isolated from the samples by di-rect microscopy and made into specimens to observe with a light microscope. Results Female Blomia tropicalis was found in the collected flour samples. Under the microscope,the mite was nearly spherical,and it had no tergum and claw. Its apodemeⅠwas developed and connected in the midline. The reproductive hole was located between the foot Ⅲand IV level and was cov-ered with a pair of oblique genital folds. The footⅢandⅣhad no solenoids. The copulatory pouch was a long,slightly curved tube,extending from the end of the mite. Conclusions Blomia tropicalis has been found in the flour warehouse of a food factory in the civil aviation airport. The main distinguishing feature of this mite is that it has no tergum,its feet have no pectinate inferi-or tarsus scales or claw,the feetⅠgenu has only one solenidia,and feetⅢandⅣhave no solenidias.

Objective To investigate the breeding quantity and average breeding density of Gohieria fuscus in the grand dust flour of a flourmill and explore the prevention and control measures. Methods A certain amount of grand dust flour was collect-ed from a flourmill,and it was sieved. The powder was placed into the glass pan,and the mites were sought out under an optical microscope and made of specimens. The mites were identified on the basis of literature. Results In this survey,400 g samples were collected from 4 habitats. The average breeding density of mites was 3516/g. The mite was identified as Gohieria fuscus. Conclusions Gohieria fuscus is one of the widely distributed stored mites. It impacts the stored food and reduces the quality of food. In addition,the mite affects human health. Therefore,the preventive measures should be taken.

Objective To investigate the Suidasia nesbitti breeding status in the ground dust flour collected in Qiqihaer City. Methods Totally,16 aliquots of ground dust flour were respectively collected from a college canteen and 15 households in Qiq-ihaer City. Then 10 g dust flour was taken from individual sample for isolation of the mites that were made of slide specimen,and the mites were identified and classified under a microscope. Results Acaroid mites were found in 15 of the 16 aliquots of sam-ples(detection rate being 93.8%),and 7 species,belonging to 6 genera under 3 families,were identified. A total of 561 heads of mites were isolated from 160 g samples,with an average breeding density of 3.51 heads/g. The most breeding mite was associ-ated with S. nesbitti. Conclusions Various species of mites are breeding in the ground dust flour in Qiqihaer City,and S. nesbit-ti occurs the most. These findings indicate that effective measures should be taken to prevent and control the harm associated with acaroid mite contamination.

Objective To characterize the population dynamics and spatial distribution of Aleuroglyphus ovatus in the flour warehouse,so as to provide the basic evidence for improving the sampling guidelines that are essential for effective pest monitor?ing and management. Methods The samples from flour warehouses of four localities were collected,examined and counted for A. ovatus in every month in Wuhu City. The dispersion pattern target,Iwao m*/-x regression analysis and Taylor power method were used for analyzing the spatial distribution pattern of A. ovatus in the flour warehouses. Results The peaks of population dynamics of A. ovatus were discovered in July and September,respectively. The indexes of dispersion were as follows:I>0, CA>0,m*/-x >1. At the same time,the parameters in the equation of Iwao:m*=5.471+1.022-x (r=0.999)and Taylor:lgS2=0.697+1.111 lg-x (r=0.987)showed that the spatial distribution pattern of A. ovatus was assembled. Conclusion The peaks of population dynamics of A. ovatus in the flour warehouse are bimodal pattern ,and the spatial distribution pattern of A. ovatus is assembled.

Objective To investigate the species of Cheyletoidea mites breeding in the culture environment of Eupolyphaga sinensis and to observe the morphology of Eucheyletia reticulata Cunliffe. Methods The soil samples from an E. sinensis farm in northern Anhui were collected. The mites in the soil samples were separated directly under a microscope and the glass speci?mens were made to observe the morphological feature of the mites under a light microscope,then the mites species were identi?fied and classified based on the morphological characteristics. Results In the culture soil of E. sinensis,totally 7 kinds of Chey?letoidea mites were isolated,namely Eucheyletia reticulata Cunliffe,Cheyletus eruditus Schrank,Cheyletus malaccensis Oudemans,Cheyletus troussarti Oudemans,Cheyletus aveisor Rohdendorz,Acaropsis sollers Rohdendorz and Cheletomor?pha lepidopterorum Shaw. They belonged to genera Eucheyletia,Cheletomorpha,Acaropsis and Cheyletus of Cheyletidae Leach family. The Eucheyletia reticulata Cunliffe was firstly found in the culture environment of E. sinensis,and its gnathosoma was large,the pedipalpal femurs were expanding and there were two strips of comb hair and two smooth bristles on the pedipal?pal tarsus,and the back of the body was covered with two pieces of tergum,which were decorated with reticular pattern. The body and foot setae were fan?shape. Conclusions There are various of Cheyletoidea mites found in the breeding environment of E. sinensis. These mites are important species for pest control in the culture environments of E. sinensis. Related measures should be taken to prevent the excessive growth of Cheyletoidea mites,so as to avoid the adverse effects on the quality and quan?tity of E. sinensis.

Objective To observe the Tyrophagus palmarum and its hypopus in feed of Tenebrio molitor. Methods Feed samples were collected from T. molitor farms,and T. palmarum and its hypopus were separated from the samples by the vibra?tion sieve method and floating method. The glass specimens were prepared and T. palmarum and its hypopus were observed un?der an optical microscope. Results Under the light microscope,the skin of T. palmarum was smooth and bright,the color of legs and chelicerae was relatively dark,the length of dorsal seta d2 was 3?4 times longer than d1 and la;Theω1 in tarsus of legⅠ,Ⅱwas short rod,and its top was not tapered;the sucker plate in tarsus of legⅣlocated in the middle of the section. The hypopus was oblate,and there were two pairs of legs in front which extended to body. The rear end of hypopus became pointless and round,wrapping around a transparent and ossification skin. Conclusion Light microscopy shows the morphological charac?teristics of T. palmarum and its dormant body,providing the basis for identifying the hypopus.

Objective To investigate the prevalence of trematode Aspidogastrea in freshwater mussels in Anhui Province, China. Methods The freshwater mussels living in different water areas within Anhui territory were harvested and dissected from April to May,2015. Then the flukes were collected from the pericardial cavities of the clams into a petri dish containing small amount of saline. After rinsing,the flukes were transferred into a bottle containing 70%ethanol for following identification by staining of the specimens prepared as previous protocol. Results A total of 3 007 calms in 12 species,including Unio doug?lasiae(603),Acuticosta chinensis(90),Lamprotula caveat(150),Lamprotula leai(250),Lanceolaria grayana(47),An?odonta pacifica(60),Anodonta woodiana(350),Cuneopsis heudei(100),Solenaia oleivora(150),Cristaria plicata (567),Hyriopsis cumingii(550)and Arconaia lanceolata(90)were dissected,in which 1 467 mussels were infected with the trematode Aspidogastrea,with an infection rate of 48.79%(1 467/3 007). Totally,7 306 flukes belonging to 3 genera under the subclass of Aspidogastrea were isolated. These trematodes were genera of Aspidogaster,Lophotaspis and Cotylaspis. The in?fection density was from 1 to 128 capita for individual positive clam,with a mean infectiosity of 4.98(7306/1467)for each mus?sel. Conclusion The freshwater bivalves living in different water areas in Anhui Province are infected with the trematode Aspi?dogastrea. These flukes were identified as Aspidogaster sp. Lophotaspis sp. and Cotylaspis sp. belonging to subclass Aspidogas?tridae of family Aspidogastrea.

Objective To investigate the species of Acaroid mites breeding in the stored traditional Chinese animal medici?nal materials and the relationship between its community and habitats. Methods A total of 30 samples of traditional Chinese animal medicinal herbs were collected from Wuhu City,Anhui Province,China. The mites were isolated by the directly micro?scopic and floatation microscopic examinations,and then identified and counted under a light microscope. Results Acaroid mites was represented in 28 of the 30 samples,and the breeding rate accounted for as high as 93.3%(28/30). Totally,13 spe?cies of Acaroid mites were identified,which belonged to 4 families and 9 genera. The densities of Acaroid mites were top in 6 Chinese herbal medicines,such as corium erinacei,aspongopus,hirudo,pheretima aspergillum,Apostichopus and huechys. The diversity parameters of these six traditional Chinese animal medicinal herbs were calculated. The highest richness indexes were in aspongopus and hirudo,the highest diversity index was in hirudo,and the highest evenness index was in Apostichopus. Conclusions There are Acaroid mites breeding in parts of the traditional Chinese animal medicinal herbs stored in Wuhu. In the storage and processing of Chinese herbal medicines,we should pay attention to the prevention and control of mites.

Objective To investigate the epidemic situation of Metorchis orientalis in Wuhu City Anhui Province. Meth?ods Three lakes Fengming Longwo and Kui lakes were selected in Wuhu City and the poultries around the lakes and fresh?water fishes in the lakes were captured to test the infection of M. orientalis and the infection rates were calculated. Results The adult worms of M. orientalis were found in gall bladder and hepatic duct of domestic ducks with infection rate of 16.7%. The metacercariae of M. orientalis were discovered in the muscle of Pseudorasbora parva and Pseudogobio rivularis with infection rates of 7.13%and 3.38%respectively. Conclusion M. orientalis is endemic in Wuhu City which should be paid enough at?tention to the animal husbandry aquaculture and medicine.

Objective To evaluate the effects of curcumin on the expression of receptor for advanced glycation end-products (RAGE) in the spinal dorsal horn and dorsal root ganglion (DRG) of the rats with type 2 diabetic neuropathic pain (DNP).Methods Male Sprague-Dawley rats,weighing 160-180 g,were used in this study.Type 2 diabetes mellitus was induced by high-fat and high-sucrose diet for 8 weeks and intraperitoneal streptozotocin (STZ) 35 mg/kg and confirmed by fasting blood glucose level ≥ 16.7 mmol/L 3 days later.Type 2 DNPwas confirmed by the mechanical paw withdrawal threshold (MWT) and thermal paw withdrawal latency (TWL) measured on day 14 after STZ administration less than 80％ of the baseline value.Eighty-one rats with type 2 DNP were randomly divided into 3 groups (n =27 each) using a random number table:DNP group,DNP + curcumin group (DCur group),and DNP+ solvent group (group DSC).In DCur and DSC groups,curcumin 100 mg· kg-1 · d-1 and corn oil 4 ml · kg-1 · d-1 were injected intraperitonally,respectively,for 14 consecutive days starting from the day 14 after STZ administration.Another 27 normal male Sprague-Dawley rats served as control group (group C) and were fed with normal forage.MWT and TWL were measured before STZ injection,at day 14 after STZ injection,and on 3,7 and 14 days after curcumin injection.RAGE positive cells were determined by immuno-histochemistry and the expression of RAGE by Western blot in the spinal dorsal horn and DRG after MWT and TWL were measured on 3,7 and 14 days after curcumin injection.Results Compared with group C,MWT was significantly decreased and TWL was shortened at 14 days after STZ injection and each time point after curcumin injection,the rate of RAGE positive cells in the spinal dorsal horn and DRG was increased at each time point after curcumin injection,and the expression of RAGE was up-regulated in the spinal dorsal horn at each time point after curcumin injection and in the DRG at 7 and 14 days after curcumin injection in group DNP.Compared with group DNP,MWT was significantly increased and TWL was prolonged at each time point after curcumin injection,the rate of RAGE positive cells in the spinal dorsal horn and DRG was significantly decreased at 7 and 14 days after curcumin injection,and the expression of RAGE was down-regulated in the spinal dorsal horn at each time point after curcumin injection and in the DRG at 7 and 14 days after curcumin injection in group DCur,and no significant change was found in the parameters mentioned above in group DSC.Conclusion The mechanism by which curcumin attenuates DNP may be related to inhibition of up-regulated expression of RAGE in the spinal dorsal horn and DRG of rats with type 2 diabetes mellitus.