1.The design and outputs of the pilot implementation of the “Enhancing skills in screening and assessment for physicians and rehabilitation practitioners level 2 course”, Philippines, 2014
Carl Abelardo T. Antonio ; Kristine Joy L. Tomanan ; Eleanor C. Castillo ; Jonathan P. Guevarra ; Lolita L. Cavinta ; Mariano S. Hembra ; Ma. Lourdes Reyes-Sare ; Clara H. Fuderanan ; Salvador Benjamin D. Vista
Acta Medica Philippina 2022;56(5):75-81
Background and Objectives: The Philippine Department of Health (DOH) is mandated by law to, among others, develop capacities and accredit physicians and rehabilitation practitioners across the country on the assessment and management of drug dependence. This paper describes the design and presents the outputs of an advanced course on screening and assessment of drug dependence developed by DOH in partnership with the College of Public Health of the University of the Philippines Manila, Philippine College of Addiction Medicine, and the Group for Addiction Psychiatry of the Philippines.
Methodology: Review, abstraction and synthesis of data from training-related documents and records for the training activities implemented in 2014.
Results: The Level 2a course is a five-day program that focuses on enhancing the skills of physicians and rehabilitation practitioners on the screening and assessment of drug dependence using team-based and practical learning approaches, and builds on learnings from the basic accreditation course. A total of 36 participants from ten Drug Abuse Treatment and Rehabilitation Centers (DATRCs) in nine regions completed the pilot implementation of the course in 2014. In general, the overall participant feedback on the training was mainly favorable based on data from 47% of participants who agreed or strongly agreed to statements on the relevance and attainment of the course aims (mean rating of 1.10±0.31, 1 = Strongly agree, 5 = Strongly disagree), and the appropriateness of its content (1.24±0.43) and design (1.18±0.39). A paired-samples t-test comparing scores for 44% of participants showed that there was a highly statistically significant difference in the pre-test (54%±13%) and post-test scores (69%±10%); t(16)=6.4240, p <0.0001.
Conclusion: Development and design of capability-building initiatives in the field of drug rehabilitation will necessitate alignment with practice standards, grounding in the real-world setting in which professionals work, and orientation towards practical learning.
Education ; Interprofessional Relations ; Substance-Related Disorders ; Substance Abuse Treatment Centers ; Physicians ; Nurses ; Psychology ; Social Workers
2.Does Adopting a Multidisciplinary Approach in the Management of Acute Hip Fractures in Orthopedic Geriatric Patients Lead to Better Outcomes? A Preliminary Report of the University of the Philippines - Philippine General Hospital (UP-PGH) Orthogeriat
Paula Veronica S.J. Reyes ; Irewin A. Tabun ; Mark Anthony S. Sandoval ; Antonio Alan S. Mangubat ; Dorothy Dy Ching Bing-Agsaoa
Acta Medica Philippina 2021;55(3):308-314
OBJECTIVES: This study is a retrospective cohort that aims to describe the profile of orthopedic geriatric patients aged 60 years old and above with acute hip fractures occurring within one month of admission in the Philippine General Hospital managed by a multidisciplinary team approach.
METHODS: Data collection from the database was done to gather information regarding the patient profile, time to surgery, causes for delay in consultation, length of hospital stay, and follow-up rate, since this model was implemented.
RESULTS: Overall, we saw a decrease in the time from admission to surgery and a decrease in the length of hospital stay, when compared to previous data. Follow-up rates and compliance to maintenance medication are at 96% and 72% respectively.
CONCLUSION: The implementation of a multidisciplinary approach and fracture liaison service presents us with favorable results in addressing hip fractures and osteoporosis.
Orthopedics
;
Osteoporotic Fractures
;
Geriatrics
;
Interdisciplinary Research
;
Osteoporosis
;
Orthopedic Procedures
3.Management of isolated mandibular body fractures in adults
José ; Florencio F. Lapeñ ; a, Jr. ; Joselito F. David ; Ann Nuelli B. Acluba - Pauig ; Jehan Grace B. Maglaya ; Enrico Micael G. Donato ; Francis V. Roasa ; Philip B. Fullante ; Jose Rico A. Antonio ; Ryan Neil C. Adan ; Arsenio L. Pascual III ; Jennifer M. de Silva- Leonardo ; Mark Anthony T. Gomez ; Isaac Cesar S. De Guzman ; Veronica Jane B. Yanga ; Irlan C. Altura ; Dann Joel C. Caro ; Karen Mae A. Ty ; Elmo . R. Lago Jr ; Joy Celyn G. Ignacio ; Antonio Mario L. de Castro ; Policarpio B. Joves Jr. ; Alejandro V. Pineda Jr. ; Edgardo Jose B. Tan ; Tita Y. Cruz ; Eliezer B. Blanes ; Mario E. Esquillo ; Emily Rose M. Dizon ; Joman Q. Laxamana ; Fernando T. Aninang ; Ma. Carmela Cecilia G. Lapeñ ; a
Philippine Journal of Otolaryngology Head and Neck Surgery 2021;36(Supplements):1-43
Objective:
The mandible is the most common fractured craniofacial bone of all craniofacial fractures in the Philippines, with the mandibular body as the most involved segment of all mandibular fractures. To the best of our knowledge, there are no existing guidelines for the diagnosis and management of mandibular body fractures in particular. General guidelines include the American Academy of Otolaryngology – Head and Neck Surgery Foundation (AAOHNSF) Resident Manual of Trauma to the Face, Head, and Neck chapter on Mandibular Trauma, the American Association of Oral and Maxillofacial Surgeons (AAOMS) Clinical Practice Guidelines for Oral and Maxillofacial Surgery section on the Mandibular Angle, Body, and Ramus, and a 2013 Cochrane Systematic Review on interventions for the management of mandibular fractures. On the other hand, a very specific Clinical Practice Guideline on the Management of Unilateral Condylar Fracture of the Mandible was published by the Ministry of Health Malaysia in 2005. Addressing the prevalence of mandibular body fractures, and dearth of specific guidelines for its diagnosis and management, this clinical practice guideline focuses on the management of isolated mandibular body fractures in adults.
Purpose:
This guideline is meant for all clinicians (otolaryngologists – head and neck surgeons, as well as primary care and specialist physicians, nurses and nurse practitioners, midwives and community health workers, dentists, and emergency first-responders) who may provide care to adults aged 18 years and above that may present with an acute history and physical and/or laboratory examination findings that may lead to a diagnosis of isolated mandibular body fracture and its subsequent medical and surgical management, including health promotion and disease prevention. It is applicable in any setting (including urban and rural primary-care, community centers, treatment units, hospital emergency rooms, operating rooms) in which adults with isolated mandibular body fractures would be identified, diagnosed, or managed. Outcomes are functional resolution of isolated mandibular body fractures; achieving premorbid form; avoiding use of context-inappropriate diagnostics and therapeutics; minimizing use of ineffective interventions; avoiding co-morbid infections, conditions, complications and adverse events; minimizing cost; maximizing health-related quality of life of individuals with isolated mandibular body fracture; increasing patient satisfaction; and preventing recurrence in patients and occurrence in others.
Action Statements
The guideline development group made strong recommendationsfor the following key action statements: (6) pain management- clinicians should routinely evaluate pain in patients with isolated mandibular body fractures using a numerical rating scale (NRS) or visual analog scale (VAS); analgesics should be routinely offered to patients with a numerical rating pain scale score or VAS of at least 4/10 (paracetamol and a mild opioid with or without an adjuvant analgesic) until the numerical rating pain scale score or VAS is 3/10 at most; (7) antibiotics- prophylactic antibiotics should be given to adult patients with isolated mandibular body fractures with concomitant mucosal or skin opening with or without direct visualization of bone fragments; penicillin is the drug of choice while clindamycin may be used as an alternative; and (14) prevention- clinicians should advocate for compliance with road traffic safety laws (speed limit, anti-drunk driving, seatbelt and helmet use) for the prevention of motor vehicle, cycling and pedestrian accidents and maxillofacial injuries.The guideline development group made recommendations for the following key action statements: (1) history, clinical presentation, and diagnosis - clinicians should consider a presumptive diagnosis of mandibular fracture in adults presenting with a history of traumatic injury to the jaw plus a positive tongue blade test, and any of the following: malocclusion, trismus, tenderness on jaw closure and broken tooth; (2) panoramic x-ray - clinicians may request for panoramic x-ray as the initial imaging tool in evaluating patients with a presumptive clinical diagnosis; (3) radiographs - where panoramic radiography is not available, clinicians may recommend plain mandibular radiography; (4) computed tomography - if available, non-contrast facial CT Scan may be obtained; (5) immobilization - fractures should be temporarily immobilized/splinted with a figure-of-eight bandage until definitive surgical management can be performed or while initiating transport during emergency situations; (8) anesthesia - nasotracheal intubation is the preferred route of anesthesia; in the presence of contraindications, submental intubation or tracheostomy may be performed; (9) observation - with a soft diet may serve as management for favorable isolated nondisplaced and nonmobile mandibular body fractures with unchanged pre - traumatic occlusion; (10) closed reduction - with immobilization by maxillomandibular fixation for 4-6 weeks may be considered for minimally displaced favorable isolated mandibular body fractures with stable dentition, good nutrition and willingness to comply with post-procedure care that may affect oral hygiene, diet modifications, appearance, oral health and functional concerns (eating, swallowing and speech); (11) open reduction with transosseous wiring - with MMF is an option for isolated displaced unfavorable and unstable mandibular body fracture patients who cannot afford or avail of titanium plates; (12) open reduction with titanium plates - ORIF using titanium plates and screws should be performed in isolated displaced unfavorable and unstable mandibular body fracture; (13) maxillomandibular fixation - intraoperative MMF may not be routinely needed prior to reduction and internal fixation; and (15) promotion - clinicians should play a positive role in the prevention of interpersonal and collective violence as well as the settings in which violence occurs in order to avoid injuries in general and mandibular fractures in particular.
Mandibular Fractures
;
Jaw Fractures
;
Classification
;
History
;
Diagnosis
;
Diagnostic Imaging
;
Therapeutics
;
Diet Therapy
;
Drug Therapy
;
Rehabilitation
;
General Surgery
4.Implementation of the training program on retooling of personnel of the center for Health Development Calabarzon, Philippines
Carl Abelardo T. Antonio ; Eleanor C. Castillo ; Carmelita C. Canila ; Richard S. Javier ; Racel G. Carreon ; Jennifer Christina T. Tiu ; Ma. Sophia Graciela L. Reyes ; Ernesto R. Gregorio Jr.
Acta Medica Philippina 2021;55(7):781-787
Background:
The College of Public Health, University of the Philippines Manila (CPH-UPM) was engaged by the Center for Health Development Calabarzon (CHD 4A) to design, implement and manage the retooling of their personnel following implementation of Executive Order No. 336 on the rationalization of the Philippine government’s executive branch.
Objective:
To describe the training design and present outputs of the training modules designed for the CHD 4A staff.
Methods:
We reviewed the project documentation, which included the inception report, minutes of meetings, training modules, and post-training reports. Abstracted information was validated through internal discussion by a core group, which had representatives from the two organizations involved from project inception to close-out.
Results:
The design, development and implementation of the training were the product of collaborative efforts between CHD 4A and the technical team from the College of Public Health, University of the Philippines Manila. Technical staff of CHD 4A were trained in the competency areas in which gaps between the expected and perceived level of performance across all salary grades were highest: results orientation, planning and organizing, technical expertise, quality service focus, coordination and networking, and managing change. Nine training courses were implemented from May to July 2016, which was attended by 230 participants. All training modules were highly rated by participants (range: 3.60 to 3.85) based on a four-point scale, with 4 as the highest rating and 1 as the lowest. Comparison of pre- and post-tests for the modules on coordination and networking, and managing change showed a statistically significant increase in scores at the conclusion of their respective sessions.
Conclusion
The design of a training program for an organization’s personnel must be tailor-fit to and answer the needs of its employees. Trainers must be willing to make dynamic changes and adapt to immediate feedback from participants. The implementing party and organization itself must both ensure thorough evaluation of the effects of the training to achieve the organization’s long-term goals.
Staff Development
;
Teaching
;
Education
;
Workforce
5.Recovery in schizophrenia: Perspectives from psychiatrists in the Philippines.
Michael P. Sionzon ; Chelseah Denise H. Torres ; Carl Abelardo T. Antonio ; Carlo Eugenio V. Banaag ; Ma. Lourdes Rosanna E. de Guzman ; Monina Garduñ ; o-Cruz ; Mariano S. Hembra ; Benita Sta. Ana-Ponio ; Edgardo Juan L. Tolentino, Jr. ; Jessa Joy C. Malipot ; Dorothy Jean N. Ortega ; Ma. Sophia Graciela L. Reyes ; Angelica Beth T. Sia ; Charlene J. Tinaja ; Patrick Joseph Aldea ; Erwin G. Benedicto
Journal of the Philippine Medical Association 2019;97(2):52-60
BACKGROUND:
A reliable and socially validated definition of recovery in schizophrenia is essential to decrease
stigma associated with the illness. This study aimed to define recovery in schizophrenia in the Philippine context,
determine its specific elements, and describe methods of assessment in clinical practice.
METHODS:
We invited a group of purposively selected Filipino psychiatrists to participate in six simultaneous
roundtable discussions to gather their opinions and perspectives on recovery in schizophrenia. Transcripts of the
discussions were then subjected to framework analysis.
RESULTS AND CONCLUSION
Most Filipino psychiatrists were of the considered opinion that recovery in schizophrenia
is possible, and their vision of a recovered patient resembles a combination of psychological and medical models.
The mini-FROGS tool was deemed generally applicable in the Philippine setting except for self-esteem and sense of
independence primarily because it is difficult to evaluate. The SWN was received with mixed reactions among the
psychiatrists. Spirituality as an element of recovery and the family-oriented culture of the Filipinos were emphasized as important considerations in assessing patients. Other suggestions were given to tailor-fit these tools to
the Philippine context.
Schizophrenia
;
Philippines
6.Intersectoral collaborations for the prevention and control of Vector Borne diseases: A scoping review
Ma. Sophia Graciela L. Reyes ; Chelseah Denise H. Torres ; Amiel Nazer C. Bermudez ; Kim L. Cochon ; Evalyn A. Roxas ; Sophia Anne S.P. Liao ; Dorothy Jean N. Ortega ; Abegail Visia Marie C. Silang ; Deinzel R. Uezono ; Maria Sonia S. Salamat ; Carl Abelardo T. Antonio
Acta Medica Philippina 2019;53(4):315-326
Objectives:
This scoping review aimed to support a landscape analysis to identify lessons learned about intersectoral collaborations (ISCs) by describing their existing models in the context of dengue, malaria and yellow fever.
Methods:
A scoping review following the methodology of Joanna Briggs Institute was performed using the following inclusion criteria: studies involving humans; studies discussing intersectoral collaborations, malaria/dengue/yellow fever, and prevention or control at any level; and studies in countries endemic for the aforementioned diseases. Studies were screened using Covidence, while data were extracted using NVivo.
Results:
Of the 7,535 records retrieved, 69 were included in the qualitative analysis. Most ISCs were initiated by multilateral organizations and ministries of health, and none by communities. Strategies included advocacy, health education, research, public health measures, resource mobilization, service delivery and training; mostly employed on a community level. Monitoring and evaluation were mostly formative, ongoing, and participatory. Gaps included administrative and policy barriers, resource shortages, and inadequate research and training.
Conclusions
Multiple models of ISC exist in the literature. There is a need to develop a comprehensive framework for an effective and sustainable multisectoral approach for the prevention and control of VBDs ensuring adequate resources, active stakeholders, and strategies that span the entire socio-ecological spectrum.
Dengue
;
Disease Vectors
;
Intersectoral Collaboration
;
Malaria
;
Vector Borne Diseases
7.Efficacy of Clarithromycin versus Methylprednisolone in the treatment of non-eosinophilic Nasal Polyposis: A randomized controlled trial.
Jamilyn C GAMMAD ; Antonio H CHUA ; Charmaine S TEMPLONUEVO-FLORES
Philippine Journal of Otolaryngology Head and Neck Surgery 2018;33(2):6-13
OBJECTIVE: To compare the efficacy of Clarithromycin versus Methylprednisolone in the treatment of non-eosinophilic nasal polyposis.
DESIGN: Randomized Controlled Trial
SETTING: Tertiary Government Training Hospital
PARTICIPANTS: Forty two (42) patients with Chronic Rhinosinusitis with determination of eosinophil count. Both groups were further randomized into a treatment arm given Clarithromycin (CLA) 500mg/ day and another arm given Methylprednisolone (METH) 32 mg/ day tampering to 8 mg/ day for 15 days. All participants underwent pre- and post-treatment evaluation via anterior rhinoscopy, Sino-Nasal Outcome Test (SNOT-22) and Endoscopic Appearance (EA) Scoring. Date were encoded and subjected to statistical analysis using Mann-Whitney U test.
RESULTS: For the 9 participants in the non-eosinophilic group, 4 were given CLA and 5 were given METH. The CLA arm showed significant improvement in SNOT-22 scores by the 15th day (p= .007). The METH arm did not demonstrate significant improvement by the 7th (p= .44) or 15th day (p= .22). Comparison in the improvement in SNOT-22 scores between the two arms showed that on both 7th and 15th days, CLA outperformed METH (p= 0.26 and p= .004, respectively). For the EA scoring, both the CLA and METH groups significantly improved by the 7th (p= .27 and p= 0.017, respectively) and 15th day (p= .013 and p= .027, respectively). Comparison in the improvement of EA scores between the two arms showed significant difference on the 15th day (p= .01) with the CLA performing better than METH. Overall, the results suggest that the CLA arm performed significantly better than METH arm in the treatment of non-eosinophilic patients.
Of the 33 eosinophilic patients, 17 were given CLA and 16 were given METH. The CLA arm showed significant improvement in SNOT-22 scores by the 15th day (p< .001) while the METH arm both on 7th (p= .033) and 15th day (p< .001). Comparison of the improvement in SNOT-22 results between the two arms showed no significant difference (7th day p= .494; 15th day p= .587). For the EA scoring, both treatment groups showed significant improvement by the 7th and 15th day (p< .001). Comparison on the improvement in EA scores between the two arms showed significant difference (p< .001) on both 7th and 15th day, suggesting that METH was more effective than CLA. Overall, the results showed that both CLA and METH were effective in the treatment of eosinophilic nasal polyps. However, METH was significantly better than CLA in terms of superior EA scores.
CONCLUSION: In terms of improving symptoms and well-being, as well as decreasing nasal polyp size and reducing discharge and edema as reflected in superios SNOT 22 and EA scores, Clarithromycin was significantly more effective than Methylprednisolone in the treatment of non-eosinophilic nasal polyps. While both Clarithromycin and Methylprednisolone were shown to be effective in the treatment of eosinophilic nasal polyps, Methylprednisolone was significantly better in that Clarithromycin in terms of EA scores. A biopsy for tissue eosinophilic cell count prior to treatment is recommended to establish the predominant inflammatory cell in nasal polyps in order to provide appropriate targeted treatment, i.e. Clarithromycin for non-eosinophilic nasal polyps and Methylprednisolone for eosinophilic polyps.
Human ; Male ; Female ; Macrolides ; Clarithromycin ; Methylprednisolone ; Nasal Polyps ; Eosinophils
8.Efficacy of Clarithromycin versus Methylprednisolone in the Treatment of Non-Eosinophilic and Eosinophilic Nasal Polyposis: A Randomized Controlled Trial.
Jemilyn C. GAMMAD ; Antonio H. CHUA ; Charmaine S. TEMPLONUEVO-FLORES
Philippine Journal of Otolaryngology Head and Neck Surgery 2018;33(2):6-13
Objective: To compare the efficacy of Clarithromycin versus Methylprednisolone in the treatment of non-eosinophilic and eosinophilic nasal polyposis.
Methods:
Study Design: Randomized controlled trial
Setting: Tertiary Government Training Hospital
Subjects: Forty two patients with Chronic Rhinosinusitis with Nasal Polyps (CRSwNP) were grouped into non-eosinophilic and eosinophilic groups after biopsy determination of eosinophil count. Both groups were further randomized into a treatment arm given Clarithromycin (CLA) 500 mg/ day and another arm given Methylprednisolone (METH) 32 mg/ day tapering to 8 mg/ day for 15 days. All participants underwent pre- and post-treatment evaluation via anterior rhinoscopy, Sino-Nasal Outcome Test (SNOT-22) and Endoscopic Appearance (EA) Scoring. Data were encoded and subjected to statistical analysis using Mann-Whitney U test.
Results: For the 9 participants in the non-eosinophilic group, 4 were given CLA and 5 were given METH. The CLA arm showed significant improvement in SNOT-22 scores by the 15th day (p= .007). The METH arm did not demonstrate significant improvement by the 7th (p= .44) or 15th day (p= .22). Comparison of the improvement in SNOT-22 scores between the two arms showed that on both 7th and 15th days, CLA outperformed METH (p= .026 and p= .004, respectively). For the EA scoring, both the CLA and METH groups significantly improved by the 7th (p= .027 and p= 0.017, respectively), and 15th day (p= .013 and p= .027, respectively). Comparison of the improvement in EA scores between the two arms showed significant difference on the 15th day (p= .01), with the CLA performing better than METH. Overall, the results suggest that the CLA arm performed significantly better than the METH arm in the treatment of non-eosinophilic patients.
Of the 33 eosinophilic patients, 17 were given CLA and 16 were given METH. The CLA arm showed significant improvement in SNOT-22 scores by the 15th day (p < .001), while the METH arm on both 7th (p= .033) and 15th day (p< .001). Comparison of the improvement in SNOT-22 results between the two arms showed no significant differences (7th day p= .494; 15th day p= .587). For the EA scoring, both treatment groups showed significant improvement by the 7th and 15th day (p< .001). Comparison of the improvement in EA scores between the two arms showed significant differences (p< .001) on both 7th and 15th day, suggesting that METH was more effective than CLA. Overall, the results showed that both CLA and METH were effective in the treatment of eosinophilic nasal polyps. However, METH was significantly better than CLA in terms of superior EA scores.
Conclusion: In terms of improving symptoms and well-being, as well as decreasing nasal polyp size and reducing discharge and edema as reflected in superior SNOT-22 and EA scores, Clarithromycin was significantly more effective than Methylprednisolone in the treatment of non-eosinophilic nasal polyps. While both Clarithromycin and Methylprednisolone were shown to be effective in the treatment of eosinophilic nasal polyps, Methylprednisolone was significantly better than Clarithromycin in terms of superior EA scores. A biopsy for tissue eosinophil cell count prior to treatment is recommended to establish the predominant inflammatory cell in nasal polyps in order to provide appropriate targeted treatment, i.e. Clarithromycin for non-eosinophilic nasal polyps and Methylprednisolone for eosinophilic polyps.
Keywords: macrolides, clarithromycin, methylprednisolone, nasal polyps, eosinophils
Human ; Male ; Female ; Aged (a Person 65 Through 79 Years Of Age) ; Middle Aged (a Person 45-64 Years Of Age) ; Clarithromycin ; Macrolides ; Methylprednisolone ; Nasal Polyps ; Eosinophils
9.Unilateral pedunculated polyp of the palatine tonsil.
Daniel Jose C. Mendoza ; Antonio H. Chua ; Samantha S. Castañ ; eda
Philippine Journal of Otolaryngology Head and Neck Surgery 2015;30(1):51-53
OBJECTIVES: To report a benign tonsillar lesion presenting as a pedunculated polyp and discuss its diagnosis and management.
METHODS:
Design: Case Report
Setting: Tertiary Government Hospital
Patient: One
RESULTS: A 14-year-old lad presented with a seven-year history of an elongated right tonsillar mass without associated bleeding, pain, dysphagia or obstructive sleep apnea. Physical examination revealed a pedunculated mass about 2 x 1 x 0.5cm in size located in the superior pole. After unilateral tonsillectomy, histopathological examination revealed lymphangectatic lipomatous fibrotic polyp.
CONCLUSION: Lymphangiomatous polyp of the palatine tonsils is an unusual benign lesion of the head and neck. These are commonly present as unilateral, polypoidal mass that cannot be clinically differentiated from other benign tonsillar lesions. Tonsillectomy is the recommended surgical approach for both diagnostic and therapeutic purposes. Histopathological study must be done to confirm diagnosis.
Human ; Male ; Adolescent ; Palatine Tonsil ; Hamartoma ; Tonsillectomy ; Lymphangioma ; Deglutition Disorders ; Neck ; Head ; Sleep Apnea, Obstructive ; Polyps ; Pain ; Physical Examination
10.Inhibitory effect of Quassia amara Linn. crude bark extract on Entamoeba histolytica in vitro.
Panganiban Jayson C ; Patupat Annarose L ; Paulino Jose Antonio T ; Penserga Grace G ; Poncio Mar Aristeo G ; Porlas Romeo V ; Quezon Anna Sharmie C ; Quicho Hernane M ; Ramos Everly Faith P ; Remonte Edgar F ; Reyes Julianne Francesca F ; Rivera Adovich S ; Rivera Kay C ; Rivera Manuel Gregorio T ; Rogelio Paolo Nico A ; Sagayaga Hope M ; Santiago Maria Carmina L ; See John Patrick ; Siy Waldermar T ; Cagayan Faye S ; Maramba Cecile C ; Rivera Pilarita T
Acta Medica Philippina 2014;48(4):53-58
BACKGROUND: Entamoeba histolytica is an important etiologic agent of diarrhea. Globally, it is estimated to infect 40 to 50 million people and cause 40,000 to 100,000 deaths per year. Metronidazole is effective but can cause adverse reactions in certain individuals. In search of alternatives, traditional medicinal plants are being studied. Several plants in Family Simaroubaceae have shown anti-amoebic activity. Quassia amara, a member of this family has not been tested.
OBJECTIVE: To determine the effect of Q. amara crude extract on Entamoeba histolytica in vitro.
METHODS: Initial testing of 104 µg/ml ethanolic bark extract was performed. Counts were made after 72 hours. Three trials in triplicates were performed.
Nine (9) dilutions of extract were then tested (18.8 to 5,00 µg/ml). Test tubes were checked for viable amoeba after 24-hour and 72-hour incubation. Minimum inhibitory concentrations (MIC) were determined for the two incubation periods. At least two trials in triplicates for each dilution were performed. metronidazole served as positive control.
RESULTS: At 104 µg/ml incubated for 72 hours, no viable amoeba was obtained and counted. The MIC after 24 hours was 5,000 µg/ml, while the MIC at 72 hours was 37.5 µg/ml.
CONCLUSION: Q. amara crude extract has inhibitory effects on E. histolycain vitro.
Human ; Male ; Female ; Aged 80 And Over ; Aged ; Middle Aged ; Adult ; Young Adult ; Adolescent ; Child ; Child Preschool ; Infant ; Infant Newborn ; Quassia ; Metronidazole ; Entamoeba Histolytica ; Plants, Medicinal ; Amoeba ; Simaroubaceae ; Microbial Sensitivity Tests ; Diarrhea


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