This study examined the effects of Healing Beats on anxiety, stress, and well-being in hemodialysis patients, exploring its potential as an intervention to improve these outcomes. Methods: A randomized controlled trial included 65 hemodialysis patients with chronic kidney disease, divided into the Healing Beats group (HBG, 21 participants), preferred music group (PMG, 22 participants), and control group (CG, 22 participants). The HBG was exposed to music synchronized with their heartbeats during each dialysis session, while the PMG listened to music of their own selection. The CG received no music intervention. State anxiety, subjective stress, stress indices, and well-being were assessed before and after the experimental intervention. Results: The application of Healing Beats had a positive effect on the stress index of hemodialysis patients, with statistically significant differences between the three groups (F = 4.60, p < .001). However, no significant differences were observed in terms of anxiety and well-being across the groups after the intervention. Conclusion: Although hemodialysis patients have notably high stress levels, the application of Healing Beats and preferred music demonstrated limitations in alleviating anxiety and enhancing well-being. Although Healing Beats proved effective in reducing the stress index, it did not yield significant improvements in subjective stress, anxiety, or well-being. Further research is required to confirm these findings and explore potential refinements in the intervention.

This study examined the effects of Healing Beats on anxiety, stress, and well-being in hemodialysis patients, exploring its potential as an intervention to improve these outcomes. Methods: A randomized controlled trial included 65 hemodialysis patients with chronic kidney disease, divided into the Healing Beats group (HBG, 21 participants), preferred music group (PMG, 22 participants), and control group (CG, 22 participants). The HBG was exposed to music synchronized with their heartbeats during each dialysis session, while the PMG listened to music of their own selection. The CG received no music intervention. State anxiety, subjective stress, stress indices, and well-being were assessed before and after the experimental intervention. Results: The application of Healing Beats had a positive effect on the stress index of hemodialysis patients, with statistically significant differences between the three groups (F = 4.60, p < .001). However, no significant differences were observed in terms of anxiety and well-being across the groups after the intervention. Conclusion: Although hemodialysis patients have notably high stress levels, the application of Healing Beats and preferred music demonstrated limitations in alleviating anxiety and enhancing well-being. Although Healing Beats proved effective in reducing the stress index, it did not yield significant improvements in subjective stress, anxiety, or well-being. Further research is required to confirm these findings and explore potential refinements in the intervention.

This study examined the effects of Healing Beats on anxiety, stress, and well-being in hemodialysis patients, exploring its potential as an intervention to improve these outcomes. Methods: A randomized controlled trial included 65 hemodialysis patients with chronic kidney disease, divided into the Healing Beats group (HBG, 21 participants), preferred music group (PMG, 22 participants), and control group (CG, 22 participants). The HBG was exposed to music synchronized with their heartbeats during each dialysis session, while the PMG listened to music of their own selection. The CG received no music intervention. State anxiety, subjective stress, stress indices, and well-being were assessed before and after the experimental intervention. Results: The application of Healing Beats had a positive effect on the stress index of hemodialysis patients, with statistically significant differences between the three groups (F = 4.60, p < .001). However, no significant differences were observed in terms of anxiety and well-being across the groups after the intervention. Conclusion: Although hemodialysis patients have notably high stress levels, the application of Healing Beats and preferred music demonstrated limitations in alleviating anxiety and enhancing well-being. Although Healing Beats proved effective in reducing the stress index, it did not yield significant improvements in subjective stress, anxiety, or well-being. Further research is required to confirm these findings and explore potential refinements in the intervention.

This study examined the effects of Healing Beats on anxiety, stress, and well-being in hemodialysis patients, exploring its potential as an intervention to improve these outcomes. Methods: A randomized controlled trial included 65 hemodialysis patients with chronic kidney disease, divided into the Healing Beats group (HBG, 21 participants), preferred music group (PMG, 22 participants), and control group (CG, 22 participants). The HBG was exposed to music synchronized with their heartbeats during each dialysis session, while the PMG listened to music of their own selection. The CG received no music intervention. State anxiety, subjective stress, stress indices, and well-being were assessed before and after the experimental intervention. Results: The application of Healing Beats had a positive effect on the stress index of hemodialysis patients, with statistically significant differences between the three groups (F = 4.60, p < .001). However, no significant differences were observed in terms of anxiety and well-being across the groups after the intervention. Conclusion: Although hemodialysis patients have notably high stress levels, the application of Healing Beats and preferred music demonstrated limitations in alleviating anxiety and enhancing well-being. Although Healing Beats proved effective in reducing the stress index, it did not yield significant improvements in subjective stress, anxiety, or well-being. Further research is required to confirm these findings and explore potential refinements in the intervention.

Background: Mammalian orthoreovirus type 3 (MRV3), which is responsible for gastroenteritis in many mammalian species including pigs, has been isolated from piglets with severe diarrhea. However, the use of pig-derived cells as an infection model for swineMRV3 has rarely been studied. Objectives: This study aims to establish porcine intestinal organoids (PIOs) and examine their susceptibility as an in vitro model for intestinal MRV3 infection. Methods: PIOs were isolated and established from the jejunum of a miniature pig.Established PIOs were characterized using polymerase chain reaction (PCR) and immunofluorescence assays (IFAs) to confirm the expression of small intestine-specific genes and proteins, such as Lgr5, LYZI, Mucin-2, ChgA, and Villin. The monolayered PIOs and threedimensional (3D) PIOs, obtained through their distribution to expose the apical surface, were infected with MRV3 for 2 h, washed with Dulbecco’s phosphate-buffered saline, and observed. Viral infection was confirmed using PCR and IFA. We performed quantitative realtime reverse transcription-PCR to assess changes in viral copy numbers and gene expressions linked to intestinal epithelial genes and antiviral activity. Results: The established PIOs have molecular characteristics of intestinal organoids. Infected PIOs showed delayed proliferation with disruption of structures. In addition, infection with MRV3 altered the gene expression linked to intestinal epithelial cells and antiviral activity, and these effects were observed in both 2D and 3D models. Furthermore, viral copy numbers in the supernatant of both models increased in a time-dependent manner. Conclusions We suggest that PIOs can be an in vitro model to study the infection mechanism of MRV3 in detail, facilitating pharmaceutical development.

The seed borne disease such as bakanae is difficult to control. Crop yield loss caused by bakanae depending on the regions and varieties grown, ranging from 3.0% to 95.4%.Bakanae is an important disease of rice worldwide and the pathogen was identified as Fusarium fujikuroi Nirenberg (teleomorph: Gibberella fujikuroi Sawada). Currently, four Fusaria (F. fujikuroi, F. proliferatum, F. verticillioides and F. andiyazi) belonging to F. fujikuroi species complex are generally known as the pathogens of bakanae. The infection occurs through both seed and soil-borne transmission. When infection occurs during the heading stage, rice seeds become contaminated. Molecular detection of pathogens of bakanae is important because identification based on morphological and biological characters could lead to incorrect species designation and time-consuming. Seed disinfection has been studied for a long time in Korea for the management of the bakanae disease of rice. As seed disinfectants have been studied to control bakanae, resistance studies to chemicals have been also conducted. Presently biological control and resistant varieties are not widely used. The detection of this pathogen is critical for seed certification and for preventing field infections. In South Korea, bakanae is designated as a regulated pathogen. To provide highly qualified rice seeds to farms, Korea Seed & Variety Service (KSVS) has been producing and distributing certified rice seeds for producing healthy rice in fields. Therefore, the objective of the study is to summarize the recent progress in molecular identification, fungicide resistance, and the management strategy of bakanae.

Objective: The air pollution on pregnancy outcome (APPO) study is a prospective hospital-based cohort study designed to investigate the maternal and fetal effects of a particulate matter with an aerodynamic below 10 μm (PM10) and PM2.5 (below 2.5 μm) exposure. This study aims to analyze a relationship between particulate matter and adverse pregnancy outcomes and to find related biomarkers and develop management guidelines. Methods: About 1,200 pregnant women are recruited for 3 years (from January 2021 to December 2023) from seven university hospitals to investigate the effects of particulate matter on pregnancy complications and adverse pregnancy outcomes. We collect biological samples by 5 mL of maternal venous blood and 15 mL of urine in each trimester of pregnancy, and 5 mL of umbilical cord blood and 2×2×2 cm of placental tissue are collected after delivery. In addition, by applying PM10 and PM2.5 concentration values and time-activity patterns from the time weighted average model, the individual predicted exposure of air pollution for the pregnant women are obtained. Results: The average exposure of PM10 and PM2.5 of the participants in the entire period of pregnancy, was exceeded the World Health Organization air quality guidelines (an annual level, PM10 >15 μg/m3, PM2.5 >5 μg/m3). Moreover, it was revealed that the PM concentration was increasing toward the 3rd trimester of pregnancy. Conclusion The APPO study will be able to identify the degree of exposure to air pollution in pregnant women and use it as basic data for estimating individual exposure to particulate matter. And the results of the APPO study will facilitate in the development of health management for pregnant women against air pollution.

Background: As the number of large-scale studies involving multiple organizations producing data has steadily increased, an integrated system for a common interoperable format is needed. In response to the coronavirus disease 2019 (COVID-19) pandemic, a number of global efforts are underway to develop vaccines and therapeutics. We are therefore observing an explosion in the proliferation of COVID-19 data, and interoperability is highly requested in multiple institutions participating simultaneously in COVID-19 pandemic research. Results: In this study, a laboratory information management system (LIMS) approach has been adopted to systemically manage various COVID-19 non-clinical trial data, including mortality, clinical signs, body weight, body temperature, organ weights, viral titer (viral replication and viral RNA), and multiorgan histopathology, from multiple institutions based on a web interface. The main aim of the implemented system is to integrate, standardize, and organize data collected from laboratories in multiple institutes for COVID-19 non-clinical efficacy testings. Six animal biosafety level 3 institutions proved the feasibility of our system. Substantial benefits were shown by maximizing collaborative high-quality non-clinical research. Conclusions This LIMS platform can be used for future outbreaks, leading to accelerated medical product development through the systematic management of extensive data from non-clinical animal studies.

Purpose: Next-generation sequencing (NGS)-based tumor panel testing has been reimbursed by the Korean government since 2017. We evaluated the use of NGS-based tumor panel testing in real-world clinical practice, focusing on molecular profiling (MP)-guided breast cancer treatment. Methods: A total of 137 breast cancer patients underwent NGS panel testing between December 2017 and July 2020 at Seoul National University Bundang Hospital (SNUBH).Samples from patients were profiled using an in-house SNUBH pan-cancer panel. Sixty-four patients were profiled on SNUBH Pan_Cancer v1.0, targeting 89 genes, while 73 patients were profiled on SNUBH Pan_Cancer v2.0, targeting 546 genes. Results: Breast cancer subtypes included hormone receptor+/human epidermal growth factor receptor 2 (HER2)− (n = 87), triple-negative (n = 44), and HER2+ (n = 6). Most patients had locally advanced or metastatic cancers (92%). Approximately 92% (126/137) of the patients had significant genomic alterations (tiers I and II), and 62% (85/137) had targetable genomic alterations. The most common targetable genomic alterations were PIK3CA (39%) and ESR1 mutations (9%), followed by ERBB2 (7%), PTEN (7%), BRCA2 (6%), and BRCA1 mutations (4%). Of the 81 patients with locally advanced/metastatic breast cancer with targetable genomic alterations, 6 (7.4%) received MP-guided treatments, including PARP inhibitor (n = 4), ERBB2-directed therapy (n = 1), and PI3K inhibitor (n = 1). Among these 6 patients, 4 participated in clinical trials, 1 underwent treatment at their own expense, and 1 received drugs through an expanded access program. The remaining 66 patients (81%) with targetable genomic alteration did not receive MP-guided treatment due to lack of matched drugs and/or clinical trials, poor performance status, and/or financial burden. Conclusion NGS panel testing allowed MP-guided treatment in only 4.7% (6/127) of patients with advanced breast cancer in a real-world setting. The availability of matched drugs is critical for the realistic implementation of personalized treatment.

BACKGROUND: Decreased heart rate variability (HRV) is a predictor of autonomic system dysfunction, and is considered as a potential mechanism of increased risk of cardiovascular disease (CVD) induced by exposure to particulate matter less than 2.5 μm in diameter (PM METHODS: An updated systematic review and meta-analysis of panel studies till November 1, 2019 was conducted to evaluate the acute effect of exposure to ambient PM RESULTS: A total of 33 panel studies were included in our meta-analysis, with 16 studies conducted in North America, 12 studies in Asia, and 5 studies in Europe. The pooled results showed a 10 μg/m CONCLUSION Short-term exposure to PM
Adult ; Aged ; Aged, 80 and over ; Air Pollutants/analysis* ; Air Pollution/analysis* ; Female ; Heart Rate/drug effects* ; Humans ; Male ; Middle Aged ; Particulate Matter/analysis* ; Young Adult