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1.First Case Report of Bacteremia Due to Catabacter hongkongensis in a Korean Patient.

Yong Jun CHOI ; Eun Jeong WON ; Soo Hyun KIM ; Myung Geun SHIN ; Jong Hee SHIN ; Soon Pal SUH

Annals of Laboratory Medicine 2017;37(1):84-87

2.Analysis of the Vaginal Microbiome by Next-Generation Sequencing and Evaluation of its Performance as a Clinical Diagnostic Tool in Vaginitis.

Ki Ho HONG ; Sung Kuk HONG ; Sung Im CHO ; Eunkyung RA ; Kyung Hee HAN ; Soon Beom KANG ; Eui Chong KIM ; Sung Sup PARK ; Moon Woo SEONG

Annals of Laboratory Medicine 2016;36(5):441-449

BACKGROUND: Next-generation sequencing (NGS) can detect many more microorganisms of a microbiome than traditional methods. This study aimed to analyze the vaginal microbiomes of Korean women by using NGS that included bacteria and other microorganisms. The NGS results were compared with the results of other assays, and NGS was evaluated for its feasibility for predicting vaginitis. METHODS: In total, 89 vaginal swab specimens were collected. Microscopic examinations of Gram staining and microbiological cultures were conducted on 67 specimens. NGS was performed with GS junior system on all of the vaginal specimens for the 16S rRNA, internal transcribed spacer (ITS), and Tvk genes to detect bacteria, fungi, and Trichomonas vaginalis. In addition, DNA probe assays of the Candida spp., Gardnerella vaginalis, and Trichomonas vaginalis were performed. Various predictors of diversity that were obtained from the NGS data were analyzed to predict vaginitis. RESULTS: ITS sequences were obtained in most of the specimens (56.2%). The compositions of the intermediate and vaginitis Nugent score groups were similar to each other but differed from the composition of the normal score group. The fraction of the Lactobacillus spp. showed the highest area under the curve value (0.8559) in ROC curve analysis. The NGS and DNA probe assay results showed good agreement (range, 86.2-89.7%). CONCLUSIONS: Fungi as well as bacteria should be considered for the investigation of vaginal microbiome. The intermediate and vaginitis Nugent score groups were indistinguishable in NGS. NGS is a promising diagnostic tool of the vaginal microbiome and vaginitis, although some problems need to be resolved.
Area Under Curve ; Bacteria/*genetics/isolation & purification ; Bacterial Proteins/genetics ; Candida/*genetics/isolation & purification ; Female ; Fungal Proteins/genetics ; Gardnerella vaginalis/genetics/isolation & purification ; High-Throughput Nucleotide Sequencing ; Humans ; *Microbiota ; RNA, Ribosomal, 16S/chemistry/genetics/metabolism ; ROC Curve ; Sequence Analysis, DNA ; Trichomonas vaginalis/genetics/isolation & purification ; Vagina/*microbiology ; Vaginitis/*diagnosis/microbiology

3.Fusobacterium Isolates Recovered From Colonic Biopsies of Inflammatory Bowel Disease Patients in Korea.

Yangsoon LEE ; Chang Soo EUN ; A Reum LEE ; Chan Hyuk PARK ; Dong Soo HAN

Annals of Laboratory Medicine 2016;36(4):387-389

4.First Case Report of Human Infection With Ochrobactrum tritici Causing Bacteremia and Cholecystitis.

Duck Jin HONG ; Keon Han KIM ; Jung Ok KIM ; Jun Sung HONG ; Seok Hoon JEONG ; Kyungwon LEE

Annals of Laboratory Medicine 2016;36(3):278-280

5.Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea.

Bongyoung KIM ; Hyunjoo PAI ; Kwang hyun LEE ; Yangsoon LEE

Annals of Laboratory Medicine 2016;36(6):617-619

6.Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria.

Wonmok LEE ; Myungsook KIM ; Dongeun YONG ; Seok Hoon JEONG ; Kyungwon LEE ; Yunsop CHONG

Annals of Laboratory Medicine 2015;35(1):69-75

BACKGROUND: By conventional methods, the identification of anaerobic bacteria is more time consuming and requires more expertise than the identification of aerobic bacteria. Although the matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) systems are relatively less studied, they have been reported to be a promising method for the identification of anaerobes. We evaluated the performance of the VITEK MS in vitro diagnostic (IVD; 1.1 database; bioMerieux, France) in the identification of anaerobes. METHODS: We used 274 anaerobic bacteria isolated from various clinical specimens. The results for the identification of the bacteria by VITEK MS were compared to those obtained by phenotypic methods and 16S rRNA gene sequencing. RESULTS: Among the 249 isolates included in the IVD database, the VITEK MS correctly identified 209 (83.9%) isolates to the species level and an additional 18 (7.2%) at the genus level. In particular, the VITEK MS correctly identified clinically relevant and frequently isolated anaerobic bacteria to the species level. The remaining 22 isolates (8.8%) were either not identified or misidentified. The VITEK MS could not identify the 25 isolates absent from the IVD database to the species level. CONCLUSIONS: The VITEK MS showed reliable identifications for clinically relevant anaerobic bacteria.
Bacteria, Anaerobic/*genetics/isolation & purification ; Bacterial Typing Techniques/*instrumentation/*methods ; Body Fluids/microbiology ; Databases, Genetic ; Humans ; RNA, Ribosomal, 16S/*analysis/metabolism ; Sequence Analysis, DNA ; *Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

1.First Case Report of Bacteremia Due to Catabacter hongkongensis in a Korean Patient.

2.Analysis of the Vaginal Microbiome by Next-Generation Sequencing and Evaluation of its Performance as a Clinical Diagnostic Tool in Vaginitis.

3.Fusobacterium Isolates Recovered From Colonic Biopsies of Inflammatory Bowel Disease Patients in Korea.

4.First Case Report of Human Infection With Ochrobactrum tritici Causing Bacteremia and Cholecystitis.

5.Identification of Pasteurella canis in a Soft Tissue Infection Caused by a Dog Bite: The First Report in Korea.

6.Evaluation of VITEK Mass Spectrometry (MS), a Matrix-Assisted Laser Desorption Ionization Time-of-Flight MS System for Identification of Anaerobic Bacteria.

7.Flavobacterium ceti From Blood Samples of a Korean Patient With Alcoholic Liver Cirrhosis.

8.First Case of Pulmonary Mycobacterium parascrofulaceum Infection in a Patient With Bronchiectasis in Korea.

9.Campylobacter hyointestinalis Isolated From a Human Stool Specimen.

10.The First Case of Concurrent Infective Endocarditis and Spondylitis Caused by Streptococcus tigurinus.

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