【Objective】 To explore the feasibility of blood transfusion compatibility testing for multiple myeloma(MM) patients treated with anti-CD38 monoclonal antibody daratumumab (DARA) after DARA-Fab fragment blocking, and to evaluate the transfusion efficacy by comparing with dithiothreitol(DTT) method. 【Methods】 After DARA was prepared into DARA-Fab fragments using PierceFab preparation kit, the neutralization effects of different volumes (5, 10, 15, 30 μL) on screening cells and panel cells were confirmed. DARA-Fab fragments and screening cells with specific antigens and corresponding monoclonal antibody reagents were used as the experimental group and the control group with the same volume of saline for incubating and centrifugin.Twenty MM patients treated with DARA were selected for cross-matching with DARA-Fab and DTT respectively, and the laboratory indexes before and after transfusion were statistically analyzed, and the two blood matching methods were compared. 【Results】 After incubating and centrifuging, the results of DARA-Fab fragments(15, 30 μL) with screening cells and serum mixed with DARA were negative, while those of DARA-Fab(5, 10 μL) were positive. 15μL DARA-Fab treated antibody identification cells (2, 3, 4, 5, 7, 9, 11) were negative, antibody identification cells (1, 6, 8, 10, 12) were negative after 30 μL DARA-Fab fragments treatment; the results of MNS, Duffy, Kidd, Kell, Lewis, Rh blood group system of the experimental group were consistent with those of the control group; the hemoglobin (Hb) (g/L) of 20 patients after infusion of RBC (73.90±1.90) was significantly higher than that before transfusion (63.60±1.58), P<0.01. There was no significant difference in total bilirubin(TBil)(μmol/L)(16.25±3.54 vs 17.87±3.57), direct bilirubin(DBIL)(μmol/L)(6.31±2.32 vs 7.10±2.80)and indirect bilirubin(I-Bil)(9.94±1.38 vs 10.77±1.22) before and after infusion(P>0.05).And no statistical difference was noticed in Hb (10.75±1.04 vs 10.30±0.98), TBil (3.31±1.47 vs 3.31±0.55), DBIL(2.76±1.24 vs 2.60±0.83), and I-Bil(1.97±0.40 vs 2.82±0.53) between the DTT treatment method and the DARA Fab fragment treatment before and after transfusion(P>0.05). 【Conclusion】 DARA-Fab can remove the interference of RBC on cross matching by blocking CD38 antigen. This method has no effect on the antigens of common RBC blood group systems, and shows significant blood transfusion efficacy as that of DTT method.

Objective:To explore effects of somatosensory games combined with high frequency repetitive transcranial magnetic stimulation on cognitive function rehabilitation in patients with mild cognitive impairment after stroke.Methods:Using the convenience sampling method, a total of 64 patients with post stroke cognitive impairment (PSCI) admitted to Rehabilitation Department of the First Affiliated Hospital of Wenzhou Medical University from January to June 2021 were selected as the research objects. According to the order of admission and the 1∶1 matching principle, they were randomly divided into the control group and the observation group, with 32 cases in each group. The patients in the control group received high-frequency repetitive transcranial magnetic stimulation and conventional cognitive rehabilitation training, while the observation group received somatosensory game training on the basis of the control group. Scores of National Institute of Health Stroke Scale (NIHSS) , Montreal Cognitive Assessment (MoCA) , Mini-mental State Examination (MMSE) and Modified Barthel Index (MBI) were compared between the two groups before and after the intervention.Results:After 20 days of intervention, the NIHSS score of the observation group was lower than that of the control group, and the difference was statistically significant ( P<0.05) . The scores of MoCA, MMSE and MBI in the observation group were higher than those in the control group, and the differences were statistically significant ( P<0.05) . The compliance rate of rehabilitation therapy in the observation group was higher than that in the control group, and the difference between the two groups was statistically significant (χ 2=4.267, P=0.039) . Conclusions:Somatosensory games combined with high-frequency repetitive transcranial magnetic stimulation can improve the cognitive ability of patients with PSCI, improve the compliance rate of patients with rehabilitation exercises and help patients recover.